Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of N-truncated murine EH3 and N-truncated+7aa-insert murine EH3 mutants | Mus musculus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Homo sapiens | 5829 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
9R,10R-trans-epoxy-13R-hydroxy-11E-octadecenoic acid + H2O | Homo sapiens | - |
(9R,10S,13R)-trihydroxy-11E-octadecenoic acid | - |
? | |
9R,10R-trans-epoxy-13R-hydroxy-11E-octadecenoic acid + H2O | Mus musculus | - |
(9R,10S,13R)-trihydroxy-11E-octadecenoic acid | - |
? | |
9R,10S-cis-epoxy-13R-hydroxy-11E-octadecenoic acid | Homo sapiens | - |
(9R,10R,13R)-trihydroxy-11E-octadecenoic acid | - |
? | |
cis-14,15-epoxyeicosatrienoic acid + H2O | Homo sapiens | - |
cis-14,15-dihydroxyeicosatrienoic acid | - |
? | |
cis-14,15-epoxyeicosatrienoic acid + H2O | Mus musculus | - |
cis-14,15-dihydroxyeicosatrienoic acid | - |
? | |
additional information | Homo sapiens | the enzyme converts epoxyalcohols into linoleate triols | ? | - |
? | |
additional information | Mus musculus | the enzyme converts epoxyalcohols into linoleate triols | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P34913 | - |
- |
Homo sapiens | Q9H6B9 | - |
- |
Mus musculus | Q3V1F8 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
epidermis | - |
Homo sapiens | - |
epidermis | - |
Mus musculus | - |
skin | EH3 is highly expressed in the skin, the highest expression of EH3 mRNA is in the outer epidermis where the water permeability barrier is formed | Homo sapiens | - |
skin | EH3 is highly expressed in the skin, the highest expression of EH3 mRNA is in the outer epidermis where the water permeability barrier is formed | Mus musculus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
12,13-cis-epoxy-octadeca-9E-enoic acid + H2O | - |
Homo sapiens | ? | - |
? | |
12,13-trans-epoxy-octadeca-9E-enoic acid + H2O | - |
Homo sapiens | ? | - |
? | |
9R,10R-trans-epoxy-13R-hydroxy-11E-octadecenoic acid + H2O | - |
Homo sapiens | (9R,10S,13R)-trihydroxy-11E-octadecenoic acid | - |
? | |
9R,10R-trans-epoxy-13R-hydroxy-11E-octadecenoic acid + H2O | - |
Mus musculus | (9R,10S,13R)-trihydroxy-11E-octadecenoic acid | - |
? | |
9R,10S-cis-epoxy-13R-hydroxy-11E-octadecenoic acid | - |
Homo sapiens | (9R,10R,13R)-trihydroxy-11E-octadecenoic acid | - |
? | |
9R,10S-cis-epoxy-13R-hydroxy-11E-octadecenoic acid + H2O | - |
Homo sapiens | (9R,10R,13R)-trihydroxy-11E-octadecenoic acid | - |
? | |
cis-14,15-epoxyeicosatrienoic acid + H2O | - |
Homo sapiens | cis-14,15-dihydroxyeicosatrienoic acid | - |
? | |
cis-14,15-epoxyeicosatrienoic acid + H2O | - |
Mus musculus | cis-14,15-dihydroxyeicosatrienoic acid | - |
? | |
additional information | the enzyme converts epoxyalcohols into linoleate triols | Homo sapiens | ? | - |
? | |
additional information | the enzyme converts epoxyalcohols into linoleate triols | Mus musculus | ? | - |
? | |
additional information | human soluble EH (sEH or EPHX2) and human or murine epoxide hydrolase-3 (EH3 or EPHX3) hydrolyze cis or trans allylic epoxides to single diastereomers, identical to the major isomers detected in epidermis. Microsomal EH (mEH or EPHX1, EC 3.3.2.9) is inactive with these substrates. 12,13-trans-Epoxy-octadeca-9E-enoic acid is completely hydrolyzed by human sEH, human EH3, and N-truncated murine EH3 and mostly hydrolyzed using the N-truncated+7aa-insert murine EH3. 12,13-cis-Epoxy-octadeca-9E-enoic acid is hardly hydrolyzed by human EH3, N-truncated murine EH3, and the N-truncated+7aa-insert murine EH3, and 30% hydrolysis is observed using human sEH. At low substrate concentrations, EPHX2 hydrolyzes 14,15-epoxyeicosatrienoic acid (EET) at twice the rate of the epidermal epoxyalcohol, 9R,10R-trans-epoxy-11E-13R-hydroxy-octadecenoic acid, whereas human or murine EPHX3 hydrolyze the allylic epoxyalcohol at 31fold and 39fold higher rates, respectively | Homo sapiens | ? | - |
? | |
additional information | human soluble EH (sEH or EPHX2) and human or murine epoxide hydrolase-3 (EH3 or EPHX3) hydrolyze cis or trans allylic epoxides to single diastereomers, identical to the major isomers detected in epidermis. Microsomal EH (mEH or EPHX1, EC 3.3.2.9) is inactive with these substrates. 12,13-trans-Epoxy-octadeca-9E-enoic acid is completely hydrolyzed by human sEH, human EH3, and N-truncated murine EH3 and mostly hydrolyzed using the N-truncated+7aa-insert murine EH3. 12,13-cis-Epoxy-octadeca-9E-enoic acid is hardly hydrolyzed by human EH3, N-truncated murine EH3, and the N-truncated+7aa-insert murine EH3, and 30% hydrolysis is observed using human sEH. At low substrate concentrations, EPHX2 hydrolyzes 14,15-epoxyeicosatrienoic acid (EET) at twice the rate of the epidermal epoxyalcohol, 9R,10R-trans-epoxy-11E-13R-hydroxy-octadecenoic acid, whereas human or murine EPHX3 hydrolyze the allylic epoxyalcohol at 31fold and 39fold higher rates, respectively | Mus musculus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
EH3 | - |
Homo sapiens |
EH3 | - |
Mus musculus |
EPHX2 | - |
Homo sapiens |
EPHX3 | - |
Homo sapiens |
EPHX3 | - |
Mus musculus |
epoxide hydrolase-3 | - |
Homo sapiens |
epoxide hydrolase-3 | - |
Mus musculus |
SEH | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
37 | - |
assay at | Mus musculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
with allylic epoxyalcohols as substrates | Homo sapiens |
5 | - |
with allylic epoxyalcohols as substrates | Mus musculus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 6 | maximal activity at pH 5.0, 70% of maximal activity at pH 6.0, no activity at pH 7.0-8.0, with allylic epoxyalcohols as substrates | Homo sapiens |
5 | 6 | maximal activity at pH 5.0, 70% of maximal activity at pH 6.0, no activity at pH 7.0-8.0, with allylic epoxyalcohols as substrates | Mus musculus |
General Information | Comment | Organism |
---|---|---|
additional information | evaluation of different epoxide hydrolase (EH) enzymes in the hydrolysis of skin-relevant fatty acid epoxides and comparison to the products to those of acid-catalyzed hydrolysis | Homo sapiens |
additional information | evaluation of different epoxide hydrolase (EH) enzymes in the hydrolysis of skin-relevant fatty acid epoxides and comparison to the products to those of acid-catalyzed hydrolysis | Mus musculus |
physiological function | activities of EPHX2 and EPHX3 in production of the linoleate triols detected as end products of the 12R-lipoxygenase pathway in the epidermis and functioning in formation of the mammalian water permeability barrier | Homo sapiens |
physiological function | activities of EPHX2 and EPHX3 in production of the linoleate triols detected as end products of the 12R-lipoxygenase pathway in the epidermis and functioning in formation of the mammalian water permeability barrier | Mus musculus |
physiological function | activities of EPHX2 and EPHX3 in production of the linoleate triols detected as end products of the 12R-LOX pathway in the epidermis and functioning in formation of the mammalian water permeability barrier | Homo sapiens |