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Literature summary for 3.3.2.10 extracted from
- Purification of human liver cytosolic epoxide hydrolase and comparison to the microsomal enzyme (1982), Biochemistry, 21, 5769-5776.
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Homo sapiens | 5829 | - |
| cytosol | - |
Rattus norvegicus | 5829 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 50000 | - |
x * 50000, SDS-PAGE | Rattus norvegicus |
| 58000 | - |
gel filtration, monomeric or dimeric enzyme, dependent on conditions | Homo sapiens |
| 140000 | - |
gel filtration, monomeric or dimeric enzyme, dependent on conditions | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
| Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 425fold from liver cytosol to homogeneity by ammonium sulfate fractionation, cellulose resin, hydroxylapaptite, and ion exchange chromatography | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Homo sapiens | - |
| liver | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
substrate specificity | Rattus norvegicus |
| additional information | - |
substrate specificity, 2 enzymes with different specificities termed cytosolic TESO hydrolase and cytosolic PNSO hydrolase | Homo sapiens |
| 0.0078 | - |
purified enzyme, substrate 4-nitrostyrene 7,8-oxide | Homo sapiens |
| 0.051 | - |
purified enzyme, substrate trans-8-ethylstyrene 7,8-oxide | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrostyrene 7,8-oxide + H2O | i.e. PNSO | Homo sapiens | ? | - |
? |  |
| 4-nitrostyrene 7,8-oxide + H2O | i.e. PNSO | Rattus norvegicus | ? | - |
? | |
| cis-8-ethylstyrene 7,8-oxide + H2O | - |
Homo sapiens | ? | - |
? | |
| cis-8-ethylstyrene 7,8-oxide + H2O | - |
Rattus norvegicus | ? | - |
? | |
| additional information | substrate specificity, 2 enzymes with different specificities termed cytosolic TESO hydrolase and cytosolic PNSO hydrolase, no activity with benz[a]pyrene 4,5-oxide | Homo sapiens | ? | - |
? | |
| additional information | substrate specificity, no activity with benz[a]pyrene 4,5-oxide | Rattus norvegicus | ? | - |
? | |
| naphthalene 1,2-oxide + H2O | - |
Homo sapiens | ? | - |
? | |
| naphthalene 1,2-oxide + H2O | - |
Rattus norvegicus | ? | - |
? | |
| styrene 7,8-oxide + H2O | - |
Homo sapiens | ? | - |
? | |
| styrene 7,8-oxide + H2O | - |
Rattus norvegicus | ? | - |
? | |
| trans-8-ethylstyrene 7,8-oxide + H2O | i.e. TESO | Homo sapiens | ? | - |
? | |
| trans-8-ethylstyrene 7,8-oxide + H2O | i.e. TESO | Rattus norvegicus | ? | - |
? | |
| trans-stilbene oxide + H2O | - |
Homo sapiens | ? | - |
? | |
| trans-stilbene oxide + H2O | - |
Rattus norvegicus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 50000, SDS-PAGE | Rattus norvegicus |
| monomer or dimer | n * 58000, SDS-PAGE | Homo sapiens |
| More | peptide mapping | Homo sapiens |
| More | peptide mapping | Rattus norvegicus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PNSO hydrolase | - |
Homo sapiens |
| TESO hydrolase | - |
Homo sapiens |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Homo sapiens | isoelectric focusing | 6.1 | 5.1 |
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Release 2023.1 (January 2023)
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