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Literature summary for 3.2.2.9 extracted from

  • Duerre, J.A.
    A hydrolytic nucleosidase acting on S-adenosylhomocysteine and on 5'-methylthioadenosine (1962), J. Biol. Chem., 237, 3737-3741.
No PubMed abstract available
Search for more literature for 3.2.2.9

General Stability

General Stability Organism
unstable in solution of low ionic strength Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.4
-
 5'-methylthioadenosine
-
 Escherichia coli
9
-
 S-adenosylhomocysteine
-
 Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5'-methylthioadenosine + H2O Salmonella enterica subsp. enterica serovar Typhimurium
-
 ?
-
 ?
5'-methylthioadenosine + H2O Escherichia coli
-
 ?
-
 ?
5'-methylthioadenosine + H2O Klebsiella aerogenes
-
 ?
-
 ?
5'-methylthioadenosine + H2O Escherichia coli B / ATCC 11303
-
 ?
-
 ?
S-adenosyl-L-homocysteine + H2O Salmonella enterica subsp. enterica serovar Typhimurium
-
 ?
-
 ?
S-adenosyl-L-homocysteine + H2O Escherichia coli
-
 ?
-
 ?
S-adenosyl-L-homocysteine + H2O Klebsiella aerogenes
-
 ?
-
 ?
S-adenosyl-L-homocysteine + H2O Escherichia coli B / ATCC 11303
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Escherichia coli B / ATCC 11303
-
-
-
Klebsiella aerogenes
-
 NRRL No. 199
-
Salmonella enterica subsp. enterica serovar Typhimurium
-
-
-

Purification (Commentary)

Purification (Comment) Organism
160-fold Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.75
-
 substrate S-adenosyl-L-homocysteine Escherichia coli

Storage Stability

Storage Stability Organism
0°C, loses 50% activity in 48 h, -20°C very unstable, lost nearly all activity when stored for 1 week Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5'-methylthioadenosine + H2O
-
 Salmonella enterica subsp. enterica serovar Typhimurium 5-methylthio-D-ribose + adenine
-
 ?
5'-methylthioadenosine + H2O
-
 Escherichia coli 5-methylthio-D-ribose + adenine
-
 ?
5'-methylthioadenosine + H2O
-
 Klebsiella aerogenes 5-methylthio-D-ribose + adenine
-
 ?
5'-methylthioadenosine + H2O
-
 Escherichia coli B / ATCC 11303 5-methylthio-D-ribose + adenine
-
 ?
5'-methylthioadenosine + H2O
-
 Salmonella enterica subsp. enterica serovar Typhimurium ?
-
 ?
5'-methylthioadenosine + H2O
-
 Escherichia coli ?
-
 ?
5'-methylthioadenosine + H2O
-
 Klebsiella aerogenes ?
-
 ?
5'-methylthioadenosine + H2O
-
 Escherichia coli B / ATCC 11303 ?
-
 ?
S-adenosyl-L-homocysteine + H2O
-
 Salmonella enterica subsp. enterica serovar Typhimurium adenine + S-ribosyl-L-homocysteine
-
 ?
S-adenosyl-L-homocysteine + H2O
-
 Escherichia coli adenine + S-ribosyl-L-homocysteine
-
 ?
S-adenosyl-L-homocysteine + H2O
-
 Klebsiella aerogenes adenine + S-ribosyl-L-homocysteine
-
 ?
S-adenosyl-L-homocysteine + H2O
-
 Escherichia coli B / ATCC 11303 adenine + S-ribosyl-L-homocysteine
-
 ?
S-adenosyl-L-homocysteine + H2O
-
 Salmonella enterica subsp. enterica serovar Typhimurium ?
-
 ?
S-adenosyl-L-homocysteine + H2O
-
 Escherichia coli ?
-
 ?
S-adenosyl-L-homocysteine + H2O
-
 Klebsiella aerogenes ?
-
 ?
S-adenosyl-L-homocysteine + H2O
-
 Escherichia coli B / ATCC 11303 ?
-
 ?
S-adenosylmethionine + H2O
-
 Klebsiella aerogenes adenine + hypoxanthine + methylthioribose + homoserine lactone
-
 ?

Synonyms

Synonyms Comment Organism
nucleosidase
-
 Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
 optimal pH for both natural substrates, 5'-methylthioadenosine and S-adenosyl-L-homocysteine, with little variation from pH 5.7-7.1 Escherichia coli

pH Range

pH Minimum pH Maximum Comment Organism
4 8.5
-
 Escherichia coli