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Literature summary for 3.4.11.13 extracted from
- Sequential hydrolysis of proline-containing peptides with immobilized aminopeptidases (1983), Biochim. Biophys. Acta, 743, 437-446.
Application
| Application | Comment | Organism |
|---|---|---|
| analysis | immobilized enzyme can be used for peptide sequence determination, together with other peptidases, reactive in solutions containing up to 10% DMSO | Hathewaya histolytica |
General Stability
| General Stability | Organism |
|---|---|
| immobilization increases stability, e.g. to SDS | Hathewaya histolytica |
| irreversible inactivation at 0.5% M urea | Hathewaya histolytica |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.5 | 3 | L-leucine 4-nitroanilide | - |
Hathewaya histolytica |  |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Cd2+ | - |
Hathewaya histolytica | |
| Ni2+ | - |
Hathewaya histolytica | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Peptides + H2O | Hathewaya histolytica | - |
? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Hathewaya histolytica | - |
enzyme free or immobilized to amino glass beads | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
activity of immobilized enzyme 18% of that of soluble enzyme | Hathewaya histolytica |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, sodium barbital, pH 8.2, 50 mM Na-citrate, 10 mM CoSO4, 10% DMSO, several weeks, no loss of activity | Hathewaya histolytica |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | no endopeptidase activity | Hathewaya histolytica | ? | - |
? | |
| additional information | also hydrolysed: reduced or carboxymethylated bovine insulin B, degradation of the generally hydrolysis-resistant bradykinin nonapeptide in concert with aminopeptidase-p | Hathewaya histolytica | ? | - |
? | |
| Peptides + H2O | - |
Hathewaya histolytica | ? | - |
? | |
| Pro-Gly-Pro + H2O | standadard substrate | Hathewaya histolytica | Pro + Gly-Pro | - |
ir | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
assay at | Hathewaya histolytica |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
inactivated above, immobilized enzyme | Hathewaya histolytica |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.2 | - |
assay at | Hathewaya histolytica |
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