Literature summary for 3.2.2.5 extracted from

Smith, C.L.; Ghosh, J.; Elam, J.S.; Pinkner, J.S.; Hultgren, S.J.; Caparon, M.G.; Ellenberger, T.
Structural basis of Streptococcus pyogenes immunity to its NAD+ glycohydrolase toxin (2011), Structure, 19, 192-202.

Search for more literature for 3.2.2.5

Cloned(Commentary)

Cloned (Comment)	Organism
the C-terminal glycohydrolase domain of SPN (residues 191-451) in complex with IFS is expressed in Escherichia coli strain DL41	Streptococcus pyogenes

Crystallization (Commentary)

Crystallization (Comment)	Organism
C-terminal glycohydrolase domain of SPN (residues 191-451) in complex with IFS, vapor diffusion method, using 20%-25% (w/v) PEG 8K and 100 mM Tris-HCl (pH 8.0), at 22°C	Streptococcus pyogenes

Organism

Organism	UniProt	Comment	Textmining
Streptococcus pyogenes	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
Talon metal affinity column chromatography, Q-Sepharose column chromatography, and S-200 gel filtration	Streptococcus pyogenes

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
beta-NAD+ + H2O	-	Streptococcus pyogenes	ADP-ribose + nicotinamide + H+	-	?

Synonyms

Synonyms	Comment	Organism
beta-NAD+ glycohydrolase	-	Streptococcus pyogenes
SPN	-	Streptococcus pyogenes

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)