Application | Comment | Organism |
---|---|---|
medicine | the enzyme catalyses the hydrolysis of leukotriene A4 into the proinflammatory substance leukotriene B4 | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0027 | - |
leukotriene A4 | pH 8.0, 22°C | Homo sapiens | |
0.02 | - |
leukotriene A4 | pH 8.0, 22°C | Xenopus laevis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zinc | - |
Homo sapiens | |
Zinc | 0.8 mol zinc per mol enzyme | Xenopus laevis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
69000 | - |
x * 69000, SDS-PAGE | Xenopus laevis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
leukotriene A4 + H2O | Homo sapiens | biosynthesis of leukotriene B4 | leukotriene B4 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Xenopus laevis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Xenopus laevis |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
leukotriene A4 + H2O = leukotriene B4 | bifunctional enzyme acting as an epoxide hydrolase and also as an aminopeptidase | Homo sapiens | |
leukotriene A4 + H2O = leukotriene B4 | bifunctional enzyme acting as an epoxide hydrolase and also as an aminopeptidase | Xenopus laevis |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
oocyte | - |
Xenopus laevis | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
6.7 | - |
- |
Xenopus laevis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
leukotriene A4 + H2O | - |
Homo sapiens | leukotriene B4 | - |
? | |
leukotriene A4 + H2O | - |
Xenopus laevis | leukotriene B4 | - |
? | |
leukotriene A4 + H2O | biosynthesis of leukotriene B4 | Homo sapiens | leukotriene B4 | - |
? | |
leukotriene A4 + H2O | - |
Xenopus laevis | leukotriene B4 + (5S,12R)-dihydroxy-6,10-trans-8,14,cis-eicosatetraenoic acid | 90% leukotriene B4 and 10% (5S,12R)-dihydroxy-6,10-trans-8,14,cis-eicosatetraenoic acid | ? | |
additional information | - |
Homo sapiens | ? | - |
? | |
additional information | - |
Xenopus laevis | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | - |
Homo sapiens |
? | x * 69000, SDS-PAGE | Xenopus laevis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.5 | - |
leukotriene A4 | pH 8.0, 22°C | Homo sapiens | |
14 | - |
leukotriene A4 | pH 8.0, 22°C | Xenopus laevis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Xenopus laevis |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Xenopus laevis | isoelectric focusing and chromatofocusing | - |
6.1 |