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Literature summary for 3.3.2.11 extracted from
- Distinct rat hepatic microsomal epoxide hydrolases catalyze the hydration of cholesterol 5,6alpha-oxide and certain xenobiotic alkene and arene oxides (1983), Arch. Biochem. Biophys., 220, 485-494.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| acetonitrile | maximal enzyme activity at 6.25% v/v, 19% of maximal activity at 1.25% v/v, 50% of maximal activity at 12.5% v/v, no activity at 18.8% v/v | Rattus norvegicus |  |
| additional information | no effect by 5,6alpha-epoxy-5alpha-cholestan-3beta-ol in vivo, and by EDTA and 2,2-dipyridyl, preferred buffer is 300 mM potassium phosphate at pH 7.4 and 37°C, low activity in Tris and HEPES buffers | Rattus norvegicus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 3-methylcholanthracene | - |
Rattus norvegicus | |
| acetone | reduces the activity by 85% at 1.25% v/v, by 50% at 3.75% v/v, and by 56% at 6.25% v/v | Rattus norvegicus | |
| Aroclor 1254 | - |
Rattus norvegicus | |
| benz[a]anthracene 5,6-oxide | non-competitive inhibition | Rattus norvegicus | |
| cholestyramine | - |
Rattus norvegicus | |
| Clofibrate | - |
Rattus norvegicus | |
| Dimethylsulfoxide | reduces the activity by 42% at 6.25% v/v | Rattus norvegicus | |
| gamma-chlordane | - |
Rattus norvegicus | |
| isosafrole | slight inhibition | Rattus norvegicus | |
| methanol | reduces the activity by 86% at 3.75% v/v | Rattus norvegicus | |
| additional information | no effect by 5,6alpha-epoxy-5alpha-cholestan-3beta-ol in vivo, and by EDTA and 2,2-dipyridyl, preferred buffer is 300 mM potassium phosphate at pH 7.4 and 37°C, low activity in Tris and HEPES buffers | Rattus norvegicus | |
| octene 1,2-oxide | non-competitive inhibition | Rattus norvegicus | |
| Phenobarbital | - |
Rattus norvegicus | |
| pregnenolone 16alpha-carbonitrile | - |
Rattus norvegicus | |
| tetrahydrofuran | reduces the activity by 86% at 6.25% v/v | Rattus norvegicus | |
| trans-stilbene oxide | - |
Rattus norvegicus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| microsome | - |
Rattus norvegicus | - |
- |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5,6alpha-epoxy-5alpha-cholestan-3beta-ol + H2O | Rattus norvegicus | regulation, the enzyme is highly substrate specific | cholestane-3beta,5alpha,6beta-triol | - |
? | |
| additional information | Rattus norvegicus | the enzyme is distinct from the microsomal epoxide hydrolase EC 3.3.2.9, which shows a wider xenobiotic substrate specificity | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
male Long Evans rats | - |
Oxidation Stability
| Oxidation Stability | Organism |
|---|---|
| acetone reduces the activity by 85% at 1.25% v/v, by 50% at 3.75% v/v, and by 56% at 6.25% v/v | Rattus norvegicus |
| acetonitrile activates the enzyme at moderate concentration, maximal enzyme activity at 6.25% v/v, 19% of maximal activity at 1.25% v/v, 50% of maximal activity at 12.5% v/v, no activity at 18.8% v/v | Rattus norvegicus |
| dimethylsulfoxide reduces the activity by 42% at 6.25% v/v | Rattus norvegicus |
| methanol reduces the activity by 86% at 3.75% v/v | Rattus norvegicus |
| tetrahydrofuran reduces the activity by 86% at 6.25% v/v | Rattus norvegicus |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partially from liver by micosome preparation and immunoprecipitation, separation from the xenobiotic epoxide hydrolase, EC 3.3.2.9 | Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
evaluation of substrate solubilization solvents for enzyme assay, preferred buffer is 300 mM potassium phosphate at pH 7.4 and 37°C | Rattus norvegicus |
| 0.00123 | - |
liver microsomes | Rattus norvegicus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -90°C, liver microsomes, enzyme is stable during repeated freezing and thawing | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5,6alpha-epoxy-5alpha-cholestan-3beta-ol + H2O | regulation, the enzyme is highly substrate specific | Rattus norvegicus | cholestane-3beta,5alpha,6beta-triol | - |
? | |
| 5,6alpha-epoxy-5alpha-cholestan-3beta-ol + H2O | the enzyme is highly substrate-specific | Rattus norvegicus | cholestane-3beta,5alpha,6beta-triol | i.e. 5,6-glycol | ? | |
| additional information | the enzyme is distinct from the microsomal epoxide hydrolase EC 3.3.2.9, which shows a wider xenobiotic substrate specificity | Rattus norvegicus | ? | - |
? | |
| additional information | no activity with xenobiotic alkene oxides and arene oxides, e.g. octene 1,2-oxide or benz[a]anthracene 5,6-oxide | Rattus norvegicus | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Rattus norvegicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
- |
Rattus norvegicus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.8 | 9 | 80-90% of maximal activity at pH 6.8 and pH 7.7, 33% at pH 9.0 | Rattus norvegicus |
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