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Literature summary for 3.4.11.1 extracted from
- Leucyl aminopeptidase (plant) (2004), Handbook of Proteolytic Enzymes (2nd Edition), 1, 901-905.
No PubMed abstract available
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | wounding induces LAP-A | Solanum tuberosum | |
| additional information | wounding induces LAP-A, which is also induced by salt- and water-stress, and pathogen attack | Solanum lycopersicum |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| DNA sequence determination, overexpression of a LAP fusion protein in Escherichia coli | Arabidopsis thaliana |
| DNA sequence determination, overexpression of mature LAP-A, His6-tagged LAP-A and pre-LAP-A in Escherichia coli | Solanum lycopersicum |
| DNA sequence determination, overexpression of pre-LAP protein in Escherichia coli | Solanum tuberosum |
| overexpression in Escherichia coli | Phaseolus vulgaris |
| overexpression in Escherichia coli | Hordeum vulgare |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D347E | site-directed mutagenesis, inactive mutant | Solanum lycopersicum |
| D347R | site-directed mutagenesis, reduced activity | Solanum lycopersicum |
| E429D | site-directed mutagenesis, 95% activity compared to wild-type enzyme | Solanum lycopersicum |
| E429X | site-directed mutagenesis, inactive mutants, overview | Solanum lycopersicum |
| K354X | site-directed mutagenesis, inactive mutants, overview | Solanum lycopersicum |
| R431K | site-directed mutagenesis, 4fold reduced activity | Solanum lycopersicum |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| amastatin | inhibition of LAP-A1 | Solanum lycopersicum |  |
| bestatin | transition-state analogue, complete inhibition | Phaseolus vulgaris | |
| bestatin | inhibition of LAP-A1 | Solanum lycopersicum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.5 | - |
L-Leu-4-nitroanilide | - |
Arabidopsis thaliana | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | activates | Hordeum vulgare | |
| Mg2+ | activates | Spinacia oleracea | |
| Mg2+ | activates | Brassica sp. | |
| Mg2+ | activates, LAP-A1 | Solanum lycopersicum | |
| Mn2+ | activates | Hordeum vulgare | |
| Mn2+ | activates | Spinacia oleracea | |
| Mn2+ | activates | Brassica sp. | |
| Mn2+ | activates, LAP-A1 | Solanum lycopersicum | |
| additional information | metallopeptidase | Phaseolus vulgaris | |
| additional information | metallopeptidase | Hordeum vulgare | |
| additional information | metallopeptidase | Spinacia oleracea | |
| additional information | metallopeptidase | Solanum tuberosum | |
| additional information | metallopeptidase | Arabidopsis thaliana | |
| additional information | metallopeptidase | Brassica sp. | |
| Zn2+ | metallopeptidase, bound by residues Asp347 and Glu429 | Solanum lycopersicum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 55000 | - |
6 * 55000, wound-induced LAP-A | Solanum lycopersicum |
| 55000 | - |
x * 55000, LAP-A | Solanum tuberosum |
| 58000 | - |
x * 58000 + x * 66000 | Phaseolus vulgaris |
| 66000 | - |
x * 58000 + x * 66000 | Phaseolus vulgaris |
| 320000 | 360000 | - |
Phaseolus vulgaris |
| 320000 | 360000 | - |
Hordeum vulgare |
| 320000 | 360000 | - |
Spinacia oleracea |
| 320000 | 360000 | - |
Solanum tuberosum |
| 320000 | 360000 | - |
Arabidopsis thaliana |
| 320000 | 360000 | - |
Solanum lycopersicum |
| 320000 | 360000 | - |
Brassica sp. |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arabidopsis thaliana | - |
- |
- |
| Brassica sp. | - |
cabbage | - |
| Hordeum vulgare | - |
- |
- |
| Phaseolus vulgaris | - |
kidney bean | - |
| Solanum lycopersicum | - |
LAP-A, encoded by genes LAP-A1 and LAP-A2 | - |
| Solanum tuberosum | - |
- |
- |
| Spinacia oleracea | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| proteolytic modification | the pre-protein is processed to its mature form | Solanum tuberosum |
| proteolytic modification | the pre-protein is processed to its mature form | Solanum lycopersicum |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme, and recombinant enzyme from Escherichia coli | Phaseolus vulgaris |
| native enzyme, and recombinant enzyme from Escherichia coli | Hordeum vulgare |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Solanum lycopersicum | - |
| seed | germinated | Hordeum vulgare | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-Ala-4-nitroanilide + H2O | - |
Solanum lycopersicum | L-Ala + 4-nitroaniline | - |
? | |
| L-Arg-4-nitroanilide + H2O | - |
Solanum lycopersicum | L-Arg + 4-nitroaniline | - |
? | |
| L-Arg-4-nitroanilide + H2O | pre-LAP | Solanum tuberosum | L-Arg + 4-nitroaniline | - |
? | |
| L-Ile-4-nitroanilide + H2O | - |
Solanum lycopersicum | L-Ile + 4-nitroaniline | - |
? | |
| L-Leu-4-nitroanilide + H2O | - |
Arabidopsis thaliana | L-Leu + 4-nitroaniline | - |
? | |
| L-Leu-4-nitroanilide + H2O | - |
Solanum lycopersicum | L-Leu + 4-nitroaniline | - |
? | |
| L-Leu-4-nitroanilide + H2O | pre-LAP | Solanum tuberosum | L-Leu + 4-nitroaniline | - |
? | |
| L-Met-4-nitroanilide + H2O | - |
Solanum lycopersicum | L-Met + 4-nitroaniline | - |
? | |
| L-Met-4-nitroanilide + H2O | pre-LAP | Solanum tuberosum | L-Met + 4-nitroaniline | - |
? | |
| L-Val-4-nitroanilide + H2O | - |
Solanum lycopersicum | L-Val + 4-nitroaniline | - |
? | |
| Leu-systemin + H2O | LAP-A1, only in vitro | Solanum lycopersicum | Leu + systemin | - |
? | |
| additional information | no activity towards substrates with Asp or Glu at position P1, Arg431 and Lys354 of LAP-A1 are involved in catalysis | Solanum lycopersicum | ? | - |
? | |
| additional information | the enzyme hydrolyses with Leu or Met in the P1 position | Hordeum vulgare | ? | - |
? | |
| additional information | the enzyme hydrolyses with Leu or Met in the P1 position, the residues at P1', P2'a nd P3' positions influence the activity | Phaseolus vulgaris | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 55000, LAP-A | Solanum tuberosum |
| hexamer | 6 * 55000, wound-induced LAP-A | Solanum lycopersicum |
| hexamer | x * 58000 + x * 66000 | Phaseolus vulgaris |
| multimer | - |
Hordeum vulgare |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| LAP | - |
Phaseolus vulgaris |
| LAP | - |
Hordeum vulgare |
| LAP-A | - |
Solanum tuberosum |
| LAP-A | - |
Solanum lycopersicum |
| leucyl aminopeptidase (plant) | - |
Phaseolus vulgaris |
| leucyl aminopeptidase (plant) | - |
Hordeum vulgare |
| leucyl aminopeptidase (plant) | - |
Spinacia oleracea |
| leucyl aminopeptidase (plant) | - |
Solanum tuberosum |
| leucyl aminopeptidase (plant) | - |
Arabidopsis thaliana |
| leucyl aminopeptidase (plant) | - |
Solanum lycopersicum |
| leucyl aminopeptidase (plant) | - |
Brassica sp. |
| leucylpeptidase | - |
Hordeum vulgare |
| leucylpeptidase | - |
Spinacia oleracea |
| leucylpeptidase | - |
Brassica sp. |
| More | the enzyme belongs to peptidase family M17 | Phaseolus vulgaris |
| More | the enzyme belongs to peptidase family M17 | Hordeum vulgare |
| More | the enzyme belongs to peptidase family M17 | Spinacia oleracea |
| More | the enzyme belongs to peptidase family M17 | Solanum tuberosum |
| More | the enzyme belongs to peptidase family M17 | Arabidopsis thaliana |
| More | the enzyme belongs to peptidase family M17 | Solanum lycopersicum |
| More | the enzyme belongs to peptidase family M17 | Brassica sp. |
| peptidase II | - |
Hordeum vulgare |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
the enzyme is thermostable | Phaseolus vulgaris |
| additional information | - |
the enzyme is thermostable | Hordeum vulgare |
| additional information | - |
the enzyme is thermostable | Spinacia oleracea |
| additional information | - |
the enzyme is thermostable | Solanum tuberosum |
| additional information | - |
the enzyme is thermostable | Arabidopsis thaliana |
| additional information | - |
the enzyme is thermostable | Solanum lycopersicum |
| additional information | - |
the enzyme is thermostable | Brassica sp. |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | 9.5 | - |
Phaseolus vulgaris |
| 8.5 | 9.5 | - |
Hordeum vulgare |
| 8.5 | 9.5 | - |
Spinacia oleracea |
| 8.5 | 9.5 | - |
Solanum tuberosum |
| 8.5 | 9.5 | - |
Arabidopsis thaliana |
| 8.5 | 9.5 | - |
Solanum lycopersicum |
| 8.5 | 9.5 | - |
Brassica sp. |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0000015 | - |
bestatin | - |
Phaseolus vulgaris | |
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