Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Campylobacter jejuni |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00093 | - |
5'-methylthioadenosine | in 50 mM potassium phosphate buffer, pH 7.0, at 25°C | Campylobacter jejuni | |
0.00103 | - |
6-amino-6-deoxyfutalosine | in 50 mM potassium phosphate buffer, pH 7.0, at 25°C | Campylobacter jejuni |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
5'-methylthioadenosine + H2O | Campylobacter jejuni | - |
5-methylthio-D-ribose + adenine | - |
? | |
5'-methylthioadenosine + H2O | Campylobacter jejuni NCTC 11168 | - |
5-methylthio-D-ribose + adenine | - |
? | |
S-adenosyl-L-homocysteine + H2O | Campylobacter jejuni | - |
S-(5-deoxy-D-ribos-5-yl)-L-homocysteine + adenine | - |
? | |
S-adenosyl-L-homocysteine + H2O | Campylobacter jejuni NCTC 11168 | - |
S-(5-deoxy-D-ribos-5-yl)-L-homocysteine + adenine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Campylobacter jejuni | Q0PC20 | - |
- |
Campylobacter jejuni NCTC 11168 | Q0PC20 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-Sepharose column chromatography | Campylobacter jejuni |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5'-methylthioadenosine + H2O | - |
Campylobacter jejuni | 5-methylthio-D-ribose + adenine | - |
? | |
5'-methylthioadenosine + H2O | - |
Campylobacter jejuni NCTC 11168 | 5-methylthio-D-ribose + adenine | - |
? | |
6-amino-6-deoxyfutalosine + H2O | - |
Campylobacter jejuni | dehypoxanthine futalosine + adenine | - |
? | |
6-amino-6-deoxyfutalosine + H2O | - |
Campylobacter jejuni NCTC 11168 | dehypoxanthine futalosine + adenine | - |
? | |
S-adenosyl-L-homocysteine + H2O | - |
Campylobacter jejuni | S-(5-deoxy-D-ribos-5-yl)-L-homocysteine + adenine | - |
? | |
S-adenosyl-L-homocysteine + H2O | - |
Campylobacter jejuni NCTC 11168 | S-(5-deoxy-D-ribos-5-yl)-L-homocysteine + adenine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
5'-methylthioadenosine nucleosidase | - |
Campylobacter jejuni |
Cj0117 | - |
Campylobacter jejuni |
MTAN | - |
Campylobacter jejuni |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.53 | - |
6-amino-6-deoxyfutalosine | in 50 mM potassium phosphate buffer, pH 7.0, at 25°C | Campylobacter jejuni | |
2.7 | - |
5'-methylthioadenosine | in 50 mM potassium phosphate buffer, pH 7.0, at 25°C | Campylobacter jejuni |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme plays key roles in S-adenosylmethionine recycling, the biosynthesis of autoinducer molecules, and the biosynthesis of menaquinone | Campylobacter jejuni |