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Literature summary for 3.2.2.9 extracted from

  • Li, X.; Apel, D.; Gaynor, E.C.; Tanner, M.E.
    5'-methylthioadenosine nucleosidase is implicated in playing a key role in a modified futalosine pathway for menaquinone biosynthesis in Campylobacter jejuni (2011), J. Biol. Chem., 286, 19392-19398.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Campylobacter jejuni

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00093
-
5'-methylthioadenosine in 50 mM potassium phosphate buffer, pH 7.0, at 25°C Campylobacter jejuni
0.00103
-
6-amino-6-deoxyfutalosine in 50 mM potassium phosphate buffer, pH 7.0, at 25°C Campylobacter jejuni

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5'-methylthioadenosine + H2O Campylobacter jejuni
-
5-methylthio-D-ribose + adenine
-
?
5'-methylthioadenosine + H2O Campylobacter jejuni NCTC 11168
-
5-methylthio-D-ribose + adenine
-
?
S-adenosyl-L-homocysteine + H2O Campylobacter jejuni
-
S-(5-deoxy-D-ribos-5-yl)-L-homocysteine + adenine
-
?
S-adenosyl-L-homocysteine + H2O Campylobacter jejuni NCTC 11168
-
S-(5-deoxy-D-ribos-5-yl)-L-homocysteine + adenine
-
?

Organism

Organism UniProt Comment Textmining
Campylobacter jejuni Q0PC20
-
-
Campylobacter jejuni NCTC 11168 Q0PC20
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-Sepharose column chromatography Campylobacter jejuni

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5'-methylthioadenosine + H2O
-
Campylobacter jejuni 5-methylthio-D-ribose + adenine
-
?
5'-methylthioadenosine + H2O
-
Campylobacter jejuni NCTC 11168 5-methylthio-D-ribose + adenine
-
?
6-amino-6-deoxyfutalosine + H2O
-
Campylobacter jejuni dehypoxanthine futalosine + adenine
-
?
6-amino-6-deoxyfutalosine + H2O
-
Campylobacter jejuni NCTC 11168 dehypoxanthine futalosine + adenine
-
?
S-adenosyl-L-homocysteine + H2O
-
Campylobacter jejuni S-(5-deoxy-D-ribos-5-yl)-L-homocysteine + adenine
-
?
S-adenosyl-L-homocysteine + H2O
-
Campylobacter jejuni NCTC 11168 S-(5-deoxy-D-ribos-5-yl)-L-homocysteine + adenine
-
?

Synonyms

Synonyms Comment Organism
5'-methylthioadenosine nucleosidase
-
Campylobacter jejuni
Cj0117
-
Campylobacter jejuni
MTAN
-
Campylobacter jejuni

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.53
-
6-amino-6-deoxyfutalosine in 50 mM potassium phosphate buffer, pH 7.0, at 25°C Campylobacter jejuni
2.7
-
5'-methylthioadenosine in 50 mM potassium phosphate buffer, pH 7.0, at 25°C Campylobacter jejuni

General Information

General Information Comment Organism
physiological function the enzyme plays key roles in S-adenosylmethionine recycling, the biosynthesis of autoinducer molecules, and the biosynthesis of menaquinone Campylobacter jejuni