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Literature summary for 3.3.2.10 extracted from

  • Morisseau, C.; Archelas, A.; Guitton, C.; Faucher, D.; Furstoss, R.; Baratti, J.C.
    Purification and characterization of a highly enantioselective epoxide hydrolase from Aspergillus niger (1999), Eur. J. Biochem., 263, 386-395.
    View publication on PubMed
Search for more literature for 3.3.2.10

Application

Application Comment Organism
synthesis the enzyme may be a good biocatalyst for the preparation of enantiopure epoxides or diols Aspergillus niger

Inhibitors

Inhibitors Comment Organism Structure
4-hydroxymercuribenzoate
-
 Aspergillus niger
cis-limonene epoxide weak Aspergillus niger
dithionitrobenzene
-
 Aspergillus niger
epichlorhydrin weak Aspergillus niger
geraniol derivatives weak Aspergillus niger
H2O2
-
 Aspergillus niger
HgCl2
-
 Aspergillus niger
iodoacetamide
-
 Aspergillus niger
m-chloroperbenzoic acid
-
 Aspergillus niger
omega-bromo-nitro-acetophenone
-
 Aspergillus niger
trans-limonene epoxide weak Aspergillus niger
trichloropropene oxide
-
 Aspergillus niger

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.6
-
 (R)-p-nitrostyrene oxide
-
 Aspergillus niger
1
-
 (R,S)-4-nitrostyrene oxide
-
 Aspergillus niger
3.7
-
 (S)-4-nitrostyrene oxide
-
 Aspergillus niger

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
 4 * 45000, SDS-PAGE Aspergillus niger
185000
-
 gel filtration Aspergillus niger

Organism

Organism UniProt Comment Textmining
Aspergillus niger
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Aspergillus niger

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.102
-
-
 Aspergillus niger

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R)-p-nitrostyrene oxide + H2O
-
 Aspergillus niger 1-(4-nitrophenyl)ethane-1,2-diol
-
 ?
(R,S)-4-nitrostyrene oxide + H2O
-
 Aspergillus niger 1-(4-nitrophenyl)ethane-1,2-diol
-
 ?
(S)-4-nitrostyrene oxide
-
 Aspergillus niger 1-(4-nitrophenyl)ethane-1,2-diol
-
 ?
p-nitrostyrene oxide + H2O
-
 Aspergillus niger ?
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 45000, SDS-PAGE Aspergillus niger

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Aspergillus niger

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
15 45 15°C: about 50% of maximal activity, 45°C: about 65% of maximal activity Aspergillus niger

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.7
-
 (S)-4-nitrostyrene oxide
-
 Aspergillus niger
13.9
-
 (R,S)-4-nitrostyrene oxide
-
 Aspergillus niger
16.4
-
 (R)-p-nitrostyrene oxide
-
 Aspergillus niger

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Aspergillus niger

pH Range

pH Minimum pH Maximum Comment Organism
5.5 8 pH 5.5: about 35% of maximal activity, pH 8.0: about 45% of maximal activity Aspergillus niger