Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) Rosetta cells | Helicobacter pylori |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method. Inactive D198N mutant bound to S-adenosyl-L-homocysteine and active enzyme bound to S-(5-deoxy-D-ribos-5-yl)-L-homocysteine and adenine using 0.05 M magnesium chloride hexahydrate, 0.1 M HEPES (pH 7.5), and 30% (v/v) PEG-MME 550 | Helicobacter pylori |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
S-adenosyl-L-homocysteine | wild type enzyme, in 100 mM HEPES and 50 mM KCl buffer (pH 7.2), at 37°C | Helicobacter pylori | |
0.014 | - |
S-adenosyl-L-homocysteine | mutant enzyme H109A, in 100 mM HEPES and 50 mM KCl buffer (pH 7.2), at 37°C | Helicobacter pylori | |
0.019 | - |
S-adenosyl-L-homocysteine | mutant enzyme F107A, in 100 mM HEPES and 50 mM KCl buffer (pH 7.2), at 37°C | Helicobacter pylori |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-homocysteine + H2O | Helicobacter pylori | - |
S-(5-deoxy-D-ribos-5-yl)-L-homocysteine + adenine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Helicobacter pylori | - |
- |
- |
Purification (Comment) | Organism |
---|---|
HisTrap column chromatography and Superdex 200 gel filtration | Helicobacter pylori |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-homocysteine + H2O | - |
Helicobacter pylori | S-(5-deoxy-D-ribos-5-yl)-L-homocysteine + adenine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase | - |
Helicobacter pylori |
MTAN | - |
Helicobacter pylori |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.44 | - |
S-adenosyl-L-homocysteine | mutant enzyme F107A, in 100 mM HEPES and 50 mM KCl buffer (pH 7.2), at 37°C | Helicobacter pylori | |
0.74 | - |
S-adenosyl-L-homocysteine | mutant enzyme H109A, in 100 mM HEPES and 50 mM KCl buffer (pH 7.2), at 37°C | Helicobacter pylori | |
1.8 | - |
S-adenosyl-L-homocysteine | wild type enzyme, in 100 mM HEPES and 50 mM KCl buffer (pH 7.2), at 37°C | Helicobacter pylori |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
23 | - |
S-adenosyl-L-homocysteine | mutant enzyme F107A, in 100 mM HEPES and 50 mM KCl buffer (pH 7.2), at 37°C | Helicobacter pylori | |
51 | - |
S-adenosyl-L-homocysteine | mutant enzyme H109A, in 100 mM HEPES and 50 mM KCl buffer (pH 7.2), at 37°C | Helicobacter pylori | |
170 | - |
S-adenosyl-L-homocysteine | wild type enzyme, in 100 mM HEPES and 50 mM KCl buffer (pH 7.2), at 37°C | Helicobacter pylori |