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Literature summary for 3.2.2.9 extracted from
- S-Adenosylhomocysteine nucleosidase (Escherichia coli) (1971), Methods Enzymol., 17, 411-415.
No PubMed abstract available
General Stability
| General Stability | Organism |
|---|---|
| unstable in solution of low ionic strength | Escherichia coli |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 5.4 | - |
5'-methylthioadenosine | - |
Escherichia coli |  |
| 9 | - |
S-adenosylhomocysteine | - |
Escherichia coli | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5'-methylthioadenosine + H2O | Salmonella enterica subsp. enterica serovar Typhimurium | - |
? | - |
? | |
| 5'-methylthioadenosine + H2O | Escherichia coli | - |
? | - |
? | |
| 5'-methylthioadenosine + H2O | Klebsiella aerogenes | - |
? | - |
? | |
| S-adenosyl-L-homocysteine + H2O | Salmonella enterica subsp. enterica serovar Typhimurium | - |
? | - |
? | |
| S-adenosyl-L-homocysteine + H2O | Escherichia coli | - |
? | - |
? | |
| S-adenosyl-L-homocysteine + H2O | Klebsiella aerogenes | - |
? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
| Klebsiella aerogenes | - |
- |
- |
| Salmonella enterica subsp. enterica serovar Typhimurium | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 3.6 | - |
- |
Escherichia coli |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 0°C, 0.1 M phosphate buffer containing 0.009 M 2-mercaptoethanol and 0.0005 M EDTA stable for 2 weeks, retains 80% of its total activity after 2 weeks at -40°C | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5'-methylthioadenosine + H2O | - |
Salmonella enterica subsp. enterica serovar Typhimurium | 5-methylthio-D-ribose + adenine | - |
? | |
| 5'-methylthioadenosine + H2O | - |
Escherichia coli | 5-methylthio-D-ribose + adenine | - |
? | |
| 5'-methylthioadenosine + H2O | - |
Klebsiella aerogenes | 5-methylthio-D-ribose + adenine | - |
? | |
| 5'-methylthioadenosine + H2O | - |
Salmonella enterica subsp. enterica serovar Typhimurium | ? | - |
? | |
| 5'-methylthioadenosine + H2O | - |
Escherichia coli | ? | - |
? | |
| 5'-methylthioadenosine + H2O | - |
Klebsiella aerogenes | ? | - |
? | |
| S-adenosyl-L-homocysteine + H2O | - |
Salmonella enterica subsp. enterica serovar Typhimurium | adenine + S-ribosyl-L-homocysteine | - |
? | |
| S-adenosyl-L-homocysteine + H2O | - |
Escherichia coli | adenine + S-ribosyl-L-homocysteine | - |
? | |
| S-adenosyl-L-homocysteine + H2O | - |
Klebsiella aerogenes | adenine + S-ribosyl-L-homocysteine | - |
? | |
| S-adenosyl-L-homocysteine + H2O | - |
Salmonella enterica subsp. enterica serovar Typhimurium | ? | - |
? | |
| S-adenosyl-L-homocysteine + H2O | - |
Escherichia coli | ? | - |
? | |
| S-adenosyl-L-homocysteine + H2O | - |
Klebsiella aerogenes | ? | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
optimal pH for both natural substrates, 5'-methylthioadenosine and S-adenosyl-L-homocysteine, with little variation from pH 5.7-7.1 | Escherichia coli |
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Release 2023.1 (January 2023)
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