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Literature summary for 3.4.11.10 extracted from

  • Maekinen, K.K.; Maekinen, P.L.; Wilkes, S.H.; Bayliss, M.E.; Prescott, J.M.
    Photochemical inactivation of Aeromonas aminopeptidase by 2,3-butanedione (1982), J. Biol. Chem., 257, 1765-1772.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
1,2-Cyclohexanedione Ki: 0.022-0.023 mM Vibrio proteolyticus
2,3-Butanedione photochemical inactivation: effect only in the light, proportional to light intensity, modification of 5 Trp-, 3-4 Tyr-, 2 His- and 2Arg-residues, no photoinactivation in the absence of oxygen or in the presence of azide, protection also by Trp, Met, D-Met, L-2-thiol-His, 2-mercaptoethanol, Gly-Met, Ki: 5.1-19.8 mM Vibrio proteolyticus
2,3-Pentanedione photochemical inactivation: effect only in UV-light Vibrio proteolyticus
3-methyl-1,2-cyclopentanedione Ki: 0.48-1.19 mM Vibrio proteolyticus
Glyoxal Ki: 3.7-6.5 mM Vibrio proteolyticus
methylglyoxal photochemical inactivation: effect only in UV-light, Ki: 1.8-2.0 mM Vibrio proteolyticus
Phenylglyoxal Ki: 0.002-0.0023 mM Vibrio proteolyticus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.021
-
L-leucine 4-nitroanilide native enzyme Vibrio proteolyticus
0.126
-
L-leucine 4-nitroanilide photoinactivated enzyme Vibrio proteolyticus

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ no loss of zinc upon photoinactivation Vibrio proteolyticus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Peptides + H2O Vibrio proteolyticus
-
?
-
?

Organism

Organism UniProt Comment Textmining
Vibrio proteolyticus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Peptides + H2O
-
Vibrio proteolyticus ?
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1
-
L-leucine 4-nitroanilide photoinactivated enzyme Vibrio proteolyticus
67.3
-
L-leucine 4-nitroanilide native enzyme Vibrio proteolyticus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Vibrio proteolyticus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.002 0.0023 Phenylglyoxal
-
Vibrio proteolyticus
0.022 0.023 1,2-Cyclohexanedione
-
Vibrio proteolyticus
0.48 1.19 3-methyl-1,2-cyclopentanedione
-
Vibrio proteolyticus
1.8 2 methylglyoxal photochemical inactivation: effect only in UV-light Vibrio proteolyticus
3.7 6.5 Glyoxal
-
Vibrio proteolyticus
5.1 19.8 2,3-Butanedione photochemical inactivation: effect only in the light, proportional to light intensity, modification of 5 Trp-, 3-4 Tyr-, 2 His- and 2Arg-residues, no photoinactivation in the absence of oxygen or in the presence of azide, protection also by Trp, Met, D-Met, L-2-thiol-His, 2-mercaptoethanol, Gly-Met Vibrio proteolyticus