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Literature summary for 3.4.11.1 extracted from
- Purification and some properties of an aminopeptidase from the seeds of Cannabis sativa (2000), Biosci. Biotechnol. Biochem., 64, 1055-1057.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 4-chloromercuriphenylsulfonic acid | strong inhibition | Cannabis sativa |  |
| Co2+ | slight inhibition | Cannabis sativa | |
| iodoacetic acid | slight inhibition | Cannabis sativa | |
| Mn2+ | slight inhibition | Cannabis sativa | |
| additional information | no inhibition by bestatin, EDTA, EGTA, PMSF, and DFP | Cannabis sativa | |
| Zn2+ | strong inhibition | Cannabis sativa | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.194 | - |
Pro-4-nitroanilide | pH 7.0, 37°C | Cannabis sativa | |
| 0.248 | - |
Leu-4-nitroanilide | pH 7.0, 37°C | Cannabis sativa | |
| 8.78 | - |
Ala-4-nitroanilide | pH 7.0, 37°C | Cannabis sativa | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 78000 | - |
x * 78000, SDS-PAGE | Cannabis sativa |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cannabis sativa | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme 474fold from seeds by ammonium sulfate fractionation, anion exchange chromatography, gel filtration, adsorption chromatography, and two steps of leucine affinity chromatography to homogeneity | Cannabis sativa |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| seed | - |
Cannabis sativa | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.18 | - |
purified enzyme | Cannabis sativa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Ala-4-nitroanilide + H2O | 42% of the activity with Leu-4-nitroanilide | Cannabis sativa | Ala + 4-nitroaniline | - |
? | |
| Leu-4-nitroanilide + H2O | best substrate | Cannabis sativa | Leu + 4-nitroaniline | - |
? | |
| Leu-Gly-Tyr-Leu + H2O | synthetic peptide substrate, successive release of terminal amino acids with descending activity | Cannabis sativa | Leu + Gly + Tyr + Leu | - |
? | |
| additional information | the enzyme acts as aminopeptidase and endopeptidase and might be a cysteine type peptidase, no activity with N-blocked substrate, Gly-4-nitroanilide, Arg-4-nitroanilide, and Lys-4-nitroanilide are poor substrates, Glu-4-nitroanilide is no substrate | Cannabis sativa | ? | - |
? | |
| Pro-4-nitroanilide + H2O | 30% of the activity with Leu-4-nitroanilide | Cannabis sativa | Pro + 4-nitroaniline | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 78000, SDS-PAGE | Cannabis sativa |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| HSA | - |
Cannabis sativa |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Cannabis sativa |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
60 min, purified enzyme, fairly stable up to | Cannabis sativa |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
about | Cannabis sativa |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 8 | at least 80% of maximal activity at pH 6.0 and pH 8.0 | Cannabis sativa |
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