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Literature summary for 3.4.11.1 extracted from

  • Kang, J.; Ju, H.; Ju, J.; Sohn, W.; Kim, T.; Bahk, Y.; Hong, S.; Na, B.
    Comparative biochemical and functional properties of two leucine aminopeptidases of Clonorchis sinensis (2012), Mol. Biochem. Parasitol., 182, 17-26.
    View publication on PubMed
Search for more literature for 3.4.11.1

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli as a His-tagged fusion protein Clonorchis sinensis

Inhibitors

Inhibitors Comment Organism Structure
amastatin
-
 Clonorchis sinensis
bestatin
-
 Clonorchis sinensis
EDTA
-
 Clonorchis sinensis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.035
-
 L-Leu-4-nitroanilide pH 8, 20°C Clonorchis sinensis
0.036
-
 L-Leu-4-nitroanilide pH 8, 20°C Clonorchis sinensis
0.053
-
 L-Met-4-nitroanilide pH 8, 20°C Clonorchis sinensis
0.0597
-
 L-Met-4-nitroanilide pH 8, 20°C Clonorchis sinensis
0.0842
-
 L-Phe-4-nitroanilide pH 8, 20°C Clonorchis sinensis
0.091
-
 L-Phe-4-nitroanilide pH 8, 20°C Clonorchis sinensis
0.094
-
 L-Lys-4-nitroanilide pH 8, 20°C Clonorchis sinensis
0.103
-
 L-Lys-4-nitroanilide pH 8, 20°C Clonorchis sinensis
0.185
-
 L-Ala-4-nitroanilide pH 8, 20°C Clonorchis sinensis
0.198
-
 L-Thr-4-nitroanilide pH 8, 20°C Clonorchis sinensis
0.202
-
 L-Ala-4-nitroanilide pH 8, 20°C Clonorchis sinensis
0.204
-
 L-Thr-4-nitroanilide pH 8, 20°C Clonorchis sinensis
0.216
-
 L-Pro-4-nitroanilide pH 8, 20°C Clonorchis sinensis
0.239
-
 L-Pro-4-nitroanilide pH 8, 20°C Clonorchis sinensis
0.261
-
 L-Ile-4-nitroanilide pH 8, 20°C Clonorchis sinensis
0.372
-
 L-Val-4-nitroanilide pH 8, 20°C Clonorchis sinensis

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ enhances activity Clonorchis sinensis
Mn2+ enhances activity Clonorchis sinensis
Zn2+ enhances activity Clonorchis sinensis

Organism

Organism UniProt Comment Textmining
Clonorchis sinensis C6FWG9
-
-
Clonorchis sinensis C6FWH0
-
-

Purification (Commentary)

Purification (Comment) Organism
using Ni-NTA chromatography Clonorchis sinensis

Source Tissue

Source Tissue Comment Organism Textmining
epithelial cell co-localizes with CsLAP1 to the intestinal epithelial cells of the parasite Clonorchis sinensis
-
epithelial cell co-localizes with CsLAP2 to the intestinal epithelial cells of the parasite Clonorchis sinensis
-
gastrodermis co-localizes with CsLAP1 to the gastrodermis of the parasite Clonorchis sinensis
-
gastrodermis co-localizes with CsLAP2 to the gastrodermis of the parasite Clonorchis sinensis
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-Ala-4-nitroanilide + H2O
-
 Clonorchis sinensis L-Ala + 4-nitroaniline
-
 ?
L-Ile-4-nitroanilide + H2O
-
 Clonorchis sinensis L-Ile + 4-nitroaniline
-
 ?
L-Leu-4-nitroanilide + H2O
-
 Clonorchis sinensis L-Leu + 4-nitroaniline
-
 ?
L-Lys-4-nitroanilide + H2O
-
 Clonorchis sinensis L-Lys + 4-nitroaniline
-
 ?
L-Met-4-nitroanilide + H2O
-
 Clonorchis sinensis L-Met + 4-nitroaniline
-
 ?
L-Phe-4-nitroanilide + H2O
-
 Clonorchis sinensis L-Phe + 4-nitroaniline
-
 ?
L-Pro-4-nitroanilide + H2O
-
 Clonorchis sinensis L-Pro + 4-nitroaniline
-
 ?
L-Thr-4-nitroanilide + H2O
-
 Clonorchis sinensis L-Thr + 4-nitroaniline
-
 ?
L-Val-4-nitroanilide + H2O
-
 Clonorchis sinensis L-Val + 4-nitroaniline
-
 ?

Synonyms

Synonyms Comment Organism
CsLAP1
-
 Clonorchis sinensis
CsLAP2
-
 Clonorchis sinensis
leucine aminopeptidase
-
 Clonorchis sinensis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
20
-
 assay at Clonorchis sinensis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.01
-
 L-Val-4-nitroanilide pH 8, 20°C Clonorchis sinensis
0.01
-
 L-Ile-4-nitroanilide pH 8, 20°C Clonorchis sinensis
0.01
-
 L-Thr-4-nitroanilide pH 8, 20°C Clonorchis sinensis
0.02
-
 L-Pro-4-nitroanilide pH 8, 20°C Clonorchis sinensis
0.02
-
 L-Thr-4-nitroanilide pH 8, 20°C Clonorchis sinensis
0.04
-
 L-Phe-4-nitroanilide pH 8, 20°C Clonorchis sinensis
0.05
-
 L-Lys-4-nitroanilide pH 8, 20°C Clonorchis sinensis
0.05
-
 L-Phe-4-nitroanilide pH 8, 20°C Clonorchis sinensis
0.07
-
 L-Lys-4-nitroanilide pH 8, 20°C Clonorchis sinensis
0.12
-
 L-Ala-4-nitroanilide pH 8, 20°C Clonorchis sinensis
0.18
-
 L-Met-4-nitroanilide pH 8, 20°C Clonorchis sinensis
0.2
-
 L-Met-4-nitroanilide pH 8, 20°C Clonorchis sinensis
0.23
-
 L-Leu-4-nitroanilide pH 8, 20°C Clonorchis sinensis
0.27
-
 L-Leu-4-nitroanilide pH 8, 20°C Clonorchis sinensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Clonorchis sinensis

Expression

Organism Comment Expression
Clonorchis sinensis expression at transcription levels increases gradually in accordance with the maturation of the parasite up

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.027
-
 L-Val-4-nitroanilide pH 8, 20°C Clonorchis sinensis
0.038
-
 L-Ile-4-nitroanilide pH 8, 20°C Clonorchis sinensis
0.049
-
 L-Thr-4-nitroanilide pH 8, 20°C Clonorchis sinensis
0.083
-
 L-Pro-4-nitroanilide pH 8, 20°C Clonorchis sinensis
0.092
-
 L-Pro-4-nitroanilide pH 8, 20°C Clonorchis sinensis
0.1
-
 L-Thr-4-nitroanilide pH 8, 20°C Clonorchis sinensis
0.44
-
 L-Phe-4-nitroanilide pH 8, 20°C Clonorchis sinensis
0.53
-
 L-Lys-4-nitroanilide pH 8, 20°C Clonorchis sinensis
0.59
-
 L-Ala-4-nitroanilide pH 8, 20°C Clonorchis sinensis
0.59
-
 L-Phe-4-nitroanilide pH 8, 20°C Clonorchis sinensis
0.6
-
 L-Ala-4-nitroanilide pH 8, 20°C Clonorchis sinensis
0.68
-
 L-Lys-4-nitroanilide pH 8, 20°C Clonorchis sinensis
3
-
 L-Met-4-nitroanilide pH 8, 20°C Clonorchis sinensis
3.7
-
 L-Met-4-nitroanilide pH 8, 20°C Clonorchis sinensis
6.3
-
 L-Leu-4-nitroanilide pH 8, 20°C Clonorchis sinensis
7.7
-
 L-Leu-4-nitroanilide pH 8, 20°C Clonorchis sinensis