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Literature summary for 3.3.2.10 extracted from

  • Reetz, M.T.; Torre, C.; Eipper, A.; Lohmer, R.; Hermes, M.; Brunner, B.; Maichele, A.; Bocola, M.; Arand, M.; Cronin, A.; Genzel, Y.; Archelas, A.; Furstoss, R.
    Enhancing the enantioselectivity of an epoxide hydrolase by directed evolution (2004), Org. Lett., 6, 177-180.
    View publication on PubMed
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Protein Variants

Protein Variants Comment Organism
additional information the selectivity factor E reflecting the relative rate of the reaction of the two enantiomers is only 4.6 for the wild-type enzyme, in slight favor of the (S)-product, a value of 10.8 is observed with the natural EH variant IS002B1 with the three amino acid exchanges A217V, K332E and A390E. A value of 7.4 is observed with the natural variant IR003B1 with the amino acid exchange R219G, a value of 6.6 is observed with the natural variant IB001C2 with the amino acid exchange F340Y, a value of 6.4 is observed with the natural variant IE001H6 with the amino acid exchange A327V, a value of 6.2 is observed with the natural variant IS001H8 with the amino acid exchange A327V and a value of 5.5 is observed with the natural variant IL001D4 with the amino acid exchange P222S Aspergillus niger

Organism

Organism UniProt Comment Textmining
Aspergillus niger
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glycidyl phenyl ether + H2O the selectivity factor E reflecting the relative rate of the reaction of the two enantiomers is only 4.6 for the wild-type enzyme, in slight favor of the (S)-product, a value of 10.8 is observed with the natural EH variant IS002B1 with the three amino acid exchanges A217V, K332E and A390E. A value of 7.4 is observed with the natural variant IR003B1 with the amino acid exchange R219G, a value of 6.6 is observed with the natural variant IB001C2 with the amino acid exchange F340Y, a value of 6.4 is observed with the natural variant IE001H6 with the amino acid exchange A327V, a value of 6.2 is observed with the natural variant IS001H8 with the amino acid exchange A327V and a value of 5.5 is observed with the natural variant IL001D4 with the amino acid exchange P222S Aspergillus niger ?
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