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Literature summary for 3.3.2.6 extracted from

  • Stromberg-Kull, F.; Haeggstrom, J.Z.
    Purification and characterization of leukotriene A4 hydrolase from Xenopus laevis oocytes (1998), FEBS Lett., 433, 219-222.
    View publication on PubMed

Application

Application Comment Organism
medicine the enzyme catalyses the hydrolysis of leukotriene A4 into the proinflammatory substance leukotriene B4 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0027
-
leukotriene A4 pH 8.0, 22°C Homo sapiens
0.02
-
leukotriene A4 pH 8.0, 22°C Xenopus laevis

Metals/Ions

Metals/Ions Comment Organism Structure
Zinc
-
Homo sapiens
Zinc 0.8 mol zinc per mol enzyme Xenopus laevis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
69000
-
x * 69000, SDS-PAGE Xenopus laevis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
leukotriene A4 + H2O Homo sapiens biosynthesis of leukotriene B4 leukotriene B4
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Xenopus laevis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Xenopus laevis

Reaction

Reaction Comment Organism Reaction ID
leukotriene A4 + H2O = leukotriene B4 bifunctional enzyme acting as an epoxide hydrolase and also as an aminopeptidase Homo sapiens
leukotriene A4 + H2O = leukotriene B4 bifunctional enzyme acting as an epoxide hydrolase and also as an aminopeptidase Xenopus laevis

Source Tissue

Source Tissue Comment Organism Textmining
oocyte
-
Xenopus laevis
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.7
-
-
Xenopus laevis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
leukotriene A4 + H2O
-
Homo sapiens leukotriene B4
-
?
leukotriene A4 + H2O
-
Xenopus laevis leukotriene B4
-
?
leukotriene A4 + H2O biosynthesis of leukotriene B4 Homo sapiens leukotriene B4
-
?
leukotriene A4 + H2O
-
Xenopus laevis leukotriene B4 + (5S,12R)-dihydroxy-6,10-trans-8,14,cis-eicosatetraenoic acid 90% leukotriene B4 and 10% (5S,12R)-dihydroxy-6,10-trans-8,14,cis-eicosatetraenoic acid ?
additional information
-
Homo sapiens ?
-
?
additional information
-
Xenopus laevis ?
-
?

Subunits

Subunits Comment Organism
?
-
Homo sapiens
? x * 69000, SDS-PAGE Xenopus laevis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.5
-
leukotriene A4 pH 8.0, 22°C Homo sapiens
14
-
leukotriene A4 pH 8.0, 22°C Xenopus laevis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
Xenopus laevis

pI Value

Organism Comment pI Value Maximum pI Value
Xenopus laevis isoelectric focusing and chromatofocusing
-
6.1