Literature summary for 3.3.2.10 extracted from

Nelson, J.W.; Subrahmanyan, R.M.; Summers, S.A.; Xiao, X.; Alkayed, N.J.
Soluble epoxide hydrolase dimerization is required for hydrolase activity (2013), J. Biol. Chem., 288, 7697-7703.

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Protein Variants

Protein Variants	Comment	Organism
E254R	the mutant is almost inactive	Homo sapiens
E254R/R287E	the mutation reduces the enzyme hydrolase activity	Homo sapiens
R287E	the mutant is almost inactive	Homo sapiens
R287Q	the mutation reduces the enzyme hydrolase activity	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.00584	-	Epoxy Fluor 7	mutant enzyme E254R, at pH 7.4 and 37°C	Homo sapiens
0.00691	-	Epoxy Fluor 7	mutant enzyme E254R/R287E, at pH 7.4 and 37°C	Homo sapiens
0.00696	-	Epoxy Fluor 7	mutant enzyme R287E, at pH 7.4 and 37°C	Homo sapiens
0.01017	-	Epoxy Fluor 7	mutant enzyme R287Q, at pH 7.4 and 37°C	Homo sapiens
0.01508	-	Epoxy Fluor 7	wild type enzyme, at pH 7.4 and 37°C	Homo sapiens

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
soluble	-	Homo sapiens	-	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
80000	-	2 * 80000, SDS-PAGE, dimerization is required for enzymatic activity	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	P34913	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
Epoxy Fluor 7 + H2O	i.e. cyano(6-methoxy-2-naphthalenyl)methyl[(2,3)-3-phenyloxiranyl]methyl ester	Homo sapiens	?	-	?

Subunits

Subunits	Comment	Organism
homodimer	2 * 80000, SDS-PAGE, dimerization is required for enzymatic activity	Homo sapiens

Synonyms

Synonyms	Comment	Organism
SEH	-	Homo sapiens

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Release 2023.1 (January 2023)