Crystallization (Comment) | Organism |
---|---|
selenomethionine-substituted enzyme | Rhodococcus erythropolis |
Protein Variants | Comment | Organism |
---|---|---|
D101A | catalytically inactive, D101 is the acid catalyst that protonates the epoxide oxygen | Rhodococcus erythropolis |
D101N | catalytically inactive, D101 is the acid catalyst that protonates the epoxide oxygen | Rhodococcus erythropolis |
N55A | little residual activity | Rhodococcus erythropolis |
N55D | catalytically inactive | Rhodococcus erythropolis |
N55D/D132N | catalytically inactive, folding correct | Rhodococcus erythropolis |
R99A | catalytically inactive | Rhodococcus erythropolis |
R99H | catalytically inactive | Rhodococcus erythropolis |
R99K | catalytically inactive | Rhodococcus erythropolis |
R99Q | catalytically inactive | Rhodococcus erythropolis |
Y53F | partially active | Rhodococcus erythropolis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
hexanamide | competitive | Rhodococcus erythropolis | |
Hexylamine | competitive | Rhodococcus erythropolis | |
valpromide | i.e. dipropylacetamide, competitive | Rhodococcus erythropolis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.4 | - |
styrene-7,8-oxide | pH 7.4, mutant N55A | Rhodococcus erythropolis | |
1.4 | - |
styrene-7,8-oxide | pH 7.4, wild-type | Rhodococcus erythropolis | |
3.7 | - |
styrene-7,8-oxide | pH 7.4, mutant Y53F | Rhodococcus erythropolis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodococcus erythropolis | Q9ZAG3 | - |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
1,2-epoxymenth-8-ene + H2O = menth-8-ene-1,2-diol | one-step mechanism | Rhodococcus erythropolis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
styrene-7,8-oxide + H2O | - |
Rhodococcus erythropolis | styrene-7,8-diol | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | crystallization data | Rhodococcus erythropolis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.002 | - |
styrene-7,8-oxide | pH 7.4, mutant N55A | Rhodococcus erythropolis | |
0.176 | - |
styrene-7,8-oxide | pH 7.4, mutant Y53F | Rhodococcus erythropolis | |
0.47 | - |
styrene-7,8-oxide | pH 7.4, wild-type | Rhodococcus erythropolis |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.035 | - |
Hexylamine | pH 7.4, 1% ethanol in final assay mixture | Rhodococcus erythropolis | |
0.1 | - |
valpromide | pH 7.4, 1% ethanol in final assay mixture | Rhodococcus erythropolis | |
2 | - |
hexanamide | pH 7.4, 1% ethanol in final assay mixture | Rhodococcus erythropolis |