General Stability | Organism |
---|---|
only partially inactivated in 8 M urea | Vibrio proteolyticus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | - |
Vibrio proteolyticus | |
EDTA | - |
Vibrio proteolyticus | |
Leu-bromomethyl ketone | succinamic acid derivative, Ki: 0.0069 mM | Vibrio proteolyticus | |
Leu-methyl ketone | derivatives, Ki: 0.14 - 0.17 mM | Vibrio proteolyticus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | amides and dipeptides | Vibrio proteolyticus | |
0.019 | - |
L-leucine 4-nitroanilide | - |
Vibrio proteolyticus | |
0.18 | - |
Leu-Leu | - |
Vibrio proteolyticus | |
0.35 | - |
Leu-Met | - |
Vibrio proteolyticus | |
0.38 | - |
Leu-Ile | - |
Vibrio proteolyticus | |
0.39 | - |
Leu-Arg | - |
Vibrio proteolyticus | |
0.43 | - |
Leu-beta-naphthylamide | - |
Vibrio proteolyticus | |
0.64 | - |
Leu-Val | - |
Vibrio proteolyticus | |
0.86 | - |
Leu-Phe | - |
Vibrio proteolyticus | |
0.96 | - |
Leu-Trp | - |
Vibrio proteolyticus | |
1 | - |
Leu-Ala | - |
Vibrio proteolyticus | |
1.5 | - |
Leu-Tyr | - |
Vibrio proteolyticus | |
3.9 | - |
Phe-methyl ester | - |
Vibrio proteolyticus | |
5.1 | - |
Leu-amide | - |
Vibrio proteolyticus | |
6.3 | - |
norleucinamide | - |
Vibrio proteolyticus | |
8.5 | - |
Leu-methyl ester | - |
Vibrio proteolyticus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Vibrio proteolyticus | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | - |
Vibrio proteolyticus | |
Mn2+ | - |
Vibrio proteolyticus | |
Zn2+ | - |
Vibrio proteolyticus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
28480 | - |
sedimentation equilibrium | Vibrio proteolyticus |
29500 | - |
sedimentation velocity | Vibrio proteolyticus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
Peptides + H2O | Vibrio proteolyticus | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Vibrio proteolyticus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Vibrio proteolyticus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
35.5 | - |
- |
Vibrio proteolyticus |
Storage Stability | Organism |
---|---|
-20°C, 10 M tricine buffer, pH 8.0-8.5, several years, little loss of activity | Vibrio proteolyticus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Leu-4-nitroanilide + H2O | - |
Vibrio proteolyticus | Leu + 4-nitroaniline | - |
ir | |
Leu-Ala + H2O | - |
Vibrio proteolyticus | Leu + Ala | - |
ir | |
Leu-amide + H2O | - |
Vibrio proteolyticus | Leu + NH3 | - |
ir | |
Leu-Arg + H2O | - |
Vibrio proteolyticus | Leu + Arg | - |
ir | |
Leu-beta-naphthylamide + H2O | - |
Vibrio proteolyticus | Leu + beta-naphthylamine | - |
ir | |
Leu-Ile + H2O | - |
Vibrio proteolyticus | Leu + Ile | - |
ir | |
Leu-Leu + H2O | - |
Vibrio proteolyticus | Leu + Leu | - |
ir | |
Leu-Met + H2O | - |
Vibrio proteolyticus | Leu + Met | - |
ir | |
Leu-methyl ester + H2O | - |
Vibrio proteolyticus | Leu + methanol | - |
ir | |
Leu-Phe + H2O | - |
Vibrio proteolyticus | Leu + Phe | - |
ir | |
Leu-Trp + H2O | - |
Vibrio proteolyticus | Leu + Trp | - |
ir | |
Leu-Tyr + H2O | - |
Vibrio proteolyticus | Leu + Tyr | - |
ir | |
Leu-Val + H2O | - |
Vibrio proteolyticus | Leu + Val | - |
ir | |
additional information | also cleaved: gastrin peptide (carboxyterminal), various oligopeptides, Met-Lys-bradykinin, adrenocorticotropic hormone, glucagon, aminoethylated B-chain insulin, sperm whale myoglobin, ribunuclease, beta-lactoglobulin, egg white lysozyme, aminoethylated lysozyme | Vibrio proteolyticus | ? | - |
? | |
norleucinamide + H2O | - |
Vibrio proteolyticus | norleucine + NH3 | - |
ir | |
Peptides + H2O | - |
Vibrio proteolyticus | ? | - |
? | |
Phe-Asp-Ser-Ala-Val + H2O | - |
Vibrio proteolyticus | Phe + Asp-Ser-Ala-Val | - |
ir | |
Phe-methyl ester + H2O | - |
Vibrio proteolyticus | Phe + methanol | - |
ir | |
Pro-Phe-Gly-Lys + H2O | - |
Vibrio proteolyticus | Pro + Phe-Gly-Lys | - |
ir | |
Pro-Phe-Pro + H2O | - |
Vibrio proteolyticus | Pro + Phe-Pro | - |
ir |
Subunits | Comment | Organism |
---|---|---|
monomer | SDS-PAGE | Vibrio proteolyticus |
More | amino acid composition | Vibrio proteolyticus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Vibrio proteolyticus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
below, but tolerates exposure up to a few hours | Vibrio proteolyticus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
8.3 | - |
Leu-Leu | - |
Vibrio proteolyticus | |
15 | - |
Leu-Ala | - |
Vibrio proteolyticus | |
15 | - |
Leu-Val | - |
Vibrio proteolyticus | |
16 | - |
Leu-Ile | - |
Vibrio proteolyticus | |
29.6 | - |
Leu-beta-naphthylamide | - |
Vibrio proteolyticus | |
39 | - |
Leu-Arg | - |
Vibrio proteolyticus | |
42 | - |
Phe-methyl ester | - |
Vibrio proteolyticus | |
53 | - |
Leu-Met | - |
Vibrio proteolyticus | |
58 | - |
Leu-Trp | - |
Vibrio proteolyticus | |
60 | - |
norleucinamide | - |
Vibrio proteolyticus | |
67 | - |
L-leucine 4-nitroanilide | - |
Vibrio proteolyticus | |
72 | - |
Leu-Phe | - |
Vibrio proteolyticus | |
85 | - |
Leu-Tyr | - |
Vibrio proteolyticus | |
145 | - |
Leu-methyl ester | - |
Vibrio proteolyticus | |
220 | - |
Leu-amide | - |
Vibrio proteolyticus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Vibrio proteolyticus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
8 | 8.5 | - |
Vibrio proteolyticus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0002 | - |
Leu-bromomethyl ketone | - |
Vibrio proteolyticus |