Literature summary for 3.4.11.1 extracted from

Wu, M.; Yan, M.; Xu, J.; Liang, Y.; Gu, X.; Xie, Y.; Jing, B.; Lai, W.; Peng, X.; Yang, G.
Expression, tissue localization and serodiagnostic potential of Echinococcus granulosus leucine aminopeptidase (2018), Int. J. Mol. Sci., 19, 1063-1075 .

Search for more literature for 3.4.11.1

Cloned(Commentary)

Cloned (Comment)	Organism
gene LAP, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)	Echinococcus granulosus

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
additional information	no transmembrane regions or signal peptide sequences are predicted in the deduced amino acid sequence	Echinococcus granulosus	-	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Co2+	activates	Echinococcus granulosus
Mn2+	activates	Echinococcus granulosus
Ni2+	activates	Echinococcus granulosus

Organism

Organism	UniProt	Comment	Textmining
Echinococcus granulosus	A0A336UEJ6	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography	Echinococcus granulosus

Source Tissue

Source Tissue	Comment	Organism	Textmining
additional information	localization of the enzyme protein in the parasite in sheep using fluorescence immunohistochemistry, development of an ELISA method, efficient and effective for early stage diagnosis of infection and the detection of infected animals, overview	Echinococcus granulosus	-

Subunits

Subunits	Comment	Organism
homohexamer	6 * 65800, about, sequence calculation, 6 * 66000, native enzyme, SDS-PAGE, 6 * 72000, recombinant His-tagged enzyme, SDS-PAGE	Echinococcus granulosus

Synonyms

Synonyms	Comment	Organism
Eg-LAP	-	Echinococcus granulosus
LAP	-	Echinococcus granulosus
leucine aminopeptidase	-	Echinococcus granulosus

pI Value

Organism	Comment	pI Value Maximum	pI Value
Echinococcus granulosus	sequence calculation	-	6.6

General Information

General Information	Comment	Organism
evolution	the enzyme belongs to the M17 peptidase family	Echinococcus granulosus
physiological function	leucine aminopeptidases (LAPs) of the M17 peptidase family have important functions in regulating the balance of catabolism and anabolism, cell maintenance, growth and defense	Echinococcus granulosus

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Release 2023.1 (January 2023)