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Literature summary for 3.2.2.9 extracted from

  • Thomas, K.; Cameron, S.A.; Almo, S.C.; Burgos, E.S.; Gulab, S.A.; Schramm, V.L.
    Active site and remote contributions to catalysis in methylthioadenosine nucleosidases (2015), Biochemistry, 54, 2520-2529 .
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

Crystallization (Comment) Organism
in complex with transition state analogues Vibrio cholerae

Protein Variants

Protein Variants Comment Organism
A113P mutation of Vibrio cholera MTAN catalytic site residue to match those of Escherichia coli MTAN, about 4fold decrease in catalytic efficiency Vibrio cholerae
A113P/V153I mutation of Vibrio cholera MTAN catalytic site residues to match those of Escherichia coli MTAN. In the Val153Ile mutant or double mutants, transition-state analogue binding at one site causes complete inhibition. Amino acid, Val153, alters the catalytic site cooperativity in MTAN Vibrio cholerae
V153I mutatition of Vibrio cholera MTAN catalytic site residues to match those of Escherichia coli MTAN. In the Val153Ile mutant or double mutants, transition-state analogue binding at one site causes complete inhibition. Amino acid, Val153, alters the catalytic site cooperativity in MTAN Vibrio cholerae

Inhibitors

Inhibitors Comment Organism Structure
(3R,4S)-1-[(4-amino-5H-pyrrolo[3,2-d]pyrimidin-7-yl)methyl]-4-[(butylsulfanyl)methyl]pyrrolidin-3-ol
-
Vibrio cholerae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.000061
-
S-adenosyl-L-homocysteine wild-type, pH 7.7, temperature not specified in the publication Vibrio cholerae
0.000202
-
S-adenosyl-L-homocysteine mutant A113P/V153I, pH 7.7, temperature not specified in the publication Vibrio cholerae
0.000262
-
S-adenosyl-L-homocysteine mutant A113P, pH 7.7, temperature not specified in the publication Vibrio cholerae
0.00043
-
S-adenosyl-L-homocysteine mutant V153I, pH 7.7, temperature not specified in the publication Vibrio cholerae

Organism

Organism UniProt Comment Textmining
Vibrio cholerae A5F5R2
-
-
Vibrio cholerae ATCC 39541 A5F5R2
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-homocysteine + H2O
-
Vibrio cholerae S-(5-deoxy-D-ribos-5-yl)-L-homocysteine + adenine
-
?
S-adenosyl-L-homocysteine + H2O
-
Vibrio cholerae ATCC 39541 S-(5-deoxy-D-ribos-5-yl)-L-homocysteine + adenine
-
?

Synonyms

Synonyms Comment Organism
MTAN
-
Vibrio cholerae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0079
-
S-adenosyl-L-homocysteine mutant V153I, pH 7.7, temperature not specified in the publication Vibrio cholerae
0.17
-
S-adenosyl-L-homocysteine mutant A113P, pH 7.7, temperature not specified in the publication Vibrio cholerae
0.18
-
S-adenosyl-L-homocysteine wild-type, pH 7.7, temperature not specified in the publication Vibrio cholerae
0.4
-
S-adenosyl-L-homocysteine mutant A113P/V153I, pH 7.7, temperature not specified in the publication Vibrio cholerae

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
18
-
S-adenosyl-L-homocysteine mutant V153I, pH 7.7, temperature not specified in the publication Vibrio cholerae
400
-
S-adenosyl-L-homocysteine mutant A113P/V153I, pH 7.7, temperature not specified in the publication Vibrio cholerae
700
-
S-adenosyl-L-homocysteine mutant A113P, pH 7.7, temperature not specified in the publication Vibrio cholerae
3000
-
S-adenosyl-L-homocysteine wild-type, pH 7.7, temperature not specified in the publication Vibrio cholerae