Literature summary for 3.2.2.5 extracted from

Yoon, J.Y.; An, D.R.; Yoon, H.J.; Kim, H.S.; Lee, S.J.; Im, H.N.; Jang, J.Y.; Suh, S.W.
High-resolution crystal structure of Streptococcus pyogenes beta-NAD+ glycohydrolase in complex with its endogenous inhibitor IFS reveals a highly water-rich interface (2013), J. Synchrotron Radiat., 20, 962-967.

Cloned(Commentary)

Cloned (Comment)	Organism
co-expression of N-terminally His6-tagged enzyme with inhibitor IFS in Escherichia coli strain Rosetta2 (DE3)	Streptococcus pyogenes serotype M3

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified recombinant selenomethionine-labeled enzyme C-terminal domain (residues 193-451) in complex with the full-length inhibitor IFS, sitting drop vapor-diffusion method, mixing of 0.001 ml of protein solution with 0.001 ml of reservoir solution of 20% w/v tacsimate, pH 4.0, and 20% w/v PEG 3350, 22°C, X-ray diffraction structure determination and analysis at 1.70 A resolution, single-wavelength anomalous diffraction, modelling	Streptococcus pyogenes serotype M3

Crystallization (Comment)

Organism

purified recombinant selenomethionine-labeled enzyme C-terminal domain (residues 193-451) in complex with the full-length inhibitor IFS, sitting drop vapor-diffusion method, mixing of 0.001 ml of protein solution with 0.001 ml of reservoir solution of 20% w/v tacsimate, pH 4.0, and 20% w/v PEG 3350, 22°C, X-ray diffraction structure determination and analysis at 1.70 A resolution, single-wavelength anomalous diffraction, modelling

Streptococcus pyogenes serotype M3

Inhibitors

Inhibitors	Comment	Organism	Structure
Streptococcal NAD glycohydrolase inhibitor	the ifs gene, or SpyM3_0129, encodes an endogenous competitive inhibitor for the enzyme. IFS is localized intracellularly and forms a complex with the enzyme. This intracellular complex must be dissociated during export through the cell envelope. The interface between SPNct and IFS is highly rich in water molecules, binding structure, overview	Streptococcus pyogenes serotype M3

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
additional information	the enzyme is injected across the host cell membrane into the cytoplasm through streptolysin O, a member of a large family of pore-forming toxins and cholesterol-dependent cytolysins	Streptococcus pyogenes serotype M3	-	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
49000	-	x * 49000, recombinant His6-tagged enzyme, SDS-PAGE	Streptococcus pyogenes serotype M3

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
NAD+ + H2O	Streptococcus pyogenes serotype M3	-	ADP-D-ribose + nicotinamide	-	?

Organism

Organism	UniProt	Comment	Textmining
Streptococcus pyogenes serotype M3	A0A0H2UTN5	gene spn or SpyM3_0128	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant N-terminally His6-tagged enzyme from Escherichia coli strain Rosetta2 (DE3) by nickel affinity chromatography and gel filtration	Streptococcus pyogenes serotype M3

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	lack of ADP-ribosyltransferase activity of the enzyme	Streptococcus pyogenes serotype M3	?	-	?
NAD+ + H2O	-	Streptococcus pyogenes serotype M3	ADP-D-ribose + nicotinamide	-	?

Subunits

Subunits	Comment	Organism
?	x * 49000, recombinant His6-tagged enzyme, SDS-PAGE	Streptococcus pyogenes serotype M3

Synonyms

Synonyms	Comment	Organism
beta-NAD+glycohydrolase	-	Streptococcus pyogenes serotype M3
SPN	-	Streptococcus pyogenes serotype M3

General Information

General Information	Comment	Organism
physiological function	the enzyme is one of the virulence factors produced by Streptococcus pyogenes, which injects the enzyme into the cytosol of an infected host cell using the cytolysin-mediated translocation pathway. For the export of the enzyme through the cell envelope, the intracellular complex with inhibitor IFN must be dissociated	Streptococcus pyogenes serotype M3