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Literature summary for 3.2.2.6 extracted from

  • Jiang, H.; Sherwood, R.; Zhang, S.; Zhu, X.; Liu, Q.; Graeff, R.; Kriksunov, I.A.; Lee, H.C.; Hao, Q.; Lin, H.
    Identification of ADP-ribosylation sites of CD38 mutants by precursor ion scanning mass spectrometry (2013), Anal. Biochem., 433, 218-226.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.2.6

Protein Variants

Protein Variants Comment Organism
E226D site-directed mutagenesis, catalytic mutant Homo sapiens
E226Q site-directed mutagenesis, catalytic mutant Homo sapiens
N100D site-directed mutagenesis, N-glycoylation at the site is abolished Homo sapiens
N164D site-directed mutagenesis, N-glycoylation at the site is abolished Homo sapiens
N209D site-directed mutagenesis, N-glycoylation at the site is abolished Homo sapiens
N219D site-directed mutagenesis, N-glycoylation at the site is abolished Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
NAD+ + H2O Homo sapiens via a stepwise reaction mechanism ADP-D-ribose + nicotinamide
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens P28907
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein N-glycosylation sites are N100, N164, N209, and N219 Homo sapiens

Reaction

Reaction Comment Organism Reaction ID
NAD+ + H2O = ADP-D-ribose + nicotinamide + H+ reaction mechanism, overview Homo sapiens
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
NAD+ + H2O via a stepwise reaction mechanism Homo sapiens ADP-D-ribose + nicotinamide
-
 ?

Synonyms

Synonyms Comment Organism
CD38
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Homo sapiens

General Information

General Information Comment Organism
additional information D226/Q226 and K129 residues of the two CD38 enzyme are the ADP-ribosylation sites. 6-Alkyne-F-araNAD, 6-alkyne-NAD, and Rh-N3 are used in the labeling reactions of CD38 wild-type and mutants, overview Homo sapiens