Crystallization (Comment) | Organism |
---|---|
10 mg/ml purified enzyme, complexed with inhibitor L-leucinephosphonic acid from a 4fold molar excess, with precipitation solution containing Tris, pH 8.0, 10 mM KSCN, 0.4 M NaCl, 4 days, X-ray diffraction structure determination and analysis at 2.1 A resolution | Vibrio proteolyticus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
L-leucine 4-nitroanilide | - |
Vibrio proteolyticus | |
L-leucinephosphonic acid | competitive, interacts with both metal ions in the dinuclear active site, inhibition mechanism | Vibrio proteolyticus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics with metal-bound enzyme variants | Vibrio proteolyticus | |
0.01 | - |
L-leucine 4-nitroanilide | pH 8.0, 25°C | Vibrio proteolyticus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 2 Co2+ bound at the dinuclear active site, can be substituted by Zn2+, binding mode | Vibrio proteolyticus | |
Zn2+ | 2 Zn2+ bound at the dinuclear active site, can be substituted by Co2+, binding mode | Vibrio proteolyticus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Vibrio proteolyticus | Q01693 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Vibrio proteolyticus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
Release of an N-terminal amino acid, preferentially leucine, but not glutamic or aspartic acids. | active site structure and mechanism | Vibrio proteolyticus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
120 | - |
purified Zn2+-bound enzyme | Vibrio proteolyticus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-leucine 4-nitroanilide + H2O | - |
Vibrio proteolyticus | L-leucine + 4-nitroaniline | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AAP | - |
Vibrio proteolyticus |
Aeromonas proteolytica aminopeptidase | - |
Vibrio proteolyticus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Vibrio proteolyticus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4320 | - |
L-leucine 4-nitroanilide | pH 8.0, 25°C | Vibrio proteolyticus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Vibrio proteolyticus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics with metal-bound enzyme variants | Vibrio proteolyticus | |
0.0055 | - |
L-leucine 4-nitroanilide | enzyme with 2 Co2+ or Zn2+ bound, pH 8.0, 25°C | Vibrio proteolyticus | |
0.0066 | - |
L-leucinephosphonic acid | metal free enzyme, pH 8.0, 25°C | Vibrio proteolyticus |