Application | Comment | Organism |
---|---|---|
medicine | the enzyme catalyses the hydrolysis of leukotriene A4 into the proinflammatory substance leukotriene B4 | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
E297A | 30% hydrolase activity, 25% peptidase activity, in comparison to 100% hydrolase- and peptidase activity of wild-type enzyme | Homo sapiens |
E297D | 17% hydrolase activity, 38% peptidase activity, in comparison to 100% hydrolase- and peptidase activity of wild-type enzyme | Homo sapiens |
E297K | 13% hydrolase activity, 16% peptidase activity, in comparison to 100% hydrolase- and peptidase activity of wild-type enzyme | Homo sapiens |
E297Q | 104% hydrolase activity, 14% peptidase activity, in comparison to 100% hydrolase- and peptidase activity of wild-type enzyme | Homo sapiens |
E319K | 0% hydrolase activity, 12% peptidase activity, in comparison to 100% hydrolase- and peptidase activity of wild-type enzyme | Homo sapiens |
Y384F | 0% hydrolase activity, 13% peptidase activity, in comparison to 100% hydrolase- and peptidase activity of wild-type enzyme | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zinc | zinc protein | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
leukotriene A4 + H2O | Homo sapiens | biosynthesis of leukotriene B4 | leukotriene B4 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
leukotriene A4 + H2O | - |
Homo sapiens | leukotriene B4 | - |
? | |
leukotriene A4 + H2O | biosynthesis of leukotriene B4 | Homo sapiens | leukotriene B4 | - |
? | |
additional information | either glutamic or glutamine moiety at amino acid 297 is required for full LTA4 hydrolase activity, free carboxylic acid of glutamic acid is essential for aminopeptidase | Homo sapiens | ? | - |
? |