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Literature summary for 3.3.2.10 extracted from
- Stereochemical features of the hydrolysis of 9,10-epoxystearic acid catalysed by plant and mammalian epoxide hydrolases (2002), Biochem. J., 366, 471-480.
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| E404D | site-directed mutagenesis, the mutant enzyme shows unaltered enantioselectivity with stearic acid oxide substrate compared to the wild-type enzyme | Rattus norvegicus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetics | Rattus norvegicus |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| microsome | - |
Rattus norvegicus | - |
- |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| an epoxide + H2O = a glycol | reaction mechanism, catalytic triad | Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Rattus norvegicus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cis-(9R,10S)-epoxystearic acid + H2O | - |
Rattus norvegicus | threo-(9R,10R)-dihydroxystearic acid + threo-(9S,10S)-dihydroxystearic acid | enantioselective, low production rate of the (9R,10R)-diol by wild-type and E404D mutant | ? |  |
| cis-(9S,10R)-epoxystearic acid + H2O | - |
Rattus norvegicus | threo-(9R,10R)-dihydroxystearic acid + threo-(9S,10S)-dihydroxystearic acid | enantioselective, low production rate of the (9R,10R)-diol by wild-type and E404D mutant | ? | |
| additional information | enantioselectivity with fatty acid epoxide substrates | Rattus norvegicus | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| mEH | - |
Rattus norvegicus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 26 | - |
assay at | Rattus norvegicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
assay at | Rattus norvegicus |
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Release 2023.1 (January 2023)
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