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Literature summary for 3.2.2.9 extracted from

  • Mishra, V.; Ronning, D.R.
    Crystal structures of the Helicobacter pylori MTAN enzyme reveal specific interactions between S-adenosylhomocysteine and the 5-alkylthio binding subsite (2012), Biochemistry, 51, 9763-9772.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) Rosetta cells Helicobacter pylori

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method. Inactive D198N mutant bound to S-adenosyl-L-homocysteine and active enzyme bound to S-(5-deoxy-D-ribos-5-yl)-L-homocysteine and adenine using 0.05 M magnesium chloride hexahydrate, 0.1 M HEPES (pH 7.5), and 30% (v/v) PEG-MME 550 Helicobacter pylori

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01
-
S-adenosyl-L-homocysteine wild type enzyme, in 100 mM HEPES and 50 mM KCl buffer (pH 7.2), at 37°C Helicobacter pylori
0.014
-
S-adenosyl-L-homocysteine mutant enzyme H109A, in 100 mM HEPES and 50 mM KCl buffer (pH 7.2), at 37°C Helicobacter pylori
0.019
-
S-adenosyl-L-homocysteine mutant enzyme F107A, in 100 mM HEPES and 50 mM KCl buffer (pH 7.2), at 37°C Helicobacter pylori

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-homocysteine + H2O Helicobacter pylori
-
S-(5-deoxy-D-ribos-5-yl)-L-homocysteine + adenine
-
?

Organism

Organism UniProt Comment Textmining
Helicobacter pylori
-
-
-

Purification (Commentary)

Purification (Comment) Organism
HisTrap column chromatography and Superdex 200 gel filtration Helicobacter pylori

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-homocysteine + H2O
-
Helicobacter pylori S-(5-deoxy-D-ribos-5-yl)-L-homocysteine + adenine
-
?

Synonyms

Synonyms Comment Organism
5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase
-
Helicobacter pylori
MTAN
-
Helicobacter pylori

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.44
-
S-adenosyl-L-homocysteine mutant enzyme F107A, in 100 mM HEPES and 50 mM KCl buffer (pH 7.2), at 37°C Helicobacter pylori
0.74
-
S-adenosyl-L-homocysteine mutant enzyme H109A, in 100 mM HEPES and 50 mM KCl buffer (pH 7.2), at 37°C Helicobacter pylori
1.8
-
S-adenosyl-L-homocysteine wild type enzyme, in 100 mM HEPES and 50 mM KCl buffer (pH 7.2), at 37°C Helicobacter pylori

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
23
-
S-adenosyl-L-homocysteine mutant enzyme F107A, in 100 mM HEPES and 50 mM KCl buffer (pH 7.2), at 37°C Helicobacter pylori
51
-
S-adenosyl-L-homocysteine mutant enzyme H109A, in 100 mM HEPES and 50 mM KCl buffer (pH 7.2), at 37°C Helicobacter pylori
170
-
S-adenosyl-L-homocysteine wild type enzyme, in 100 mM HEPES and 50 mM KCl buffer (pH 7.2), at 37°C Helicobacter pylori