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Literature summary for 3.4.11.1 extracted from

  • Ogata, K.; Ohno, R.; Morioka, S.; Terao, K.
    Further study on association of 5SrRNA-L5 protein complex and methionyl-tRNA to methionyl-tRNA synthetase in the macromolecular aminoacyl-tRNA synthetase complex (1996), J. Biochem., 120, 869-880.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
crystallization in presence of ZnSO4, NaCl, and 2-methyl-2,4-pentanediol, at pH 7.8 in Tris buffer, X-ray diffraction crystal structure determination and analysis at 1.6 A resolution Bos taurus

Inhibitors

Inhibitors Comment Organism Structure
amastatin natural inhibitor Bos taurus
Amino acid hydroxamates
-
Bos taurus
bestatin natural inhibitor Bos taurus
L-leucylthiol
-
Bos taurus
leucinal
-
Bos taurus
leucine phosphonic acid
-
Bos taurus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6
-
L-Leu-4-nitroanilide Zn2+-Zn2+-enzyme form Bos taurus

Metals/Ions

Metals/Ions Comment Organism Structure
bicarbonate activates Bos taurus
Mg2+ activates Bos taurus
Mn2+ activates Bos taurus
additional information the enzyme contains no disulfide bonds Bos taurus
Zn2+ zinc-metallopeptidase, two Zn2+ per protomer, the zinc bound at residue 488 can be exchanged for the less preferable Mn2+, Co2+, or Mg2+, dimetal site structure involving Asp332, Asp255, Asp273, Lys250, and Glu334 for binding of the two catalytic zinc ions, overview Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
no glycoprotein
-
Bos taurus

Purification (Commentary)

Purification (Comment) Organism
native enzyme from lens to homogeneity by ammonium sulfate fractionation, and gel filtration, the enzyme shows low solubility under low salt conditions Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
lens
-
Bos taurus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-Leu-4-nitroanilide + H2O
-
Bos taurus L-Leu + 4-nitroaniline
-
?

Subunits

Subunits Comment Organism
hexamer homohexamer, lens enzyme three-dimensional structure and protein fold, overview Bos taurus

Synonyms

Synonyms Comment Organism
LAP
-
Bos taurus
leucine aminopeptidase
-
Bos taurus
More the enzyme belongs to the peptidase family M17 Bos taurus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.7
-
L-Leu-4-nitroanilide Zn2+-Zn2+-enzyme form Bos taurus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0000013
-
amastatin
-
Bos taurus