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Literature summary for 3.3.2.10 extracted from

  • Abis, G.; Charles, R.L.; Eaton, P.; Conte, M.R.
    Expression, purification, and characterisation of human soluble epoxide hydrolase (hsEH) and of its functional C-terminal domain (2019), Protein Expr. Purif., 153, 105-113 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.3.2.10

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression of soluble full-length enzyme in HEK293-F cells via baculovirus transfection system, recombinant expression of the C-terminal domain in Escherichia coli strain Ros2(DE3) and of N-terminally His-tagged full-length enzyme in Escherichia coli strain DH5alpha C2987, method optimization Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
(1R,3S)-N-(4-cyano-2-(trifluoromethyl)benzyl)-3-((4-methyl-6-(methylamino)-1,3,5-triazin-2-yl)amino)cyclohexanecarboxamide GSK2256294 Homo sapiens
1-(1-acetylpiperidin-4-yl)-3-adamantan-1-yl-urea AR9281 Homo sapiens
12-[[(tricyclo[3.3.1.13,7]dec-1-ylamino)carbonyl]amino]-dodecanoic acid AUDA Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens P34913
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme and C-terminal domain from Escherichia coli by nickel or benzylthio affinity chromatography and gel filtration Homo sapiens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
36.29
-
 purified recombinant full-length enzyme, pH 7.4, 30°C Homo sapiens
67.64
-
 purified recombinant C-terminal domain, pH 7.4, 30°C Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-phenyl-cyano(6-methoxy-2-naphthalenyl)methyl ester-2-oxiraneacetic acid + H2O
-
 Homo sapiens 6-methoxy-2-naphtaldehyde + ?
-
 ?

Subunits

Subunits Comment Organism
? x * 39498, sequence calculation Homo sapiens

Synonyms

Synonyms Comment Organism
hsEH
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Homo sapiens

General Information

General Information Comment Organism
physiological function the human soluble Epoxide Hydrolase (hsEH) is an enzyme involved in the hydrolysis of endogenous anti-inflammatory and cardio-protective signalling mediators known as epoxyeicosatrienoic acids (EETs). EETs' conversion into the corresponding diols by hsEH generates non-bioactive molecules, thereby the enzyme inhibition is be expected to enhance the EETs bioavailability, and their beneficial properties Homo sapiens