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Literature summary for 3.4.11.10 extracted from
- The thermophilic, homohexameric aminopeptidase of Borrelia burgdorferi is a member of the M29 family of metallopeptidases (2005), Infect. Immun., 73, 2253-2261.
Application
| Application | Comment | Organism |
|---|---|---|
| pharmacology | the enzyme ia a target for development of drugs in therapy of Lyme disease caused by Borrelia burgdorferi | Borreliella burgdorferi |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,10-phenanthroline | complete inhibition at 0.25 mM, reversible by Zn2+, not by other divalent cations, overview | Borreliella burgdorferi |  |
| bestatin | complete inhibition at 0.1 mM, not reversible by divalent cations | Borreliella burgdorferi | |
| EDTA | 50% inhibition at 10 mM, reversible by divalent cations, best by Zn2+ | Borreliella burgdorferi | |
| additional information | no or poor inhibition by PMSF, E64, TLCK, pepstatin A, and leupeptin | Borreliella burgdorferi |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Zn2+ | dependent on | Borreliella burgdorferi | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 50000 | - |
6 * 50000, SDS-PAGE | Borreliella burgdorferi |
| 300000 | - |
gel filtration | Borreliella burgdorferi |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Borreliella burgdorferi | the enzyme is probably involved in amino acid supply in the organism deficient in amino acid synthesis pathways, and/or in peptide and/or protein processing | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Borreliella burgdorferi | O51096 | gene BB0069 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme 32fold to homogeneity by anion exchange chromatography and gel filtration | Borreliella burgdorferi |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.001 | - |
purified enzyme | Borreliella burgdorferi |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-Leu-7-amido-4-methylcoumarin + H2O | - |
Borreliella burgdorferi | L-Leu + 7-amino-4-methylcoumarin | - |
? | |
| additional information | the enzyme is probably involved in amino acid supply in the organism deficient in amino acid synthesis pathways, and/or in peptide and/or protein processing | Borreliella burgdorferi | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| hexamer | 6 * 50000, SDS-PAGE | Borreliella burgdorferi |
| More | oligomerization is required for activity, the monomer is inactive, the enzyme contains no disulfide bonds, peptide mass fingerprinting, overview | Borreliella burgdorferi |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| aminopeptidase II | - |
Borreliella burgdorferi |
| More | the enzyme is a member of the M29 family of metallopeptidases | Borreliella burgdorferi |
| TAP | - |
Borreliella burgdorferi |
| thermophilic aminopeptidase | - |
Borreliella burgdorferi |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Borreliella burgdorferi |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
thermal inactivation through transition of the hexameric to the monomeric form | Borreliella burgdorferi |
| 25 | 50 | purified enzyme, completely stable for 240 min | Borreliella burgdorferi |
| 60 | - |
purified enzyme, no loss activity within 10 min, 240 min: loss of 12.5% activity | Borreliella burgdorferi |
| 70 | - |
purified enzyme, loss of 75% activity within 10 min, 240 min: loss of 91% activity | Borreliella burgdorferi |
| 100 | - |
purified enzyme, 10 min, complete inactivation | Borreliella burgdorferi |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Borreliella burgdorferi |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 9 | 20% of maximal activity at pH 6.0, 50% at pH 9.0 | Borreliella burgdorferi |
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Release 2023.1 (January 2023)
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