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Literature summary for 3.4.11.1 extracted from
- Characterization of a solvent resistant and thermostable aminopeptidase from the hyperthermophilic bacterium, Aquifex aeolicus (2000), Enzyme Microb. Technol., 27, 83-88.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Aquifex aeolicus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetics | Aquifex aeolicus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 54000 | - |
x * 54000, SDS-PAGE | Aquifex aeolicus |
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| 1-propanol | resistant to | Aquifex aeolicus |
| 2-propanol | resistant to | Aquifex aeolicus |
| Acetone | resistant to | Aquifex aeolicus |
| acetonitrile | resistant to | Aquifex aeolicus |
| dimethyl sulfoxide | resistant to | Aquifex aeolicus |
| dimethylformamide | resistant to | Aquifex aeolicus |
| dioxane | resistant to | Aquifex aeolicus |
| Ethanol | resistant to | Aquifex aeolicus |
| Methanol | resistant to | Aquifex aeolicus |
| tetrahydrofuran | resistant to | Aquifex aeolicus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aquifex aeolicus | - |
hyperthermophilic bacterium contains a highly thermostable enzyme | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant from Escherichia coli, to homogeneity | Aquifex aeolicus |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 54000, SDS-PAGE | Aquifex aeolicus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| leucine aminopeptidase | - |
Aquifex aeolicus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
about | Aquifex aeolicus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
heat stable enzyme | Aquifex aeolicus |
| 115 | - |
30 min, 27% remaining activity | Aquifex aeolicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | 8.5 | - |
Aquifex aeolicus |
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