Cloned (Comment) | Organism |
---|---|
cDNA extraction, RACE PCR with RNA-fragment of soluble epoxide hydrolase-like protein 1 (SPEH1), insertion into baculovirus transfection vector pACUW21 or pBacPAK8, recombinant baculoviruses generated by cotransfection of Spodoptera frugiperda Sf21 cells with described vector and Bsu361-cleaved BacPAK6 viral DNA, infection of Trichoplusia ni High Five cells | Strongylocentrotus purpuratus |
cDNA extraction, RACE PCR with RNA-fragment of soluble epoxide hydrolase-like protein 2 (SPEH2), insertion into baculovirus transfection vector pACUW21 or pBacPAK8, recombinant baculoviruses generated by cotransfection of Spodoptera frugiperda Sf21 cells with described vector and Bsu361-cleaved BacPAK6 viral DNA, infection of Trichoplusia ni High Five cells | Strongylocentrotus purpuratus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
12-(3-adamantane-1-yl-ureido)-dodecanoic acid | - |
Strongylocentrotus purpuratus | |
N,N'-dicyclohexylurea | - |
Strongylocentrotus purpuratus | |
N-adamantyl-N'-cyclohexylurea | - |
Strongylocentrotus purpuratus | |
N-cyclohexyl(dodecylamino)carboxamide | - |
Strongylocentrotus purpuratus | |
N-cyclohexyl-N'-ethylurea | - |
Strongylocentrotus purpuratus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | SPEH1 displays no activity on substrate 2'-(2-benzothiazolyl)-6'-hydroxybenzothiazole phosphate, substrate used to assay human sEH N-terminal phosphatise activity | Strongylocentrotus purpuratus | |
0.035 | - |
trans-diphenyl-propene oxide | - |
Strongylocentrotus purpuratus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
63800 | - |
predicted: 63600, SPEH1 | Strongylocentrotus purpuratus |
64200 | - |
predicted: 64400, SPEH2 | Strongylocentrotus purpuratus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Strongylocentrotus purpuratus | B2MWN2 | - |
- |
Strongylocentrotus purpuratus | B2MWN3 | - |
- |
Purification (Comment) | Organism |
---|---|
Trichoplusia ni High Five cells homogenization by Ultra Turrax, talon metal affinity resin, concentration in a 30000 Da cut Centricon centrifugal filter unit, desalting with desalting column | Strongylocentrotus purpuratus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
gonad | - |
Strongylocentrotus purpuratus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.033 | - |
(5Z,11Z,14Z)-8,9-epoxyeicosa-8,11,14-trienoic acid | Strongylocentrotus purpuratus |
0.33 | - |
(5Z,8Z,11Z)-14,15-epoxyeicosa-5,8,11-trienoic acid | Strongylocentrotus purpuratus |
0.49 | - |
(5Z,8Z,13Z)-11,12-epoxyeicosa-5,8,14-trienoic acid | Strongylocentrotus purpuratus |
3.1 | - |
trans-1,3-diphenylpropene oxide | Strongylocentrotus purpuratus |
4.2 | - |
(9Z)-12,13-epoxyoctadec-9-enoic acid | Strongylocentrotus purpuratus |
4.8 | - |
(12Z)-9,10-epoxyoctadec-12-enoic acid | Strongylocentrotus purpuratus |
Storage Stability | Organism |
---|---|
SPEH1 EH activity displays a half-life of 2 h at 37°C, of over 1 week at 4°C and of 1-2 days at room temperature | Strongylocentrotus purpuratus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(12Z)-9,10-epoxyoctadec-12-enoic acid | - |
Strongylocentrotus purpuratus | (12Z)-9,10-dihydroxyoctadec-12-enoic acid | - |
? | |
(5Z,11Z,14Z)-8,9-epoxyeicosa-8,11,14-trienoic acid + H2O | - |
Strongylocentrotus purpuratus | (5Z,11Z,14Z)-8,9-dihydroxyeicosa-5,11,14-trienoic acid | - |
? | |
(5Z,8Z,11Z)-14,15-epoxyeicosa-5,8,11-trienoic acid + H2O | - |
Strongylocentrotus purpuratus | (5Z,8Z,11Z)-14,15-dihydroxyeicosa-5,8,11-trienoic acid | - |
? | |
(5Z,8Z,14Z)-11,12-epoxyeicosa-5,8,14-trienoic acid + H2O | - |
Strongylocentrotus purpuratus | (5Z,8Z,14Z)-11,12-dihydroxyeicosa-5,8,14-trienoic acid | - |
? | |
(9Z)-12,13-epoxyoctadec-9-enoic acid + H2O | - |
Strongylocentrotus purpuratus | (9Z)-12,13-dihydroxyoctadec-9-enoic acid | - |
? | |
trans-1,3-diphenylpropene oxide + H2O | tritium labeled for radiometric assay | Strongylocentrotus purpuratus | 1,3-diphenylpropane-1,2-diol | - |
? | |
trans-diphenyl-propene oxide + H2O | - |
Strongylocentrotus purpuratus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
SPEH1 | epoxide hydrolase | Strongylocentrotus purpuratus |
SPEH2 | epoxide hydrolase | Strongylocentrotus purpuratus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
SPEH1 EH activity displays a half-life of 1-2 days at room temperature | Strongylocentrotus purpuratus |
4 | - |
SPEH1 EH activity displays a half-life of over one week | Strongylocentrotus purpuratus |
37 | - |
SPEH1 EH activity displays a half-life of 2 h | Strongylocentrotus purpuratus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
44 | - |
trans-1,3-diphenylpropene oxide | - |
Strongylocentrotus purpuratus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
for SPEH1 epxide hydrolase activity | Strongylocentrotus purpuratus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | SPEH1 displays the same pattern of inhibition as the nematode soluble epoxide hydrolase homolog CEEH1 | Strongylocentrotus purpuratus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Strongylocentrotus purpuratus | to 5.9 range, crude extracts from gonads, supernatant | - |
5.7 |
Strongylocentrotus purpuratus | to 6.1 range, recombinant SPEH1, His6-tagged | - |
5.9 |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.000057 | - |
recombinant SPEH1 | Strongylocentrotus purpuratus | 12-(3-adamantane-1-yl-ureido)-dodecanoic acid | |
0.000059 | - |
crude extract from gonads | Strongylocentrotus purpuratus | 12-(3-adamantane-1-yl-ureido)-dodecanoic acid | |
0.00069 | - |
crude extract from gonads | Strongylocentrotus purpuratus | N-cyclohexyl(dodecylamino)carboxamide | |
0.00091 | - |
recombinant SPEH1 | Strongylocentrotus purpuratus | N-cyclohexyl(dodecylamino)carboxamide | |
0.0023 | - |
recombinant SPEH1 | Strongylocentrotus purpuratus | N-adamantyl-N'-cyclohexylurea | |
0.05 | - |
crude extract from gonads | Strongylocentrotus purpuratus | N-cyclohexyl-N'-ethylurea | |
0.05 | - |
more than the value, recombinant SPEH1 | Strongylocentrotus purpuratus | N,N'-dicyclohexylurea | |
0.05 | - |
more than the value, recombinant SPEH1 | Strongylocentrotus purpuratus | N-cyclohexyl-N'-ethylurea |