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Literature summary for 3.3.2.6 extracted from
- Saccharomyces cerevisiae leukotriene A4 hydrolase: formation of leukotriene B4 and identification of catalytic residues (2001), Biochemistry, 40, 12695-12703.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| leukotriene A4 | stimulates strong aminopeptidase activity via lipid binding site | Saccharomyces cerevisiae |  |
Application
| Application | Comment | Organism |
|---|---|---|
| medicine | the enzyme catalyses the hydrolysis of leukotriene A4 into the proinflammatory substance leukotriene B4 | Homo sapiens |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli and in Sf9 cells, using the baculovirus system | Saccharomyces cerevisiae |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| E341Q | 101% hydrolase activity, 0.07% peptidase activity, in comparison to 100% hydrolase- and peptidase activity of wild-type enzyme | Saccharomyces cerevisiae |
| E363Q | 2.1% hydrolase activity, 0.05% peptidase activity, in comparison to 100% hydrolase- and peptidase activity of wild-type enzyme | Saccharomyces cerevisiae |
| F424Y | 39.5% hydrolase activity, 147% peptidase activity, in comparison to 100% hydrolase- and peptidase activity of wild-type enzyme | Saccharomyces cerevisiae |
| H340Q | 1.5% hydrolase activity, 0.7% peptidase activity, in comparison to 100% hydrolase- and peptidase activity of wild-type enzyme | Saccharomyces cerevisiae |
| H344Q | 1% hydrolase activity, 0.2% peptidase activity, in comparison to 100% hydrolase- and peptidase activity of wild-type enzyme | Saccharomyces cerevisiae |
| Y429F | 16.4% hydrolase activity, 0.6% peptidase activity, in comparison to 100% hydrolase- and peptidase activity of wild-type enzyme | Saccharomyces cerevisiae |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| leukotriene A4 | inhibits epoxid hydrolase activity and stimulates peptidase activity | Saccharomyces cerevisiae | |
| leukotriene A4 methyl ester | - |
Homo sapiens | |
| leukotriene A4 methyl ester | no influence | Saccharomyces cerevisiae | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Homo sapiens | |
| additional information | - |
additional information | - |
Saccharomyces cerevisiae | |
| 0.06 | - |
leukotriene A4 | pH 7.5, 22°C, 5S,6S-dihydroxy-7,9-trans-11,14-cis-eicosatetraenoic acid production | Saccharomyces cerevisiae | |
| 2.1 | - |
L-leucine-4-nitroanilide | pH 7.5, 22°C, enzyme stimulated by leukotriene A4 | Saccharomyces cerevisiae | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Zinc | three putative zinc-binding ligands: His340, His344 and Glu363, zinc atom is catalytic and also involved in the maintenance of the structural integrity of the enzyme | Saccharomyces cerevisiae | |
| Zinc | zinc protein | Homo sapiens | |
| Zinc | zinc protein | Saccharomyces cerevisiae | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| leukotriene A4 + H2O | Homo sapiens | biosynthesis of leukotriene B4 | leukotriene B4 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
| Saccharomyces cerevisiae | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Saccharomyces cerevisiae |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| leukotriene A4 + H2O = leukotriene B4 | bifunctional enzyme acting as an epoxide hydrolase and also as an aminopeptidase | Homo sapiens | |
| leukotriene A4 + H2O = leukotriene B4 | bifunctional enzyme acting as an epoxide hydrolase and also as an aminopeptidase | Saccharomyces cerevisiae |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Homo sapiens |
| additional information | - |
- |
Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-leucine-4-nitroanilide + H2O | - |
Saccharomyces cerevisiae | L-leucine + 4-nitroaniline | - |
? | |
| leukotriene A4 + H2O | - |
Homo sapiens | leukotriene B4 | - |
? | |
| leukotriene A4 + H2O | biosynthesis of leukotriene B4 | Homo sapiens | leukotriene B4 | - |
? | |
| leukotriene A4 + H2O | - |
Saccharomyces cerevisiae | (5S,6S)-dihydroxy-7,9-trans-11,14-cis-eicosatetraenoic acid + leukotriene B4 + (5S,12R)-dihydroxy-6,10-trans-8,14,cis-eicosatetraenoic acid | when large amounts of enzyme are incubated with leukotriene A4: 72% (5S,6S)-dihydroxy-7,9-trans-11,14-cis-eicosatetraenoic acid, 18% leukotriene B4 and 10% (5S,12R)-dihydroxy-6,10-trans-8,14,cis-eicosatetraenoic acid | ? | |
| additional information | - |
Homo sapiens | ? | - |
? | |
| additional information | - |
Saccharomyces cerevisiae | ? | - |
? | |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Saccharomyces cerevisiae |
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