Cloned (Comment) | Organism |
---|---|
gene EPHX2, quantitative real-time PCR expression analysis | Mus musculus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of sEH-/- knockout mice, phenotype compared to wild-type, overview | Mus musculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
trans-4-[4-(3-adamantan-1-ylureido)cyclohexyloxy]-benzoic acid | i.e. t-AUCB | Mus musculus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Mus musculus | 5829 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | P34914 | - |
- |
Mus musculus C57BL/6 | P34914 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
hepatocyte | - |
Mus musculus | - |
kidney | - |
Mus musculus | - |
liver | - |
Mus musculus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(9Z)-12,13-epoxyoctadecenoic acid + H2O | - |
Mus musculus | (9Z)-12,13-dihydroxyoctadecenoic acid | - |
? | |
(9Z)-12,13-epoxyoctadecenoic acid + H2O | - |
Mus musculus C57BL/6 | (9Z)-12,13-dihydroxyoctadecenoic acid | - |
? | |
additional information | the sEH is a dual function enzyme that generates dihydroxy-fatty acids via its C-terminal hydrolase domain, while the N-terminal phosphatase domain has been proposed to have lipid phosphatase activity, bifunctional epoxide hydrolase 2 includes cytosolic epoxide hydrolase 2, sEH, EC 3.3.2.10, and lipid-phosphate phosphatase, EC 3.1.3.76 | Mus musculus | ? | - |
? | |
additional information | the sEH is a dual function enzyme that generates dihydroxy-fatty acids via its C-terminal hydrolase domain, while the N-terminal phosphatase domain has been proposed to have lipid phosphatase activity, bifunctional epoxide hydrolase 2 includes cytosolic epoxide hydrolase 2, sEH, EC 3.3.2.10, and lipid-phosphate phosphatase, EC 3.1.3.76 | Mus musculus C57BL/6 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
SEH | - |
Mus musculus |
General Information | Comment | Organism |
---|---|---|
malfunction | deletion of sEH decreases expression of HMG-CoA reductase, fatty acid synthase, and low density lipoprotein receptor. Sterol regulatory element binding proteins (SREBPs) regulate the expression of all three enzymes and SREBP activation is attenuated in the absence of sEH. The effect is attributed to the AMPK-activated protein kinase (AMPK) which is activated in the absence of sEH. Livers from wild-type versus sEH-/- littermates contain significantly higher levels of the sEH substrate 12,13-epoxyoctadecenoic acid, which elicits dAMPK activation, while the corresponding sEH product is inactive. Thus, AMPK activation and subsequent inhibition of SREBP can account for the altered expression of lipid metabolizing enzymes in sEH-/- mice | Mus musculus |