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Literature summary for 3.4.11.1 extracted from

  • Nampoothiri, K.M.; Nagy, V.; Kovacs, K.; Szakacs, G.; Pandey, A.
    L-Leucine aminopeptidase production by filamentous Aspergillus fungi (2005), Lett. Appl. Microbiol., 41, 498-504.
    View publication on PubMed
Search for more literature for 3.4.11.1

Inhibitors

Inhibitors Comment Organism Structure
Al3+ 98% inhibition at 2 mM, complete inhibition at 10 mM Aspergillus oryzae
Al3+ strong inhibition Aspergillus sojae
Ca2+ 70% inhibition at 2 mM Aspergillus oryzae
Ca2+
-
 Aspergillus sojae
Cd2+ 80% inhibition at 2 mM Aspergillus oryzae
Cd2+
-
 Aspergillus sojae
Co2+ 39% activation at 2 mM, 40% inhibition at 10 mM Aspergillus oryzae
Co2+ activates at 2 mM, inhibits at 10 mM and above Aspergillus sojae
Cu2+ 86% inhibition at 2 mM, complete inhibition at 10 mM Aspergillus oryzae
Cu2+ strong inhibition Aspergillus sojae
Fe2+ 15% activation at 2 mM, 55% inhibition at 10 mM Aspergillus oryzae
Fe2+
-
 Aspergillus sojae
Mg2+ 50% inhibition at 2 mM Aspergillus oryzae
Mg2+
-
 Aspergillus sojae
Mn2+ 60% inhibition at 2 mM Aspergillus oryzae
Mn2+
-
 Aspergillus sojae
additional information inhibition of enzymes from strains NRRL 6271 and NRRL 1988 by different metal ions at different concentrations, overview Aspergillus sojae
Zn2+ 26% inhibition at 2 mM, 86% at 10 mM, and 95% at 20 mM Aspergillus oryzae
Zn2+
-
 Aspergillus sojae

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ 39% activation at 2 mM, 40% inhibition at 10 mM Aspergillus oryzae
Co2+ activates at 2 mM, inhibits at 10 mM and above Aspergillus sojae
Fe2+ 15% activation at 2 mM, 55% inhibition at 10 mM Aspergillus oryzae
K+ activates at 10-20 mM Aspergillus sojae
additional information no effect by K+ and Na+ Aspergillus oryzae
Na+ activates at 10-20 mM Aspergillus sojae

Organism

Organism UniProt Comment Textmining
Aspergillus oryzae
-
 other strains show low enzyme activity
-
Aspergillus oryzae NRRL 6270
-
 other strains show low enzyme activity
-
Aspergillus sojae
-
 strains NRRL 1988 and NRRL 6271, other strains show low enzyme activity
-

Purification (Commentary)

Purification (Comment) Organism
high LAP production in strain NRRL 6270, solid-state fermentation, process optimization, overview Aspergillus oryzae
high LAP production in strains NRRL 1988 and NRRL 6271, solid-state fermentation, process optimization, overview Aspergillus sojae

Source Tissue

Source Tissue Comment Organism Textmining
additional information optimization of filamentous culture conditions Aspergillus oryzae
-
additional information optimization of filamentous culture conditions Aspergillus sojae
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Aspergillus oryzae
additional information
-
-
 Aspergillus sojae

Synonyms

Synonyms Comment Organism
L-leucine aminopeptidase
-
 Aspergillus oryzae
L-leucine aminopeptidase
-
 Aspergillus sojae
LAP
-
 Aspergillus oryzae
LAP
-
 Aspergillus sojae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65 70 at pH 8.5 Aspergillus oryzae
65 70 strain NRRL 6271, at pH 8.5 Aspergillus sojae
75
-
 strain NRRL 1988, at pH 8.5 Aspergillus sojae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 at 50°C Aspergillus oryzae
8.5 9 at 50°C Aspergillus sojae