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Literature summary for 3.3.2.1 extracted from

  • Rusnak, F.; Liu, J.; Quinn, N.; Berchtold, G.A.; Walsh, C.T.
    Subcloning of the enterobactin biosynthetic gene entB: expression, purification, characterization, and substrate specificity of isochorismatase (1990), Biochemistry, 29, 1425-1435.
    View publication on PubMed
Search for more literature for 3.3.2.1

Cloned(Commentary)

Cloned (Comment) Organism
-
 Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
(+/-)-3-[(carboxyethenyl)oxy]-6-hydroxy-1-benzoic acid i.e. 5-(1-carboxyvinyloxy)-2-hydroxybenzoic acid Escherichia coli
3-[(carboxyethenyl)oxy]benzoic acid i.e. 3-(1-carboxyvinyloxy)benzoic acid Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.015
-
 isochorismate
-
 Escherichia coli
0.023
-
 (+/-)-3-[(1-carboxyethenyl)oxy]-2-hydroxy-6-cyclohexene-1-carboxylic acid
-
 Escherichia coli
0.086
-
 (+/-)-3-[(carboxyethenyl)oxy]-1-cyclohexene-1-carboxylic acid
-
 Escherichia coli
0.12
-
 (+/-)-3-[(1-carboxyethenyl)oxy]-cyclohepta-1,6-diene-1-carboxylate
-
 Escherichia coli
0.28
-
 (+/-)-cis-3-[(carboxyethenyl)oxy]-4-cyclohexene-1-carboxylic acid
-
 Escherichia coli
35
-
 chorismate
-
 Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
 5 * 35000, SDS-PAGE Escherichia coli
159000
-
 gel filtration Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
isochorismate + H2O Escherichia coli biosynthesis of enterobactin 2,3-dihydroxy-2,3-dihydrobenzoate + pyruvate
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Escherichia coli

Storage Stability

Storage Stability Organism
-20°C, 25 mM Tris buffer, pH 8.0, 5 mM dithiothreitol, 50% glycerol, stable for several months Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(+/-)-3-[(1-carboxyethenyl)oxy]-2-hydroxy-6-cyclohexene-1-carboxylic acid + H2O
-
 Escherichia coli 3,4-dihydroxy-cyclohex-1-enecarboxylic acid + pyruvate
-
 ?
(+/-)-3-[(1-carboxyethenyl)oxy]-cyclohepta-1,6-diene-1-carboxylate + H2O i.e. (S)-3-(1-carboxyvinyloxy)-cyclohepta-1,6-dienecarboxylic acid Escherichia coli (S)-3-hydroxy-cyclohepta-1,6-dienecarboxylic acid + pyruvate
-
 ?
(+/-)-3-[(carboxyethenyl)oxy]-1-cyclohexene-1-carboxylic acid + H2O
-
 Escherichia coli (S)-3-hydroxycyclohex-1-enecarboxylic acid + pyruvate
-
 ?
(+/-)-cis-3-[(carboxyethenyl)oxy]-4-cyclohexene-1-carboxylic acid + H2O
-
 Escherichia coli (R)-5-hydroxycyclohex-3-enecarboxylic acid + pyruvate
-
 ?
chorismate + H2O slow hydrolysis Escherichia coli 3,4-dihydroxy-cyclohexa-1,5-dienecarboxylic acid + pyruvate
-
 ?
isochorismate + H2O
-
 Escherichia coli 2,3-dihydroxy-2,3-dihydrobenzoate + pyruvate
-
 ?
isochorismate + H2O biosynthesis of enterobactin Escherichia coli 2,3-dihydroxy-2,3-dihydrobenzoate + pyruvate
-
 ?

Subunits

Subunits Comment Organism
pentamer 5 * 35000, SDS-PAGE Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.17
-
 (+/-)-3-[(1-carboxyethenyl)oxy]-2-hydroxy-6-cyclohexene-1-carboxylic acid
-
 Escherichia coli
9
-
 (+/-)-3-[(1-carboxyethenyl)oxy]-cyclohepta-1,6-diene-1-carboxylate
-
 Escherichia coli
10
-
 isochorismate
-
 Escherichia coli
16.3
-
 (+/-)-cis-3-[(carboxyethenyl)oxy]-4-cyclohexene-1-carboxylic acid
-
 Escherichia coli
21.7
-
 (+/-)-3-[(carboxyethenyl)oxy]-1-cyclohexene-1-carboxylic acid
-
 Escherichia coli
217
-
 chorismate
-
 Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 7.5
-
 Escherichia coli