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Literature summary for 3.2.2.9 extracted from

  • Han, T.; Li, Y.; Shan, Q.; Liang, W.; Hao, W.; Li, Y.; Tan, X.; Gu, J.
    Characterization of S-adenosylhomocysteine/Methylthioadenosine nucleosidase on secretion of AI-2 and biofilm formation of Escherichia coli (2017), Microb. Pathog., 108, 78-84 .
    View publication on PubMed
Search for more literature for 3.2.2.9

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Escherichia coli
expression in Escherichia coli Mycobacterium tuberculosis

Crystallization (Commentary)

Crystallization (Comment) Organism
molecular modeling of structure and docking of inhibitors Mycobacterium tuberculosis

Inhibitors

Inhibitors Comment Organism Structure
(3R,4S)-1-[(4-amino-5H-pyrrolo[3,2-d]pyrimidin-7-yl)methyl]-4-methylpyrrolidin-3-ol most potent inhibitor tested, 5'-deoxyadenosine transition state analogue, dissociation constant of 640 pM. Inhibitor has a weak cell growth effect on Mycobacterium tuberculosis or Mycobacterium smegmatis but is lethal toward Helicobacter pylori Mycobacterium tuberculosis
(3R,4S)-1-[(4-amino-5H-pyrrolo[3,2-d]pyrimidin-7-yl)methyl]-4-[(hexylsulfanyl)methyl]pyrrolidin-3-ol dissociation constant of 87 pM, inhibitor has a weak cell growth effect on Mycobacterium tuberculosis or Mycobacterium smegmatis but is lethal toward Helicobacter pylori Mycobacterium tuberculosis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00173
-
 5'-methylthioadenosine 37°C, pH 7.0 Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli P0AF12
-
-
Mycobacterium tuberculosis P9WJM3
-
-
Mycobacterium tuberculosis H37Rv P9WJM3
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5'-deoxyadenosine + H2O
-
 Mycobacterium tuberculosis 5-deoxy-D-ribose + adenine
-
 ?
5'-deoxyadenosine + H2O
-
 Mycobacterium tuberculosis H37Rv 5-deoxy-D-ribose + adenine
-
 ?
5'-methylthioadenosine + H2O
-
 Escherichia coli 5-methylthio-D-ribose + adenine
-
 ?

Synonyms

Synonyms Comment Organism
MtnN
-
 Escherichia coli
MtnN
-
 Mycobacterium tuberculosis
SAHN
-
 Escherichia coli

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.00000064
-
 (3R,4S)-1-[(4-amino-5H-pyrrolo[3,2-d]pyrimidin-7-yl)methyl]-4-methylpyrrolidin-3-ol pH 7.4, 25°C Mycobacterium tuberculosis

General Information

General Information Comment Organism
physiological function deletion of the SAHN gene abolishes the production of the quorum sensing signal AI-2 and biofilm formation Escherichia coli