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Literature summary for 3.3.2.10 extracted from

  • Cronin, A.; Mowbray, S.; Duerk, H.; Homburg, S.; Fleming, I.; Fisslthaler, B.; Oesch, F.; Arand, M.
    The N-terminal domain of mammalian soluble epoxide hydrolase is a phosphatase (2003), Proc. Natl. Acad. Sci. USA, 100, 1552-1557.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.3.2.10

Cloned(Commentary)

Cloned (Comment) Organism
expression of His-tagged wild-type and mutant enzymes from cDNA in Escherichia coli strain BL21-AI, expression of strep-tagged enzyme from full-length gene in Escherichia coli strain XL1-Blue Homo sapiens
expression of the enzyme from full-length gene in Escherichia coli strain BL21-AI Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
4-fluorochalcone oxide inhibition of the epoxide hydrolase activity Rattus norvegicus
dicyclohexylurea inhibition of the epoxide hydrolase activity Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.003
-
 trans-stilbene oxide native enzyme Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Homo sapiens 5829
-
cytosol
-
 Rattus norvegicus 5829
-
cytosol
-
 Mus musculus 5829
-

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Mus musculus P34914
-
-
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme from liver to homogeneity by ultracentrifugation and affinity chromatography on a benzyl thio resin using 4-fluorochalcone oxide as eluent Rattus norvegicus
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21-AI by nickel affinity chromatography, recombinant strep-tagged wild-type enzyme from Escherichia coli strain XL1-Blue by streptavidin affinity chromatography Homo sapiens

Reaction

Reaction Comment Organism Reaction ID
an epoxide + H2O = a glycol the C-terminal part harbors the epoxide hydrolase activity, the phosphatase activity of the enzyme is located at the N-terminal part of EC 3.1.3.76, both catalytic sites act independently Homo sapiens
a
an epoxide + H2O = a glycol the C-terminal part harbors the epoxide hydrolase activity, the phosphatase activity of the enzyme is located at the N-terminal part of EC 3.1.3.76, both catalytic sites act independently Rattus norvegicus
a
an epoxide + H2O = a glycol the C-terminal part harbors the epoxide hydrolase activity, the phosphatase activity of the enzyme is located at the N-terminal part of EC 3.1.3.76, both catalytic sites act independently Mus musculus
a

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Homo sapiens
-
liver
-
 Rattus norvegicus
-
liver
-
 Mus musculus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
trans-stilbene oxide + H2O
-
 Homo sapiens ?
-
 ?
trans-stilbene oxide + H2O
-
 Rattus norvegicus ?
-
 ?
trans-stilbene oxide + H2O
-
 Mus musculus ?
-
 ?

Subunits

Subunits Comment Organism
dimer
-
 Homo sapiens
dimer
-
 Rattus norvegicus
dimer
-
 Mus musculus
More the 25 kDa C-terminal part, with a different hydrolase alpha/beta-fold, harbors the epoxide hydrolase activity, the enzyme's phosphatase activity of EC3.1.3.76 is located at the 35 kDa N-terminal part which has a alpha/beta-fold, structure analysis Homo sapiens
More the 25 kDa C-terminal part, with a different hydrolase alpha/beta-fold, harbors the epoxide hydrolase activity, the enzyme's phosphatase activity of EC3.1.3.76 is located at the 35 kDa N-terminal part which has a alpha/beta-fold, structure analysis Rattus norvegicus
More the 25 kDa C-terminal part, with a different hydrolase alpha/beta-fold, harbors the epoxide hydrolase activity, the enzyme's phosphatase activity of EC3.1.3.76 is located at the 35 kDa N-terminal part which has a alpha/beta-fold, structure analysis Mus musculus

Synonyms

Synonyms Comment Organism
SEH
-
 Homo sapiens
SEH
-
 Rattus norvegicus
SEH
-
 Mus musculus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.5
-
 trans-stilbene oxide native enzyme Rattus norvegicus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.00003
-
 dicyclohexylurea native enzyme Rattus norvegicus
0.0005
-
 4-fluorochalcone oxide native enzyme Rattus norvegicus