Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Pyrococcus horikoshii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
bestatin | 0.05 mM 35.6% inhibition | Pyrococcus horikoshii | |
EDTA | 2 mM, 80% inhibition | Pyrococcus horikoshii | |
Mn2+ | 0.5 mM, around 50% inhibition | Pyrococcus horikoshii | |
additional information | insensitive to chamostatin, E-64, leupeptin, pepstatin, aprotinin and puromycin | Pyrococcus horikoshii | |
phosphoramidon | 1 mM, 25% inhibition | Pyrococcus horikoshii | |
Zn2+ | 0.5 mM, around 50% inhibition | Pyrococcus horikoshii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | optimally stimulates at 0.5-10 mM | Pyrococcus horikoshii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
475000 | - |
gel filtration | Pyrococcus horikoshii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus horikoshii | O59196 | - |
- |
Pyrococcus horikoshii OT-3 | O59196 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Pyrococcus horikoshii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-alanine 4-nitroanilide + H2O | 10.8% of the activity compared to Leu-4-nitroanilide | Pyrococcus horikoshii | L-alanine + 4-nitroaniline | - |
? | |
L-alanine 4-nitroanilide + H2O | 10.8% of the activity compared to Leu-4-nitroanilide | Pyrococcus horikoshii OT-3 | L-alanine + 4-nitroaniline | - |
? | |
L-isoleucine 4-nitroanilide + H2O | 14.3% of the activity compared to Leu-4-nitroanilide | Pyrococcus horikoshii | L-isoleucine + 4-nitroaniline | - |
? | |
L-isoleucine 4-nitroanilide + H2O | 14.3% of the activity compared to Leu-4-nitroanilide | Pyrococcus horikoshii OT-3 | L-isoleucine + 4-nitroaniline | - |
? | |
L-leucine 4-nitroanilide + H2O | - |
Pyrococcus horikoshii | L-leucine + 4-nitroaniline | - |
? | |
L-leucine 4-nitroanilide + H2O | - |
Pyrococcus horikoshii OT-3 | L-leucine + 4-nitroaniline | - |
? | |
L-methionine 4-nitroanilide + H2O | 26.3% of the activity compared to Leu-4-nitroanilide | Pyrococcus horikoshii | L-methionine + 4-nitroaniline | - |
? | |
L-methionine 4-nitroanilide + H2O | 26.3% of the activity compared to Leu-4-nitroanilide | Pyrococcus horikoshii OT-3 | L-methionine + 4-nitroaniline | - |
? | |
additional information | when assayed with short chromogenic peptides, PhTET2 is an aminopeptidase, with a clear preference for leucine as the N-terminal amino acid. The enzyme can cleave moderately long polypeptide substrates of various compositions in a fairly unspecific manner. The hydrolytic mechanism is nonprocessive. The enzyme has neither carboxypeptidase nor endoproteolytic activities, and it is devoid of N-terminal deblocking activity | Pyrococcus horikoshii | ? | - |
? | |
additional information | when assayed with short chromogenic peptides, PhTET2 is an aminopeptidase, with a clear preference for leucine as the N-terminal amino acid. The enzyme can cleave moderately long polypeptide substrates of various compositions in a fairly unspecific manner. The hydrolytic mechanism is nonprocessive. The enzyme has neither carboxypeptidase nor endoproteolytic activities, and it is devoid of N-terminal deblocking activity | Pyrococcus horikoshii OT-3 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dodecamer | - |
Pyrococcus horikoshii |
Synonyms | Comment | Organism |
---|---|---|
PH1527 | - |
Pyrococcus horikoshii |
PhTET2 | - |
Pyrococcus horikoshii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
100 | - |
- |
Pyrococcus horikoshii |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
half-life: 10.03 h | Pyrococcus horikoshii |
90 | - |
half-life: 3.0 h | Pyrococcus horikoshii |
95 | - |
half-life: 59.4 min | Pyrococcus horikoshii |
100 | - |
half-life: 24.8 min | Pyrococcus horikoshii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
PIPES buffer | Pyrococcus horikoshii |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | pH 7.0: about 60% of maximal activity, pH 9.0: about 65% of maximal activity | Pyrococcus horikoshii |