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Literature summary for 3.4.11.1 extracted from

  • Gu, Y.Q.; Walling, L.L.
    Specificity of the wound-induced leucine aminopeptidase (LAP-A) of tomato. Activity on dipeptide and tripeptide substrates (2000), Eur. J. Biochem., 267, 1178-1187.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
overexpression of free and His-tagged enzyme in peptidase A-deficient Escherichia coli strain DS957 xerB:Tn5 Solanum lycopersicum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4
-
Arg-Leu His-tagged recombinant enzyme, 37°C Solanum lycopersicum
1.5
-
Leu-Gly 37°C Sus scrofa
1.9
-
Leu-Gly-Gly His-tagged recombinant enzyme, 37°C Solanum lycopersicum
2.2
-
Leu-Gly free recombinant enzyme, 37°C Solanum lycopersicum
2.9
-
Leu-Leu His-tagged recombinant enzyme, 37°C Solanum lycopersicum
3.2
-
Leu-Gly His-tagged recombinant enzyme, 37°C Solanum lycopersicum
3.3
-
Arg-Gly-Asp His-tagged recombinant enzyme, 37°C Solanum lycopersicum
4.5
-
Arg-Gly His-tagged recombinant enzyme, 37°C Solanum lycopersicum
8.6
-
Gly-Leu His-tagged recombinant enzyme, 37°C Solanum lycopersicum
9.6
-
Arg-Gly-Phe His-tagged recombinant enzyme, 37°C Solanum lycopersicum
11.2
-
Gly-Gly His-tagged recombinant enzyme, 37°C Solanum lycopersicum

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ activates Escherichia coli
Mn2+ activates Sus scrofa
Mn2+ activates Solanum lycopersicum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
53500
-
x * 53500, SDS-PAGE Escherichia coli
54000
-
x * 54000, SDS-PAGE Sus scrofa
54868
-
x * 54868, free enzyme, sequence calculation, up to 6 subunits form the oligomer, oligomeric form is required for activity Solanum lycopersicum

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Solanum lycopersicum
-
wound-induced enzyme
-
Sus scrofa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant free and His-tagged enzymes from Escherichia coli, to near homogeneity Solanum lycopersicum

Reaction

Reaction Comment Organism Reaction ID
release of an N-terminal amino acid, Xaa-/-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolysed, but rates on arylamides are exceedingly low exoprotease, preference for N-terminal Leu, Met and Arg residues, low activity when Xaa is Gly or Trp, no activity when Xaa is Asp Sus scrofa
release of an N-terminal amino acid, Xaa-/-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolysed, but rates on arylamides are exceedingly low exoprotease, preference for N-terminal Leu, Met and Arg residues, low activity when Xaa is Gly or Tyr, no activity when Xaa is Asp Solanum lycopersicum
release of an N-terminal amino acid, Xaa-/-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolysed, but rates on arylamides are exceedingly low exoprotease, preference for N-terminal Leu, Met and Arg residues, low activity when Xaa is Gly, no activity when Xaa is Asp Escherichia coli

Source Tissue

Source Tissue Comment Organism Textmining
commercial preparation
-
Sus scrofa
-
kidney
-
Sus scrofa
-
leaf enzyme accumulation in wounded leaves Solanum lycopersicum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.3
-
purified enzyme, substrate Arg-Gly-Arg Sus scrofa
13
-
purified recombinant enzyme, substrate Arg-Gly-Arg Solanum lycopersicum
19
-
purified enzyme, substrate Arg-Gly-Ser Sus scrofa
21
-
purified enzyme, substrate Arg-Gly-Gly Sus scrofa
23.7
-
purified enzyme, substrate Arg-Gly-Leu or Arg-Gly-Phe Sus scrofa
39.9
-
purified recombinant enzyme, substrate Arg-Gly-Asp Solanum lycopersicum
47.7
-
purified recombinant enzyme, substrate Arg-Gly-Ser Solanum lycopersicum
53.2
-
purified recombinant enzyme, substrate Arg-Gly-Phe Solanum lycopersicum
53.5
-
purified enzyme, substrate Leu-Gly-Gly Sus scrofa
57.1
-
purified enzyme, substrate Arg-Gly Sus scrofa
68.7
-
purified enzyme, substrate Arg-Gly-Arg Escherichia coli
69.3
-
purified enzyme, substrate Arg-Gly-Asp Sus scrofa
70.9
-
purified recombinant enzyme, substrate Arg-Gly-Leu Solanum lycopersicum
77.5
-
purified recombinant enzyme, substrate Arg-Gly-Gly Solanum lycopersicum
130.4
-
purified recombinant enzyme, substrate Leu-Gly Solanum lycopersicum
139
-
purified recombinant enzyme, substrate Arg-Gly Solanum lycopersicum
170.3
-
purified enzyme, substrate Leu-Gly Sus scrofa
196.7
-
purified enzyme, substrate Arg-Gly-Ser Escherichia coli
241.6
-
purified recombinant enzyme, substrate Leu-Gly-Gly Solanum lycopersicum
300.1
-
purified enzyme, substrate Arg-Gly-Gly Escherichia coli
318
-
purified enzyme, substrate Leu-Gly Escherichia coli
355.1
-
purified enzyme, substrate Arg-Gly-Phe Escherichia coli
396.3
-
purified enzyme, substrate Arg-Gly-Leu Escherichia coli
443.1
-
purified enzyme, substrate Arg-Gly Escherichia coli
604.7
-
purified enzyme, substrate Leu-Gly-Gly Escherichia coli
790.5
-
purified enzyme, substrate Arg-Gly-Asp Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Ala-Gly + H2O
-
Solanum lycopersicum Ala + Gly
-
?
Ala-Gly + H2O low activity Escherichia coli Ala + Gly
-
?
Ala-Gly + H2O low activity Sus scrofa Ala + Gly
-
?
Ala-Leu + H2O high activity Escherichia coli Ala + Leu
-
?
Ala-Leu + H2O high activity Sus scrofa Ala + Leu
-
?
Ala-Leu + H2O high activity Solanum lycopersicum Ala + Leu
-
?
Arg-Ala + H2O
-
Escherichia coli Arg + Ala
-
?
Arg-Ala + H2O
-
Sus scrofa Arg + Ala
-
?
Arg-Ala + H2O
-
Solanum lycopersicum Arg + Ala
-
?
Arg-Glu + H2O low activity Escherichia coli Arg + Glu
-
?
Arg-Glu + H2O low activity Sus scrofa Arg + Glu
-
?
Arg-Glu + H2O low activity Solanum lycopersicum Arg + Glu
-
?
Arg-Gly + H2O
-
Escherichia coli Arg + Gly
-
?
Arg-Gly + H2O
-
Sus scrofa Arg + Gly
-
?
Arg-Gly + H2O
-
Solanum lycopersicum Arg + Gly
-
?
Arg-Gly + H2O low activity Escherichia coli Arg + Gly
-
?
Arg-Gly + H2O low activity Sus scrofa Arg + Gly
-
?
Arg-Gly + H2O low activity Solanum lycopersicum Arg + Gly
-
?
Arg-Gly-Arg + H2O low activity Escherichia coli Arg + Gly-Arg
-
?
Arg-Gly-Arg + H2O low activity Sus scrofa Arg + Gly-Arg
-
?
Arg-Gly-Arg + H2O low activity Solanum lycopersicum Arg + Gly-Arg
-
?
Arg-Gly-Asp + H2O
-
Escherichia coli Arg + Gly-Asp
-
?
Arg-Gly-Asp + H2O
-
Sus scrofa Arg + Gly-Asp
-
?
Arg-Gly-Asp + H2O
-
Solanum lycopersicum Arg + Gly-Asp
-
?
Arg-Gly-Gly + H2O
-
Escherichia coli Arg + Gly-Gly
-
?
Arg-Gly-Gly + H2O
-
Sus scrofa Arg + Gly-Gly
-
?
Arg-Gly-Gly + H2O
-
Solanum lycopersicum Arg + Gly-Gly
-
?
Arg-Gly-Leu + H2O
-
Escherichia coli Arg + Gly-Leu
-
?
Arg-Gly-Leu + H2O
-
Sus scrofa Arg + Gly-Leu
-
?
Arg-Gly-Leu + H2O
-
Solanum lycopersicum Arg + Gly-Leu
-
?
Arg-Gly-Phe + H2O
-
Escherichia coli Arg + Gly-Phe
-
?
Arg-Gly-Phe + H2O
-
Sus scrofa Arg + Gly-Phe
-
?
Arg-Gly-Phe + H2O
-
Solanum lycopersicum Arg + Gly-Phe
-
?
Arg-Gly-Ser + H2O
-
Escherichia coli Arg + Gly-Ser
-
?
Arg-Gly-Ser + H2O
-
Sus scrofa Arg + Gly-Ser
-
?
Arg-Gly-Ser + H2O
-
Solanum lycopersicum Arg + Gly-Ser
-
?
Arg-Ile + H2O
-
Escherichia coli Arg + Ile
-
?
Arg-Ile + H2O
-
Sus scrofa Arg + Ile
-
?
Arg-Ile + H2O
-
Solanum lycopersicum Arg + Ile
-
?
Arg-Leu + H2O high activity Escherichia coli Arg + Leu
-
?
Arg-Leu + H2O high activity Sus scrofa Arg + Leu
-
?
Arg-Leu + H2O high activity Solanum lycopersicum Arg + Leu
-
?
Arg-Phe + H2O
-
Escherichia coli Arg + Phe
-
?
Arg-Phe + H2O
-
Sus scrofa Arg + Phe
-
?
Arg-Phe + H2O
-
Solanum lycopersicum Arg + Phe
-
?
Arg-Val + H2O
-
Escherichia coli Arg + Val
-
?
Arg-Val + H2O
-
Sus scrofa Arg + Val
-
?
Arg-Val + H2O
-
Solanum lycopersicum Arg + Val
-
?
Cys-Gly + H2O low activity Escherichia coli Cys + Gly
-
?
Cys-Gly + H2O low activity Sus scrofa Cys + Gly
-
?
Gly-Leu + H2O low activity Escherichia coli Gly + Leu
-
?
Gly-Leu + H2O low activity Sus scrofa Gly + Leu
-
?
Gly-Leu + H2O low activity Solanum lycopersicum Gly + Leu
-
?
Gly-Phe + H2O
-
Sus scrofa Gly + Phe
-
?
Gly-Phe + H2O
-
Solanum lycopersicum Gly + Phe
-
?
Gly-Phe + H2O low activity Escherichia coli Gly + Phe
-
?
Gly-Trp + H2O low activity Escherichia coli Gly + Trp
-
?
Gly-Trp + H2O low activity Sus scrofa Gly + Trp
-
?
Gly-Tyr + H2O low activity Escherichia coli Gly + Tyr
-
?
Gly-Tyr + H2O low activity Sus scrofa Gly + Tyr
-
?
Gly-Tyr + H2O low activity Solanum lycopersicum Gly + Tyr
-
?
Gly-Val + H2O low activity Solanum lycopersicum Gly + Val
-
?
His-Gly + H2O
-
Escherichia coli His + Gly
-
?
His-Gly + H2O
-
Sus scrofa His + Gly
-
?
His-Gly + H2O
-
Solanum lycopersicum His + Gly
-
?
His-Leu + H2O
-
Sus scrofa His + Leu
-
?
His-Leu + H2O
-
Solanum lycopersicum His + Leu
-
?
His-Leu + H2O low activity Escherichia coli His + Leu
-
?
Leu-4-nitroanilide + H2O
-
Escherichia coli Leu + 4-nitroaniline
-
?
Leu-4-nitroanilide + H2O
-
Sus scrofa Leu + 4-nitroaniline
-
?
Leu-4-nitroanilide + H2O
-
Solanum lycopersicum Leu + 4-nitroaniline
-
?
Leu-Arg + H2O
-
Escherichia coli Leu + Arg
-
?
Leu-Arg + H2O
-
Sus scrofa Leu + Arg
-
?
Leu-Arg + H2O
-
Solanum lycopersicum Leu + Arg
-
?
Leu-Asn + H2O
-
Escherichia coli Leu + Asn
-
?
Leu-Asn + H2O
-
Sus scrofa Leu + Asn
-
?
Leu-Asn + H2O
-
Solanum lycopersicum Leu + Asn
-
?
Leu-Asp + H2O low activity Escherichia coli Leu + Asp
-
?
Leu-Asp + H2O low activity Sus scrofa Leu + Asp
-
?
Leu-Gly + H2O
-
Escherichia coli Leu + Gly
-
?
Leu-Gly + H2O
-
Sus scrofa Leu + Gly
-
?
Leu-Gly + H2O
-
Solanum lycopersicum Leu + Gly
-
?
Leu-Gly-Gly + H2O
-
Escherichia coli Leu + Gly-Gly
-
?
Leu-Gly-Gly + H2O
-
Sus scrofa Leu + Gly-Gly
-
?
Leu-Gly-Gly + H2O
-
Solanum lycopersicum Leu + Gly-Gly
-
?
Leu-Leu + H2O
-
Escherichia coli Leu + Leu
-
?
Leu-Leu + H2O high activity Sus scrofa Leu + Leu
-
?
Leu-Leu + H2O best dipeptide substrate Solanum lycopersicum Leu + Leu
-
?
Leu-Met + H2O
-
Escherichia coli Leu + Met
-
?
Leu-Met + H2O
-
Sus scrofa Leu + Met
-
?
Leu-Met + H2O
-
Solanum lycopersicum Leu + Met
-
?
Leu-Phe + H2O
-
Escherichia coli Leu + Phe
-
?
Leu-Phe + H2O
-
Sus scrofa Leu + Phe
-
?
Leu-Phe + H2O
-
Solanum lycopersicum Leu + Phe
-
?
Leu-Ser + H2O
-
Escherichia coli Leu + Ser
-
?
Leu-Ser + H2O
-
Sus scrofa Leu + Ser
-
?
Leu-Ser + H2O
-
Solanum lycopersicum Leu + Ser
-
?
Leu-Trp + H2O
-
Escherichia coli Leu + Trp
-
?
Leu-Trp + H2O
-
Sus scrofa Leu + Trp
-
?
Leu-Trp + H2O
-
Solanum lycopersicum Leu + Trp
-
?
Leu-Tyr + H2O
-
Escherichia coli Leu + Tyr
-
?
Leu-Tyr + H2O
-
Sus scrofa Leu + Tyr
-
?
Leu-Tyr + H2O
-
Solanum lycopersicum Leu + Tyr
-
?
Leu-Val + H2O
-
Escherichia coli Leu + Val
-
?
Leu-Val + H2O
-
Sus scrofa Leu + Val
-
?
Leu-Val + H2O
-
Solanum lycopersicum Leu + Val
-
?
Met-Leu + H2O
-
Escherichia coli Met + Leu
-
?
Met-Leu + H2O high activity Sus scrofa Met + Leu
-
?
Met-Leu + H2O high activity Solanum lycopersicum Met + Leu
-
?
additional information substrate specificity study, Asp-Leu, Pro-Gly, Gly-Gly, Cys-Gly, Ser-Gly, Gly-Trp, Gly-Pro, Gly-His, Gly-Lys and Lys-Gly are poor substrates, Asp-Gly, Leu-Asp, Arg-Lys, Arg-Asp, Gly-Asn, Gly-Gln, and Leu-Pro are no substrates Solanum lycopersicum ?
-
?
additional information substrate specificity study, Asp-Leu, Pro-Gly, Gly-Gly, Ser-Gly, Arg-Lys, Gly-Ala, Gly-Gln, Gly-His, Gly-Val and Lys-Gly are poor substrates, Asp-Gly, Arg-Asp, Gly-Arg, Gly-Asn, Gly-Lys, and Leu-Pro are no substrates Sus scrofa ?
-
?
additional information substrate specificity study, Asp-Leu, Pro-Gly, Lys-Gly, Ser-Gly, Arg-Lys, Arg-Asp, Gly-Ala, Gly-Val, Gly-Trp, Gly-Gln, Gly-Gly, Gly-Lys, Gly-His, and Leu-Pro are poor substrates, Gly-Asn, Asp-Gly, Gly-Arg, and Gly-Asn are no substrates Escherichia coli ?
-
?
Phe-Gly + H2O
-
Escherichia coli Phe + Gly
-
?
Phe-Gly + H2O
-
Sus scrofa Phe + Gly
-
?
Phe-Gly + H2O
-
Solanum lycopersicum Phe + Gly
-
?
Phe-Leu + H2O
-
Sus scrofa Phe + Leu
-
?
Phe-Leu + H2O
-
Solanum lycopersicum Phe + Leu
-
?
Phe-Leu + H2O high activity Escherichia coli Phe + Leu
-
?
Pro-Leu + H2O
-
Escherichia coli Pro + Leu
-
?
Pro-Leu + H2O
-
Sus scrofa Pro + Leu
-
?
Pro-Leu + H2O
-
Solanum lycopersicum Pro + Leu
-
?
Thr-Leu + H2O
-
Escherichia coli Thr + Leu
-
?
Thr-Leu + H2O
-
Sus scrofa Thr + Leu
-
?
Thr-Leu + H2O
-
Solanum lycopersicum Thr + Leu
-
?
Trp-Leu + H2O
-
Solanum lycopersicum Trp + Leu
-
?
Trp-Leu + H2O low activity Escherichia coli Trp + Leu
-
?
Trp-Leu + H2O low activity Sus scrofa Trp + Leu
-
?
Tyr-Gly + H2O
-
Escherichia coli Tyr + Gly
-
?
Tyr-Gly + H2O
-
Sus scrofa Tyr + Gly
-
?
Tyr-Gly + H2O low activity Solanum lycopersicum Tyr + Gly
-
?
Tyr-Leu + H2O
-
Escherichia coli Tyr + Leu
-
?
Tyr-Leu + H2O
-
Sus scrofa Tyr + Leu
-
?
Tyr-Leu + H2O low activity Solanum lycopersicum Tyr + Leu
-
?
Val-Gly + H2O
-
Escherichia coli Val + Gly
-
?
Val-Gly + H2O
-
Sus scrofa Val + Gly
-
?
Val-Gly + H2O
-
Solanum lycopersicum Val + Gly
-
?
Val-Leu + H2O high activity Escherichia coli Val + Leu
-
?
Val-Leu + H2O high activity Sus scrofa Val + Leu
-
?
Val-Leu + H2O high activity Solanum lycopersicum Val + Leu
-
?

Subunits

Subunits Comment Organism
? x * 54000, SDS-PAGE Sus scrofa
? x * 53500, SDS-PAGE Escherichia coli
oligomer x * 54868, free enzyme, sequence calculation, up to 6 subunits form the oligomer, oligomeric form is required for activity Solanum lycopersicum

Synonyms

Synonyms Comment Organism
LAP
-
Escherichia coli
LAP
-
Sus scrofa
LAP-A
-
Solanum lycopersicum
leucine aminopeptidase
-
Escherichia coli
leucine aminopeptidase
-
Sus scrofa
leucine aminopeptidase
-
Solanum lycopersicum
PepA peptidase A
-
Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Solanum lycopersicum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3780
-
Arg-Gly-Asp His-tagged recombinant enzyme, 37°C Solanum lycopersicum
3781
-
Arg-Gly-Asp His-tagged recombinant enzyme, 37°C Solanum lycopersicum
5140
-
Gly-Leu His-tagged recombinant enzyme, 37°C Solanum lycopersicum
5141
-
Gly-Leu His-tagged recombinant enzyme, 37°C Solanum lycopersicum
6070
-
Arg-Gly-Phe His-tagged recombinant enzyme, 37°C Solanum lycopersicum
6073
-
Arg-Gly-Phe His-tagged recombinant enzyme, 37°C Solanum lycopersicum
10860
-
Gly-Gly His-tagged recombinant enzyme, 37°C Solanum lycopersicum
10900
-
Gly-Gly His-tagged recombinant enzyme, 37°C Solanum lycopersicum
13500
-
Arg-Gly His-tagged recombinant enzyme, 37°C Solanum lycopersicum
13520
-
Arg-Gly His-tagged recombinant enzyme, 37°C Solanum lycopersicum
13960
-
Leu-Gly-Gly His-tagged recombinant enzyme, 37°C Solanum lycopersicum
14000
-
Leu-Gly-Gly His-tagged recombinant enzyme, 37°C Solanum lycopersicum
14100
-
Leu-Gly His-tagged recombinant enzyme, 37°C Solanum lycopersicum
14400
-
Leu-Gly free recombinant enzyme, 37°C Solanum lycopersicum
14440
-
Leu-Gly free recombinant enzyme, 37°C Solanum lycopersicum
19380
-
Arg-Leu His-tagged recombinant enzyme, 37°C Solanum lycopersicum
19400
-
Arg-Leu His-tagged recombinant enzyme, 37°C Solanum lycopersicum
34490
-
Leu-Leu His-tagged recombinant enzyme, 37°C Solanum lycopersicum
34500
-
Leu-Leu His-tagged recombinant enzyme, 37°C Solanum lycopersicum