Application | Comment | Organism |
---|---|---|
pharmacology | the enzyme is a target for development of anti-Helicobacter pylori agents | Helicobacter pylori |
Cloned (Comment) | Organism |
---|---|
gene LAP, DNA and amino acid sequence determination and analysis, functional expression of His-tagged enzyme in Escherichia coli | Helicobacter pylori |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
bestatin | inhibits the enzyme activity and cell growth | Helicobacter pylori | |
Ca2+ | 78% inhibition at 0.1 mM, 94% inhibition at 1 mM, 96% inhibition at 10 mM | Helicobacter pylori | |
EDTA | 97% inhibition at 0.1 mM | Helicobacter pylori | |
additional information | no inhibition by PMSF | Helicobacter pylori | |
Zn2+ | 65% inhibition at 0.1 mM, 78% inhibition at 1 mM, 91% inhibition at 10 mM | Helicobacter pylori |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | highly activating, about 5fold at 1-10 mM | Helicobacter pylori | |
Mg2+ | activates 2.9fold at 1 mM, 3.7fold at 10 mM | Helicobacter pylori | |
Mn2+ | highly activating, 5.6fold at 1-10 mM | Helicobacter pylori | |
Ni2+ | activates 3.5fold at 1 mM, 3.8fold at 10 mM | Helicobacter pylori |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55000 | - |
6 * 55000, recombinant LAP, SDS-PAGE | Helicobacter pylori |
340000 | - |
recombinant enzyme, gel filtration | Helicobacter pylori |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Helicobacter pylori | - |
gene LAP | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-Leu-4-nitroanilide + H2O | - |
Helicobacter pylori | L-Leu + 4-nitroaniline | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | 6 * 55000, recombinant LAP, SDS-PAGE | Helicobacter pylori |
More | the monomeric enzyme is probably not active | Helicobacter pylori |
Synonyms | Comment | Organism |
---|---|---|
LAP | - |
Helicobacter pylori |
Leucyl aminopeptidase | - |
Helicobacter pylori |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
recombinant LAP | Helicobacter pylori |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
15 | 100 | temperature profile, about 60% of maximal activity at 15°C and 100°C, recombinant enzyme | Helicobacter pylori |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
recombinant LAP | Helicobacter pylori |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 10 | pH profile, about 40% of maximal activity at pH 5.5 and pH 10.0, rapid decline of activity at pH 5.0, recombinant enzyme | Helicobacter pylori |