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Literature summary for 3.3.2.10 extracted from

  • Harris, T.R.; Aronov, P.A.; Hammock, B.D.
    Soluble epoxide hydrolase homologs in Strongylocentrotus purpuratus suggest a gene duplication event and subsequent divergence (2008), DNA Cell Biol., 27, 467-477.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
cDNA extraction, RACE PCR with RNA-fragment of soluble epoxide hydrolase-like protein 1 (SPEH1), insertion into baculovirus transfection vector pACUW21 or pBacPAK8, recombinant baculoviruses generated by cotransfection of Spodoptera frugiperda Sf21 cells with described vector and Bsu361-cleaved BacPAK6 viral DNA, infection of Trichoplusia ni High Five cells Strongylocentrotus purpuratus
cDNA extraction, RACE PCR with RNA-fragment of soluble epoxide hydrolase-like protein 2 (SPEH2), insertion into baculovirus transfection vector pACUW21 or pBacPAK8, recombinant baculoviruses generated by cotransfection of Spodoptera frugiperda Sf21 cells with described vector and Bsu361-cleaved BacPAK6 viral DNA, infection of Trichoplusia ni High Five cells Strongylocentrotus purpuratus

Inhibitors

Inhibitors Comment Organism Structure
12-(3-adamantane-1-yl-ureido)-dodecanoic acid
-
Strongylocentrotus purpuratus
N,N'-dicyclohexylurea
-
Strongylocentrotus purpuratus
N-adamantyl-N'-cyclohexylurea
-
Strongylocentrotus purpuratus
N-cyclohexyl(dodecylamino)carboxamide
-
Strongylocentrotus purpuratus
N-cyclohexyl-N'-ethylurea
-
Strongylocentrotus purpuratus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information SPEH1 displays no activity on substrate 2'-(2-benzothiazolyl)-6'-hydroxybenzothiazole phosphate, substrate used to assay human sEH N-terminal phosphatise activity Strongylocentrotus purpuratus
0.035
-
trans-diphenyl-propene oxide
-
Strongylocentrotus purpuratus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
63800
-
predicted: 63600, SPEH1 Strongylocentrotus purpuratus
64200
-
predicted: 64400, SPEH2 Strongylocentrotus purpuratus

Organism

Organism UniProt Comment Textmining
Strongylocentrotus purpuratus B2MWN2
-
-
Strongylocentrotus purpuratus B2MWN3
-
-

Purification (Commentary)

Purification (Comment) Organism
Trichoplusia ni High Five cells homogenization by Ultra Turrax, talon metal affinity resin, concentration in a 30000 Da cut Centricon centrifugal filter unit, desalting with desalting column Strongylocentrotus purpuratus

Source Tissue

Source Tissue Comment Organism Textmining
gonad
-
Strongylocentrotus purpuratus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.033
-
(5Z,11Z,14Z)-8,9-epoxyeicosa-8,11,14-trienoic acid Strongylocentrotus purpuratus
0.33
-
(5Z,8Z,11Z)-14,15-epoxyeicosa-5,8,11-trienoic acid Strongylocentrotus purpuratus
0.49
-
(5Z,8Z,13Z)-11,12-epoxyeicosa-5,8,14-trienoic acid Strongylocentrotus purpuratus
3.1
-
trans-1,3-diphenylpropene oxide Strongylocentrotus purpuratus
4.2
-
(9Z)-12,13-epoxyoctadec-9-enoic acid Strongylocentrotus purpuratus
4.8
-
(12Z)-9,10-epoxyoctadec-12-enoic acid Strongylocentrotus purpuratus

Storage Stability

Storage Stability Organism
SPEH1 EH activity displays a half-life of 2 h at 37°C, of over 1 week at 4°C and of 1-2 days at room temperature Strongylocentrotus purpuratus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(12Z)-9,10-epoxyoctadec-12-enoic acid
-
Strongylocentrotus purpuratus (12Z)-9,10-dihydroxyoctadec-12-enoic acid
-
?
(5Z,11Z,14Z)-8,9-epoxyeicosa-8,11,14-trienoic acid + H2O
-
Strongylocentrotus purpuratus (5Z,11Z,14Z)-8,9-dihydroxyeicosa-5,11,14-trienoic acid
-
?
(5Z,8Z,11Z)-14,15-epoxyeicosa-5,8,11-trienoic acid + H2O
-
Strongylocentrotus purpuratus (5Z,8Z,11Z)-14,15-dihydroxyeicosa-5,8,11-trienoic acid
-
?
(5Z,8Z,14Z)-11,12-epoxyeicosa-5,8,14-trienoic acid + H2O
-
Strongylocentrotus purpuratus (5Z,8Z,14Z)-11,12-dihydroxyeicosa-5,8,14-trienoic acid
-
?
(9Z)-12,13-epoxyoctadec-9-enoic acid + H2O
-
Strongylocentrotus purpuratus (9Z)-12,13-dihydroxyoctadec-9-enoic acid
-
?
trans-1,3-diphenylpropene oxide + H2O tritium labeled for radiometric assay Strongylocentrotus purpuratus 1,3-diphenylpropane-1,2-diol
-
?
trans-diphenyl-propene oxide + H2O
-
Strongylocentrotus purpuratus ?
-
?

Synonyms

Synonyms Comment Organism
SPEH1 epoxide hydrolase Strongylocentrotus purpuratus
SPEH2 epoxide hydrolase Strongylocentrotus purpuratus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
SPEH1 EH activity displays a half-life of 1-2 days at room temperature Strongylocentrotus purpuratus
4
-
SPEH1 EH activity displays a half-life of over one week Strongylocentrotus purpuratus
37
-
SPEH1 EH activity displays a half-life of 2 h Strongylocentrotus purpuratus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
44
-
trans-1,3-diphenylpropene oxide
-
Strongylocentrotus purpuratus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
for SPEH1 epxide hydrolase activity Strongylocentrotus purpuratus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
additional information
-
additional information SPEH1 displays the same pattern of inhibition as the nematode soluble epoxide hydrolase homolog CEEH1 Strongylocentrotus purpuratus

pI Value

Organism Comment pI Value Maximum pI Value
Strongylocentrotus purpuratus to 5.9 range, crude extracts from gonads, supernatant
-
5.7
Strongylocentrotus purpuratus to 6.1 range, recombinant SPEH1, His6-tagged
-
5.9

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.000057
-
recombinant SPEH1 Strongylocentrotus purpuratus 12-(3-adamantane-1-yl-ureido)-dodecanoic acid
0.000059
-
crude extract from gonads Strongylocentrotus purpuratus 12-(3-adamantane-1-yl-ureido)-dodecanoic acid
0.00069
-
crude extract from gonads Strongylocentrotus purpuratus N-cyclohexyl(dodecylamino)carboxamide
0.00091
-
recombinant SPEH1 Strongylocentrotus purpuratus N-cyclohexyl(dodecylamino)carboxamide
0.0023
-
recombinant SPEH1 Strongylocentrotus purpuratus N-adamantyl-N'-cyclohexylurea
0.05
-
crude extract from gonads Strongylocentrotus purpuratus N-cyclohexyl-N'-ethylurea
0.05
-
more than the value, recombinant SPEH1 Strongylocentrotus purpuratus N,N'-dicyclohexylurea
0.05
-
more than the value, recombinant SPEH1 Strongylocentrotus purpuratus N-cyclohexyl-N'-ethylurea