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Literature summary for 3.4.11.10 extracted from
- Purification and properties of two intracellular aminopeptidases produced by Brevibacterium linens SR3 (2000), Int. Dairy J., 10, 241-248.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,10-phenanthroline | strong inhibition | Brevibacterium linens |  |
| Ca2+ | - |
Brevibacterium linens | |
| Cu2+ | - |
Brevibacterium linens | |
| cysteine | strong inhibition | Brevibacterium linens | |
| EDTA | strong inhibition | Brevibacterium linens | |
| Fe2+ | - |
Brevibacterium linens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.25 | - |
L-Leu-4-nitroanilide | pH 8.0, 37°C, APII | Brevibacterium linens | |
| 1 | - |
L-Leu-4-nitroanilide | pH 8.5, 45°C, API | Brevibacterium linens | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| intracellular | - |
Brevibacterium linens | 5622 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | activates | Brevibacterium linens | |
| additional information | API and APII are metallopeptidases | Brevibacterium linens | |
| Ni2+ | activates | Brevibacterium linens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 14000 | - |
x * 14000, API, SDS-PAGE, x * 18000, APII, SDS-PAGE | Brevibacterium linens |
| 18000 | - |
x * 14000, API, SDS-PAGE, x * 18000, APII, SDS-PAGE | Brevibacterium linens |
| 80000 | - |
API, gel filtration | Brevibacterium linens |
| 220000 | - |
APII, gel filtration | Brevibacterium linens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Brevibacterium linens | - |
- |
- |
| Brevibacterium linens SR3 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native API and APII to homogeneity by hydrophobic interaction and ion exchange chromatography, and gel filtration | Brevibacterium linens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-Leu-4-nitroanilide + H2O | - |
Brevibacterium linens | L-Leu + 4-nitroaniline | - |
? | |
| L-Leu-4-nitroanilide + H2O | - |
Brevibacterium linens SR3 | L-Leu + 4-nitroaniline | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| oligomer | x * 14000, API, SDS-PAGE, x * 18000, APII, SDS-PAGE | Brevibacterium linens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| API | - |
Brevibacterium linens |
| APII | - |
Brevibacterium linens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
APII | Brevibacterium linens |
| 45 | - |
API | Brevibacterium linens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
APII | Brevibacterium linens |
| 8.5 | - |
API | Brevibacterium linens |
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