Cloned (Comment) | Organism |
---|---|
recombinant expression of human soluble epoxide hydrolase (sEH) in insect cells via baculovirus transfection system | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1-(1-isobutyrylpiperidin-4-yl)-3-(4-(3-(trifluoromethyl)-3H-diazirin-3-yl)phenyl)urea | binding structure at the enzyme's active site, docking analysis and molecular dynamics simulations using the PDB ID 4JNC structure as template | Homo sapiens | |
1-[4-methyl-6-(methylamino)-1,3,5-triazin-2-yl]-N-[2-(trifluoromethyl)benzyl]piperidine-4-carboxamide | analysis of the crystal structure of enzyme-inhibitor complex | Homo sapiens | |
N-[4-(2-methylpropanoyl)cyclohexyl]-N'-[4-(trifluoromethyl)phenyl]urea | i.e. UC2389 | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Homo sapiens | 5829 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
14S,15R-epoxy-eicosatrienoic acid + H2O | Homo sapiens | - |
14S,15R-dihydroxy-eicosatrienoic acid | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P34913 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant soluble epoxide hydrolase (sEH) from insect cells by affinity chromatography | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(5Z,8Z)-N-((2-methoxy-4-(3-(trifluoromethyl)-3H-diazirin-3-yl)phenyl)sulfonyl)-10-(3-((Z)-oct-2-en-1-yl)oxiran-2-yl)deca-5,8-dienamide + H2O | - |
Homo sapiens | ? | - |
? | |
(5Z,8Z,11Z)-14,15-epoxyeicosa-5,8,11-trienoic acid + H2O | binding structure in the active site, docking analysis and molecular dynamics simulations | Homo sapiens | (5Z,8Z,11Z)-14,15-dihydroxyeicosa-5,8,11-trienoic acid | - |
? | |
(5Z,8Z,11Z)-N-((2-methoxy-4-(3-(trifluoromethyl)-3H-diazirin-3-yl)phenyl) sulfonyl)-13-(3-pentyloxiran-2-yl)trideca-5,8,11-trienamide + H2O | - |
Homo sapiens | ? | - |
? | |
11,12-epoxy-eicosatrienoic acid + H2O | - |
Homo sapiens | 11,12-dihydroxy-eicosatrienoic acid | - |
? | |
14S,15R-epoxy-eicosatrienoic acid + H2O | - |
Homo sapiens | 14S,15R-dihydroxy-eicosatrienoic acid | - |
? | |
trans-1,3-diphenylpropene oxide + H2O | - |
Homo sapiens | trans-1,3-diphenylpropane-1,2-diol | - |
? |
Synonyms | Comment | Organism |
---|---|---|
EPHX2 | - |
Homo sapiens |
SEH | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Homo sapiens |