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Literature summary for 3.4.11.1 extracted from
- Purification and characterization of a leucine aminopeptidase from the bovine filarial parasite Setaria cervi (2008), Acta Trop., 106, 1-8.
Application
| Application | Comment | Organism |
|---|---|---|
| medicine | serodiagnostic marker against active filarial infection | Setaria cervi |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,10-phenanthroline | 5.0 mM, 55% inhibition | Setaria cervi |  |
| amastatin | 0.01 mM, 81% inhibition | Setaria cervi | |
| Ba2+ | 1 mM, 74% inhibition | Setaria cervi | |
| bestatin | 0.001 mM, 58% inhibition | Setaria cervi | |
| Cd2+ | 1 mM, 96% inhibition | Setaria cervi | |
| Cu2+ | 1 mM, 75% inhibition | Setaria cervi | |
| EDTA | 10 mM, 93% inhibition | Setaria cervi | |
| Fe2+ | 1 mM, 73% inhibition | Setaria cervi | |
| glycine | - |
Setaria cervi | |
| L-glutamine | - |
Setaria cervi | |
| L-leucine | - |
Setaria cervi | |
| Ni2+ | 1 mM, 55% inhibition | Setaria cervi | |
| Pb2+ | 1 mM, 60% inhibition | Setaria cervi | |
| Zn2+ | 1 mM, 56% inhibition | Setaria cervi | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 8.8 | - |
Leu-4-nitroanilide | pH 8.0, 37°C | Setaria cervi | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | 1 mM, 5.3fold activation | Setaria cervi | |
| Mg2+ | 1 mM, 4.7fold activation | Setaria cervi | |
| Mn2+ | 1 mM, 3.9fold activation | Setaria cervi | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 54000 | - |
6 * 54000, SDS-PAGE | Setaria cervi |
| 321000 | - |
gel filtration | Setaria cervi |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Setaria cervi | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Setaria cervi |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| adult | - |
Setaria cervi | - |
| microfilarial stage | - |
Setaria cervi | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 18.2 | - |
- |
Setaria cervi |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Leu-4-nitroanilide + H2O | - |
Setaria cervi | L-leucine + 4-nitroaniline | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| hexamer | 6 * 54000, SDS-PAGE | Setaria cervi |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
- |
Setaria cervi |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | 60 | 37°C: maximum, 60°C: 6.15% of maximal activity | Setaria cervi |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
completely denatured at 70°C and above | Setaria cervi |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
- |
Setaria cervi |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 8 | 9.5 | pH 8.0: about 25% of maximal activity, pH 9.5: about 10% of maximal activity | Setaria cervi |
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