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Literature summary for 3.3.2.9 extracted from

  • Draper, A.J.; Hammock, B.D.
    Inhibition of soluble and microsomal epoxide hydrolase by zinc and other metals (1999), Toxicol. Sci., 52, 26-32.
    View publication on PubMed
Search for more literature for 3.3.2.9

Inhibitors

Inhibitors Comment Organism Structure
Al3+ slight inhibition at 1 mM Homo sapiens
Al3+ slight inhibition at 1 mM Mus musculus
Ba2+ 15% inhibition at 1 mM Homo sapiens
Ba2+ 15% inhibition at 1 mM Mus musculus
Ba2+ slight inhibition at 1 mM Rattus norvegicus
Ca2+ 20% inhibition at 1 mM Homo sapiens
Ca2+ 25% inhibition at 1 mM Mus musculus
Ca2+ slight inhibition at 1 mM Rattus norvegicus
Cd2+ 20% inhibition at 1 mM Homo sapiens
Cd2+ 70% inhibition at 1 mM Mus musculus
Cd2+ 20% inhibition at 1 mM Rattus norvegicus
Co2+ 20% inhibition at 1 mM Homo sapiens
Co2+ 25% inhibition at 1 mM Rattus norvegicus
Cu2+ slight inhibition at 1 mM Homo sapiens
Cu2+ 25% inhibition at 1 mM Mus musculus
Cu2+ slight inhibition at 1 mM Rattus norvegicus
Hg2+ 90% inhibition at 1 mM Homo sapiens
Hg2+ over 90% inhibition at 1 mM Mus musculus
Hg2+ 70% inhibition at 1 mM Rattus norvegicus
Mg2+ 30% inhibition at 1 mM Homo sapiens
Mg2+ 10% inhibition at 1 mM Mus musculus
Mg2+ 10% inhibition at 1 mM Rattus norvegicus
Mn2+ 10% inhibition at 1 mM Homo sapiens
Mn2+ 20% inhibition at 1 mM Mus musculus
Mn2+ 10% inhibition at 1 mM Rattus norvegicus
additional information metal chelators like 1,10-phenanthroline, 1,7-phenanthroline, EDTA, EGTA, and dipicolinic acid preserve enzyme activity in presence of metal ions Homo sapiens
additional information no inhibition by Co2+ at 1 mM, metal chelators like 1,10-phenanthroline, 1,7-phenanthroline, EDTA, EGTA, and dipicolinic acid preserve enzyme activity in presence of metal ions Mus musculus
additional information metal chelators like 1,10-phenanthroline, 1,7-phenanthroline, EDTA, EGTA, and dipicolinic acid preserve enzyme activity in presence of metal ions Rattus norvegicus
Ni2+ 30% inhibition at 1 mM Homo sapiens
Ni2+ 30% inhibition at 1 mM Mus musculus
Ni2+ 30% inhibition at 1 mM Rattus norvegicus
Pb2+ 10% inhibition at 1 mM Homo sapiens
Pb2+ 30% inhibition at 1 mM Mus musculus
Pb2+ 10% inhibition at 1 mM Rattus norvegicus
Zn2+ 80% inhibition at 1 mM Homo sapiens
Zn2+ over 90% inhibition at 1 mM Mus musculus
Zn2+ 80% inhibition at 1 mM Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0024
-
 cis-stilbene oxide pH 7.4, 37°C Rattus norvegicus
0.004
-
 cis-stilbene oxide pH 7.4, 37°C Homo sapiens
0.015
-
 cis-stilbene oxide pH 7.4, 37°C Mus musculus

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
 Mus musculus
-
-
microsome
-
 Homo sapiens
-
-
microsome
-
 Rattus norvegicus
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Al3+ slight inhibition at 1 mM Mus musculus
Al3+ slight inhibition at 1 mM Homo sapiens
Ba2+ 15% inhibition at 1 mM Mus musculus
Ba2+ 15% inhibition at 1 mM Homo sapiens
Ba2+ slight inhibition at 1 mM Rattus norvegicus
Ca2+ 20% inhibition at 1 mM Homo sapiens
Ca2+ 25% inhibition at 1 mM Mus musculus
Ca2+ slight inhibition at 1 mM Rattus norvegicus
Cd2+ 20% inhibition at 1 mM Homo sapiens
Cd2+ 20% inhibition at 1 mM Rattus norvegicus
Cd2+ 70% inhibition at 1 mM Mus musculus
Co2+ 20% inhibition at 1 mM Homo sapiens
Co2+ 25% inhibition at 1 mM Rattus norvegicus
Cu2+ 25% inhibition at 1 mM Mus musculus
Cu2+ slight inhibition at 1 mM Homo sapiens
Cu2+ slight inhibition at 1 mM Rattus norvegicus
Fe2+ 10% activation at 1 mM Rattus norvegicus
Fe2+ 15%% activation at 1 mM Mus musculus
Fe2+ 40% activation at 1 mM Homo sapiens
Fe3+ 20% activation at 1 mM Rattus norvegicus
Fe3+ 30%% activation at 1 mM Mus musculus
Fe3+ 50% activation at 1 mM Homo sapiens
Hg2+ 70% inhibition at 1 mM Rattus norvegicus
Hg2+ 90% inhibition at 1 mM Homo sapiens
Hg2+ over 90% inhibition at 1 mM Mus musculus
Mg2+ 10% inhibition at 1 mM Mus musculus
Mg2+ 10% inhibition at 1 mM Rattus norvegicus
Mg2+ 30% inhibition at 1 mM Homo sapiens
Mn2+ 10% inhibition at 1 mM Homo sapiens
Mn2+ 10% inhibition at 1 mM Rattus norvegicus
Mn2+ 20% inhibition at 1 mM Mus musculus
additional information no effect by 1 mM Al3+ Rattus norvegicus
Ni2+ 30% inhibition at 1 mM Mus musculus
Ni2+ 30% inhibition at 1 mM Homo sapiens
Ni2+ 30% inhibition at 1 mM Rattus norvegicus
Pb2+ 10% inhibition at 1 mM Homo sapiens
Pb2+ 10% inhibition at 1 mM Rattus norvegicus
Pb2+ 30% inhibition at 1 mM Mus musculus
Zn2+ 80% inhibition at 1 mM Homo sapiens
Zn2+ 80% inhibition at 1 mM Rattus norvegicus
Zn2+ over 90% inhibition at 1 mM Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
cis-stilbene oxide + H2O Mus musculus
-
 (+)-(1R,2R)-1,2-diphenylethane-1,2-diol
-
 ?
cis-stilbene oxide + H2O Homo sapiens
-
 (+)-(1R,2R)-1,2-diphenylethane-1,2-diol
-
 ?
cis-stilbene oxide + H2O Rattus norvegicus
-
 (+)-(1R,2R)-1,2-diphenylethane-1,2-diol
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Mus musculus
-
 male Swiss Webster mice
-
Rattus norvegicus
-
 male F344 rats
-

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Mus musculus
-
liver
-
 Homo sapiens
-
liver
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cis-stilbene oxide + H2O
-
 Mus musculus (+)-(1R,2R)-1,2-diphenylethane-1,2-diol
-
 ?
cis-stilbene oxide + H2O
-
 Homo sapiens (+)-(1R,2R)-1,2-diphenylethane-1,2-diol
-
 ?
cis-stilbene oxide + H2O
-
 Rattus norvegicus (+)-(1R,2R)-1,2-diphenylethane-1,2-diol
-
 ?

Synonyms

Synonyms Comment Organism
mEH
-
 Mus musculus
mEH
-
 Homo sapiens
mEH
-
 Rattus norvegicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Mus musculus
37
-
 assay at Homo sapiens
37
-
 assay at Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Mus musculus
7.4
-
 assay at Homo sapiens
7.4
-
 assay at Rattus norvegicus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0076
-
 Zn2+ pH 7.4, 37°C, mixed inhibition type Mus musculus
0.012
-
 Zn2+ pH 7.4, 37°C, competitive inhibition type Rattus norvegicus
0.061
-
 Zn2+ pH 7.4, 37°C, competitive inhibition type Homo sapiens