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Literature summary for 3.3.2.10 extracted from
- Inhibition of the soluble epoxide hydrolase by tyrosine nitration (2009), J. Biol. Chem., 284, 28156-28163.
Application
| Application | Comment | Organism |
|---|---|---|
| drug development | the enzyme is a promising target for drug development | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| Y383F | site-directed mutagenesis, the mutant shows abolished sEH activity | Mus musculus |
| Y383F | site-directed mutagenesis, the mutant shows abolished sEH activity | Homo sapiens |
| Y466F | site-directed mutagenesis, the mutant shows abolished sEH activity | Mus musculus |
| Y466F | site-directed mutagenesis, the mutant shows abolished sEH activity | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 3-morpholinosydnonimine | i.e. SIN-1, a peroxonitrite generator, causes nitration on several tyrosine residues including Tyr383 and Tyr466. 1-adamantyl-3-cyclohexylurea in vitro decreases sensitivity to SIN-1 | Homo sapiens |  |
| 3-morpholinosydnonimine | i.e. SIN-1, a peroxonitrite generator, causes nitration on several tyrosine residues including Tyr383 and Tyr466. 1-adamantyl-3-cyclohexylurea in vitro decreases sensitivity to SIN-1 | Mus musculus | |
| peroxynitrite | causes nitration on several tyrosine residues including Tyr383 and Tyr466 | Homo sapiens | |
| peroxynitrite | causes nitration on several tyrosine residues including Tyr383 and Tyr466 | Mus musculus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Mus musculus | 5829 | - |
| cytosol | - |
Homo sapiens | 5829 | - |
| soluble | - |
Mus musculus | - |
- |
| soluble | - |
Homo sapiens | - |
- |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (5Z,8Z,11Z)-14,15-epoxyeicosatrienoic acid + H2O | Mus musculus | - |
(5Z,8Z,11Z)-14,15-dihydroxyeicosatrienoic acid | - |
? | |
| (5Z,8Z,11Z)-14,15-epoxyeicosatrienoic acid + H2O | Homo sapiens | - |
(5Z,8Z,11Z)-14,15-dihydroxyeicosatrienoic acid | - |
? | |
| (5Z,8Z,11Z)-14,15-epoxyeicosatrienoic acid + H2O | Mus musculus C57BL/6 | - |
(5Z,8Z,11Z)-14,15-dihydroxyeicosatrienoic acid | - |
? | |
| additional information | Mus musculus | activity of the sEH can be regulated by the tyrosine nitration of the protein | ? | - |
? | |
| additional information | Homo sapiens | activity of the sEH can be regulated by the tyrosine nitration of the protein | ? | - |
? | |
| additional information | Mus musculus C57BL/6 | activity of the sEH can be regulated by the tyrosine nitration of the protein | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | P34913 | gene EPHX2 | - |
| Mus musculus | P34914 | C57BL/6 mice | - |
| Mus musculus C57BL/6 | P34914 | C57BL/6 mice | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| endothelial cell | - |
Homo sapiens | - |
| endothelial cell | from lung | Mus musculus | - |
| HEK-293 cell | - |
Homo sapiens | - |
| HUVEC cell | - |
Homo sapiens | - |
| lung | - |
Mus musculus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (5Z,8Z,11Z)-14,15-epoxyeicosatrienoic acid + H2O | - |
Mus musculus | (5Z,8Z,11Z)-14,15-dihydroxyeicosatrienoic acid | - |
? | |
| (5Z,8Z,11Z)-14,15-epoxyeicosatrienoic acid + H2O | - |
Homo sapiens | (5Z,8Z,11Z)-14,15-dihydroxyeicosatrienoic acid | - |
? | |
| (5Z,8Z,11Z)-14,15-epoxyeicosatrienoic acid + H2O | - |
Mus musculus C57BL/6 | (5Z,8Z,11Z)-14,15-dihydroxyeicosatrienoic acid | - |
? | |
| additional information | activity of the sEH can be regulated by the tyrosine nitration of the protein | Mus musculus | ? | - |
? | |
| additional information | activity of the sEH can be regulated by the tyrosine nitration of the protein | Homo sapiens | ? | - |
? | |
| additional information | activity of the sEH can be regulated by the tyrosine nitration of the protein | Mus musculus C57BL/6 | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| SEH | - |
Mus musculus |
| SEH | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Mus musculus |
| 37 | - |
assay at | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.2 | - |
assay at | Mus musculus |
| 7.2 | - |
assay at | Homo sapiens |
General Information
| General Information | Comment | Organism |
|---|---|---|
| malfunction | in vivo, streptozotocin-induced diabetes results in the tyrosine nitration of the sEH in murine lungs and a significant decrease in its activity. Inhibition of sEH has beneficial effects on vascular inflammation and hypertension | Mus musculus |
| malfunction | inhibition of sEH has beneficial effects on vascular inflammation and hypertension | Homo sapiens |
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