Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.3.2.10 extracted from

  • Cronin, A.; Homburg, S.; Duerk, H.; Richter, I.; Adamska, M.; Frere, F.; Arand, M.
    Insights into the catalytic mechanism of human sEH phosphatase by site-directed mutagenesis and LC-MS/MS analysis (2008), J. Mol. Biol., 383, 627-640.
    View publication on PubMed
Search for more literature for 3.3.2.10

Cloned(Commentary)

Cloned (Comment) Organism
mutants are expressed in Escherichia coli BL21-AI cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
D11A inactive Homo sapiens
D11N mutant with strongly reduced phosphatase activity of sEH (2fold decrease in Km and 9fold decrease in kcat compared to the wild type enzyme), the N-terminal domain has phosphatase activity, the C-terminal domain has epoxide hydrolase activity Homo sapiens
D184A the mutation results in a complete loss of the phosphatase activity of sEH, the N-terminal domain has phosphatase activity, the C-terminal domain has epoxide hydrolase activity Homo sapiens
D184N the mutation results in a complete loss of the phosphatase activity of sEH, the N-terminal domain has phosphatase activity, the C-terminal domain has epoxide hydrolase activity Homo sapiens
D185A the mutation leads to a completely inactive sEH phosphatase, the N-terminal domain has phosphatase activity, the C-terminal domain has epoxide hydrolase activity Homo sapiens
D185A the mutation results in a complete loss of the phosphatase activity of sEH, the N-terminal domain has phosphatase activity, the C-terminal domain has epoxide hydrolase activity Homo sapiens
D185N the mutation reduces Vmax of the enzyme to 35% while leaving the Km unchanged, the N-terminal domain has phosphatase activity, the C-terminal domain has epoxide hydrolase activity Homo sapiens
D9A the mutation results in a complete loss of the phosphatase activity of sEH, the N-terminal domain has phosphatase activity, the C-terminal domain has epoxide hydrolase activity Homo sapiens
D9N the mutation results in a complete loss of the phosphatase activity of sEH, the N-terminal domain has phosphatase activity, the C-terminal domain has epoxide hydrolase activity Homo sapiens
K160A the mutation results in a complete loss of the phosphatase activity of sEH, the N-terminal domain has phosphatase activity, the C-terminal domain has epoxide hydrolase activity Homo sapiens
K160N the mutation results in a complete loss of the phosphatase activity of sEH, the N-terminal domain has phosphatase activity, the C-terminal domain has epoxide hydrolase activity Homo sapiens
K160R the mutation results in a complete loss of the phosphatase activity of sEH, the N-terminal domain has phosphatase activity, the C-terminal domain has epoxide hydrolase activity Homo sapiens
N123D the mutation results in a complete loss of the phosphatase activity of sEH, the N-terminal domain has phosphatase activity, the C-terminal domain has epoxide hydrolase activity Homo sapiens
N124D mutant with strongly reduced phosphatase activity of she, the N-terminal domain has phosphatase activity, the C-terminal domain has epoxide hydrolase activity Homo sapiens
N189A the mutation results in a complete loss of the phosphatase activity of sEH, the N-terminal domain has phosphatase activity, the C-terminal domain has epoxide hydrolase activity Homo sapiens
N189D the mutation results in a complete loss of the phosphatase activity of sEH, the N-terminal domain has phosphatase activity, the C-terminal domain has epoxide hydrolase activity Homo sapiens
T123A mutant with strongly reduced phosphatase activity of she, the N-terminal domain has phosphatase activity, the C-terminal domain has epoxide hydrolase activity Homo sapiens
T123N mutant with strongly reduced phosphatase activity of sEH, the N-terminal domain has phosphatase activity, the C-terminal domain has epoxide hydrolase activity Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
24000
-
 recombinant enzyme, SDS-PAGE Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens P34913
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography and gel filtration Homo sapiens

Synonyms

Synonyms Comment Organism
EC 3.1.3.76
-
 Homo sapiens
SEH bifunctional enzyme, shows also phosphatase activity Homo sapiens