Crystallization (Comment) | Organism |
---|---|
crystallization in presence of ZnSO4, NaCl, and 2-methyl-2,4-pentanediol, at pH 7.8 in Tris buffer, X-ray diffraction crystal structure determination and analysis at 1.6 A resolution | Bos taurus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
amastatin | natural inhibitor | Bos taurus | |
Amino acid hydroxamates | - |
Bos taurus | |
bestatin | natural inhibitor | Bos taurus | |
L-leucylthiol | - |
Bos taurus | |
leucinal | - |
Bos taurus | |
leucine phosphonic acid | - |
Bos taurus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
6 | - |
L-Leu-4-nitroanilide | Zn2+-Zn2+-enzyme form | Bos taurus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
bicarbonate | activates | Bos taurus | |
Mg2+ | activates | Bos taurus | |
Mn2+ | activates | Bos taurus | |
additional information | the enzyme contains no disulfide bonds | Bos taurus | |
Zn2+ | zinc-metallopeptidase, two Zn2+ per protomer, the zinc bound at residue 488 can be exchanged for the less preferable Mn2+, Co2+, or Mg2+, dimetal site structure involving Asp332, Asp255, Asp273, Lys250, and Glu334 for binding of the two catalytic zinc ions, overview | Bos taurus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
no glycoprotein | - |
Bos taurus |
Purification (Comment) | Organism |
---|---|
native enzyme from lens to homogeneity by ammonium sulfate fractionation, and gel filtration, the enzyme shows low solubility under low salt conditions | Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
lens | - |
Bos taurus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-Leu-4-nitroanilide + H2O | - |
Bos taurus | L-Leu + 4-nitroaniline | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | homohexamer, lens enzyme three-dimensional structure and protein fold, overview | Bos taurus |
Synonyms | Comment | Organism |
---|---|---|
LAP | - |
Bos taurus |
leucine aminopeptidase | - |
Bos taurus |
More | the enzyme belongs to the peptidase family M17 | Bos taurus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.7 | - |
L-Leu-4-nitroanilide | Zn2+-Zn2+-enzyme form | Bos taurus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0000013 | - |
amastatin | - |
Bos taurus |