Application | Comment | Organism |
---|---|---|
medicine | the enzyme catalyses the hydrolysis of leukotriene A4 into the proinflammatory substance leukotriene B4 | Mus musculus |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli as a fusion protein with ten additional amino acids at the amino terminus | Mus musculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Mus musculus | |
0.005 | - |
leukotriene A4 | pH 8.0, 0°C | Mus musculus | |
0.68 | - |
L-alanine-4-nitroanilide | pH 8.0, 37°C | Mus musculus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | apoenzyme is inactive and can be reactivated by addition of stoichiometric amounts of zinc and cobalt | Mus musculus | |
Zinc | apoenzyme is inactive and can be reactivated by addition of stoichiometric amounts of zinc and cobalt | Mus musculus | |
Zinc | zinc content: 1 mol of zinc per mol of enzyme | Mus musculus | |
Zinc | zinc protein | Mus musculus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
69000 | - |
x * 69000, SDS-PAGE | Mus musculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme, expression in Escherichia coli as a fusion protein with ten additional amino acids at the amino terminus | Mus musculus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
leukotriene A4 + H2O = leukotriene B4 | bifunctional enzyme acting as an epoxide hydrolase and also as an aminopeptidase | Mus musculus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.185 | - |
- |
Mus musculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-alanine-4-nitroanilide + H2O | - |
Mus musculus | L-alanine + 4-nitroaniline | - |
? | |
leukotriene A4 + H2O | - |
Mus musculus | leukotriene B4 | - |
? | |
additional information | - |
Mus musculus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 69000, SDS-PAGE | Mus musculus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Mus musculus | chromatofocusing | - |
5.5 |