Crystallization (Comment) | Organism |
---|---|
enzyme alone, in complex with formycin A and in complex with adenine, hanging drop vapor diffusion method, using 0.1 M Tris pH 8.5 and 16% (w/v) polyethyleneglycol 8000, at 18°C | Helicobacter pylori |
enzyme only or in complex with formycin A or adenine, hanging drop vapor diffusion method, using 0.1 M Tris pH 8.5 and 16% (w/v) polyethyleneglycol 8000, at 18°C | Helicobacter pylori |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Tris | weak inhibitor; weak inhibitor, about 20% residual activity at 250 mM | Helicobacter pylori |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0449 | - |
5'-methylthioadenosine | at 37°C in 100 mM HEPES, 50 mM KCl, pH 7.5 | Helicobacter pylori |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-homocysteine + H2O | Helicobacter pylori | - |
S-(5-deoxy-D-ribos-5-yl)-L-homocysteine + adenine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Helicobacter pylori | - |
- |
- |
Helicobacter pylori | Q9ZMY2 | - |
- |
Purification (Comment) | Organism |
---|---|
HisTrap column chromatography and Superdex 200 gel filtration | Helicobacter pylori |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5'-deoxyadenosine + H2O | - |
Helicobacter pylori | 5-deoxy-D-ribose + adenine | - |
? | |
5'-methylthioadenosine + H2O | - |
Helicobacter pylori | 5-methylthio-D-ribose + adenine | - |
? | |
S-adenosyl-L-homocysteine + H2O | - |
Helicobacter pylori | S-(5-deoxy-D-ribos-5-yl)-L-homocysteine + adenine | - |
? | |
S-adenosyl-L-homocysteine + H2O | - |
Helicobacter pylori | S-(5-deoxy-D-ribos-5-yl)-L-homocysteine + adenine | - |
ir |
Subunits | Comment | Organism |
---|---|---|
homodimer | x-ray crystallography | Helicobacter pylori |
Synonyms | Comment | Organism |
---|---|---|
5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase | - |
Helicobacter pylori |
5-methylthioadenosine/S-adenosylhomocysteine nucleosidase | - |
Helicobacter pylori |
MTAN | - |
Helicobacter pylori |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.92 | - |
5'-methylthioadenosine | at 37°C in 100 mM HEPES, 50 mM KCl, pH 7.5 | Helicobacter pylori |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
25.24 | - |
Tris | at 37°C, pH 7.5 | Helicobacter pylori |