Literature summary for 3.4.11.10 extracted from

Perez-Sanchez, G.; Leal-Guadarrama, L.; Trelles, I.; Perez, N.; Medina-Rivero, E.
High-level production of a recombinant Vibrio proteolyticus leucine aminopeptidase and its use for N-terminal methionine excision from interferon alpha-2b (2011), Process Biochem., 46, 1825-1830.

No PubMed abstract available

Search for more literature for 3.4.11.10

Cloned(Commentary)

Cloned (Comment)	Organism
His-tagged protein expressed in Escherichia coli MM294	Vibrio proteolyticus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.02726	-	recombinant human methionine-interferon alpha-2b	pH 6.0, 37°C	Vibrio proteolyticus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
32000	-	x * 32000, SDS-PAGE	Vibrio proteolyticus

Organism

Organism	UniProt	Comment	Textmining
Vibrio proteolyticus	Q01693	-	-

Purification (Commentary)

Purification (Comment)	Organism
from inclusion bodies, immobilized metal ion affinity chromatography (Ni2+), purity of 95%	Vibrio proteolyticus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
16	-	substrate L-leucine-4-nitroanilidepH 8.0, 37°C	Vibrio proteolyticus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-leucine-4-nitroanilide + H2O	-	Vibrio proteolyticus	L-leucine + 4-nitroaniline	-	?
recombinant human methionine-interferon alpha-2b + H2O	-	Vibrio proteolyticus	L-methionine + recombinant human interferon alpha-2b	-	?

Subunits

Subunits	Comment	Organism
?	x * 32000, SDS-PAGE	Vibrio proteolyticus

Synonyms

Synonyms	Comment	Organism
leucine aminopeptidase	-	Vibrio proteolyticus

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	-	Vibrio proteolyticus

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.000853	-	recombinant human methionine-interferon alpha-2b	pH 6.0, 37°C	Vibrio proteolyticus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6	-	-	Vibrio proteolyticus

pH Range

pH Minimum	pH Maximum	Comment	Organism
4	9	-	Vibrio proteolyticus

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Release 2023.1 (January 2023)