Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Treponema denticola |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 22% residual activity in the presence of 2.0 mM Ca2+ | Treponema denticola | |
EDTA | 2% residual activity in the presence of 0.2 mM EDTA | Treponema denticola | |
Zn2+ | 23% residual activity in the presence of 2.0 mM Zn2+ | Treponema denticola |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.39 | - |
Cys-Gly | recombinant enzyme, at pH 8.0 | Treponema denticola | |
0.44 | - |
Cys-Gly | recombinant enzyme, at pH 7.3 | Treponema denticola | |
0.48 | - |
Cys-Gly | native enzyme, at pH 7.3 | Treponema denticola | |
1.72 | - |
L-Leu-p-nitroanilide | recombinant enzyme, at pH 7.3 | Treponema denticola | |
1.79 | - |
L-Leu-p-nitroanilide | recombinant enzyme, at pH 8.0 | Treponema denticola |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 580% relative activity in the presence of 2.0 mM Co2+ | Treponema denticola | |
Mg2+ | 258% relative activity in the presence of 2.0 mM Mg2+ | Treponema denticola | |
Mn2+ | 510% relative activity in the presence of 2.0 mM Mn2+ | Treponema denticola | |
additional information | not stimulated by Zn2+ or Ca2+ | Treponema denticola |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
52000 | - |
SDS-PAGE | Treponema denticola |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Treponema denticola | - |
strain ATCC 35404 | - |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, ultrafiltration, and Sepharose Q column chromatography | Treponema denticola |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Cys-Gly + H2O | preferred substrate | Treponema denticola | L-Cys + Gly | - |
? | |
glutathione + H2O | the enzyme is involved in turnover of glutathione | Treponema denticola | ? | - |
? | |
L-Leu-p-nitroanilide + H2O | - |
Treponema denticola | L-Leu + p-nitroaniline | - |
? | |
additional information | no activity towards Asp-Gly, Glu-Cys-Gly, and L-Cys | Treponema denticola | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CGase | the protein is a member of the family of metalloproteases called M17 leucine aminopeptidases | Treponema denticola |
cysteinylglycinase | - |
Treponema denticola |
Leucyl aminopeptidase | - |
Treponema denticola |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
30 | - |
L-Leu-p-nitroanilide | recombinant enzyme, at pH 7.3 | Treponema denticola | |
41.7 | - |
L-Leu-p-nitroanilide | recombinant enzyme, at pH 8.0 | Treponema denticola | |
43.3 | - |
Cys-Gly | recombinant enzyme, at pH 8.0 | Treponema denticola | |
46.7 | - |
Cys-Gly | native enzyme, at pH 7.3 | Treponema denticola | |
60 | - |
Cys-Gly | recombinant enzyme, at pH 7.3 | Treponema denticola |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.3 | - |
the activity of CGase for Cys-Gly is optimum at pH 7.3 | Treponema denticola |
8 | - |
the activity of CGase for L-Leu-p-nitroanilide is optimum at pH 8.0 | Treponema denticola |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7.3 | 8 | the amidolytic activity of the enzyme toward Cys-Gly is maximal at pH 7.3/7.4 and declines fairly rapidly at higher or lower pH conditions, the activity of the enzyme with L-Leu-p-nitroanilide is not as sensitive to increases or decreases in pH | Treponema denticola |